Aprobation of platelet aggregation inhibitor from Echis multisquamatis snake venom in vitro, in vivo and ex vivo
Snake venom-derived platelet aggregation inhibitors can be promising antiplatelet medications that can allow to avoid the risk of bleeding and treatment resistance, particularly in aspirin-resistant patients. Our study aimed to assess the effectiveness of a platelet aggregation inhibitor derived fro...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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National Academy of Sciences of Ukraine, Palladin Institute of Biochemistry
2023-10-01
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| Series: | Biotechnologia Acta |
| Subjects: | |
| Online Access: | https://biotechnology.kiev.ua/images/BTA/2023/5_2023/Zhelavskyi5_2023.pdf |
| _version_ | 1797392388510973952 |
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| author | Zhelavskyi M. A. Platonov M. O. Kucheryavyi Y. Р. Stohnii Y. M. |
| author_facet | Zhelavskyi M. A. Platonov M. O. Kucheryavyi Y. Р. Stohnii Y. M. |
| author_sort | Zhelavskyi M. A. |
| collection | DOAJ |
| description | Snake venom-derived platelet aggregation inhibitors can be promising antiplatelet medications that can allow to avoid the risk of bleeding and treatment resistance, particularly in aspirin-resistant patients. Our study aimed to assess the effectiveness of a platelet aggregation inhibitor derived from Echis multisquamatis snake venom in various settings, including in vitro, in vivo, and ex vivo.
Methods. We examined a polypeptide from Echis multisquamatis venom, purified using a recently developed chromatography protocol, across multiple models. This polypeptide was introduced into platelet-rich blood plasma and administered intravenously to rats. The effects on platelet aggregation were assessed using aggregometry, focusing on ADP-induced aggregation.
Results & Discussion. Our findings revealed that a concentration of 0.040 mg/ml significantly reduced platelet aggregation in vitro. Remarkably, this dosage also proved effective when administered intravenously in laboratory animals, reaffirming its potential as a robust antiplatelet agent. In the final phase of our study, the polypeptide demonstrated its ability to inhibit platelet aggregation in blood plasma of pregnant woman with aspirin resistance, presenting a promising avenue for innovative treatment approaches in such cases.
Conclusion. This study underscores the potential of the Echis multisquamatis venom-derived polypeptide as a promising antiplatelet agent, effective in diverse scenarios, including aspirin resistance. Further research and clinical trials are imperative to fully harness its therapeutic potential. |
| first_indexed | 2024-03-08T23:46:31Z |
| format | Article |
| id | doaj.art-4e86bd642e844ba5aff95a658d23a0df |
| institution | Directory Open Access Journal |
| issn | 2410-7751 2410-776X |
| language | English |
| last_indexed | 2024-03-08T23:46:31Z |
| publishDate | 2023-10-01 |
| publisher | National Academy of Sciences of Ukraine, Palladin Institute of Biochemistry |
| record_format | Article |
| series | Biotechnologia Acta |
| spelling | doaj.art-4e86bd642e844ba5aff95a658d23a0df2023-12-13T16:58:33ZengNational Academy of Sciences of Ukraine, Palladin Institute of BiochemistryBiotechnologia Acta2410-77512410-776X2023-10-01165556010.15407/biotech16.05.055Aprobation of platelet aggregation inhibitor from Echis multisquamatis snake venom in vitro, in vivo and ex vivoZhelavskyi M. A.0Platonov M. O.1Kucheryavyi Y. Р.2Stohnii Y. M.3Palladin Institute of biochemistry of National Academy of Sciences of Ukraine, Kyiv; ZL “Success Academy”, Kyiv region, UkrainePalladin Institute of biochemistry of National Academy of Sciences of Ukraine, Kyiv Palladin Institute of biochemistry of National Academy of Sciences of Ukraine, Kyiv Palladin Institute of biochemistry of National Academy of Sciences of Ukraine, Kyiv Snake venom-derived platelet aggregation inhibitors can be promising antiplatelet medications that can allow to avoid the risk of bleeding and treatment resistance, particularly in aspirin-resistant patients. Our study aimed to assess the effectiveness of a platelet aggregation inhibitor derived from Echis multisquamatis snake venom in various settings, including in vitro, in vivo, and ex vivo. Methods. We examined a polypeptide from Echis multisquamatis venom, purified using a recently developed chromatography protocol, across multiple models. This polypeptide was introduced into platelet-rich blood plasma and administered intravenously to rats. The effects on platelet aggregation were assessed using aggregometry, focusing on ADP-induced aggregation. Results & Discussion. Our findings revealed that a concentration of 0.040 mg/ml significantly reduced platelet aggregation in vitro. Remarkably, this dosage also proved effective when administered intravenously in laboratory animals, reaffirming its potential as a robust antiplatelet agent. In the final phase of our study, the polypeptide demonstrated its ability to inhibit platelet aggregation in blood plasma of pregnant woman with aspirin resistance, presenting a promising avenue for innovative treatment approaches in such cases. Conclusion. This study underscores the potential of the Echis multisquamatis venom-derived polypeptide as a promising antiplatelet agent, effective in diverse scenarios, including aspirin resistance. Further research and clinical trials are imperative to fully harness its therapeutic potential.https://biotechnology.kiev.ua/images/BTA/2023/5_2023/Zhelavskyi5_2023.pdfdisintegrinblood plasmaplateletsthrombosisblood coagulationplatelet aggregationanimal model. |
| spellingShingle | Zhelavskyi M. A. Platonov M. O. Kucheryavyi Y. Р. Stohnii Y. M. Aprobation of platelet aggregation inhibitor from Echis multisquamatis snake venom in vitro, in vivo and ex vivo Biotechnologia Acta disintegrin blood plasma platelets thrombosis blood coagulation platelet aggregation animal model. |
| title | Aprobation of platelet aggregation inhibitor from Echis multisquamatis snake venom in vitro, in vivo and ex vivo |
| title_full | Aprobation of platelet aggregation inhibitor from Echis multisquamatis snake venom in vitro, in vivo and ex vivo |
| title_fullStr | Aprobation of platelet aggregation inhibitor from Echis multisquamatis snake venom in vitro, in vivo and ex vivo |
| title_full_unstemmed | Aprobation of platelet aggregation inhibitor from Echis multisquamatis snake venom in vitro, in vivo and ex vivo |
| title_short | Aprobation of platelet aggregation inhibitor from Echis multisquamatis snake venom in vitro, in vivo and ex vivo |
| title_sort | aprobation of platelet aggregation inhibitor from echis multisquamatis snake venom in vitro in vivo and ex vivo |
| topic | disintegrin blood plasma platelets thrombosis blood coagulation platelet aggregation animal model. |
| url | https://biotechnology.kiev.ua/images/BTA/2023/5_2023/Zhelavskyi5_2023.pdf |
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