Immunomodulatory activities of isolated compounds from the root-bark of Cussonia arborea
Context: Cussonia arborea Hochst. ex A. Rich (Araliaceae) is a folk medicine used to treat various diseases. However, there is no report of the root phytochemistry. Objective: This study isolates and identifies the immunomodulatory compounds from root-bark of C. arborea. Materials and methods: The m...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Taylor & Francis Group
2017-01-01
|
| Series: | Pharmaceutical Biology |
| Subjects: | |
| Online Access: | http://dx.doi.org/10.1080/13880209.2017.1400078 |
| _version_ | 1818027863008346112 |
|---|---|
| author | Abdulkabir Oladele Oladimeji Ibrahim Adebayo Oladosu Almas Jabeen Aisha Faheem Mohammed Ahmed Mesaik Muhammad Shaiq Ali |
| author_facet | Abdulkabir Oladele Oladimeji Ibrahim Adebayo Oladosu Almas Jabeen Aisha Faheem Mohammed Ahmed Mesaik Muhammad Shaiq Ali |
| author_sort | Abdulkabir Oladele Oladimeji |
| collection | DOAJ |
| description | Context: Cussonia arborea Hochst. ex A. Rich (Araliaceae) is a folk medicine used to treat various diseases. However, there is no report of the root phytochemistry. Objective: This study isolates and identifies the immunomodulatory compounds from root-bark of C. arborea. Materials and methods: The methanol extract (18 g) was subjected to repeated column chromatography resulting in isolation of five compounds (1–5). Structure determination was achieved by analysis of their 1 D and 2 D NMR, and mass spectroscopy. The compounds (100–1.0 μg/mL) were examined immunomodulatory for effect on production of reactive oxygen species (ROS) from whole blood phagocytes and on proliferation of T-cells. The compounds cytotoxicity (100–1.0 μg/mL) was evaluated on NIH-3T3 normal fibroblast cells. Results: Three pentacyclic triterpenoids [3, 23-dihydroxy-12-oleanen-28-oic acid (1), 3β-hydroxylolean-12-en-28-oic (2) and 23-hydoxy-oxo-urs-12-en-28-oic acid (5)], two phytosterols: [stigmasterol (3)] and [3-O-β-d-glucopyranosyl stigmasterol (4)] were all isolated from the methanol soluble extract. All the tested compounds (1–4) were found to be nontoxic on NIH-3T3 cells. Compound 1 and 2 moderately inhibited the production of ROS (IC50 = 24.4 ± 4.3 and 37.5 ± 0.1 μg/mL, respectively) whereas compound 2 exhibited the highest inhibitory effect (IC50 = 12.6 ± 0.4 μg/mL) on proliferation of phytoheamagglutinin (PHA) activated T-cells. Conclusions: The isolated compounds (1–5) are reported for the first time from this species. In addition, compound 2 with suppressive potential on production of intracellular ROS and proliferation of T-cells could be of immense value in control of autoimmune diseases as well as in immune compromised patients. |
| first_indexed | 2024-12-10T04:54:39Z |
| format | Article |
| id | doaj.art-4e87351c819c4c759e1c52b89673b8aa |
| institution | Directory Open Access Journal |
| issn | 1388-0209 1744-5116 |
| language | English |
| last_indexed | 2024-12-10T04:54:39Z |
| publishDate | 2017-01-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Pharmaceutical Biology |
| spelling | doaj.art-4e87351c819c4c759e1c52b89673b8aa2022-12-22T02:01:32ZengTaylor & Francis GroupPharmaceutical Biology1388-02091744-51162017-01-015512240224710.1080/13880209.2017.14000781400078Immunomodulatory activities of isolated compounds from the root-bark of Cussonia arboreaAbdulkabir Oladele Oladimeji0Ibrahim Adebayo Oladosu1Almas Jabeen2Aisha Faheem3Mohammed Ahmed Mesaik4Muhammad Shaiq Ali5Ondo State University of Science and TechnologyUniversity of IbadanUniversity of KarachiUniversity of KarachiUniversity of KarachiUniversity of KarachiContext: Cussonia arborea Hochst. ex A. Rich (Araliaceae) is a folk medicine used to treat various diseases. However, there is no report of the root phytochemistry. Objective: This study isolates and identifies the immunomodulatory compounds from root-bark of C. arborea. Materials and methods: The methanol extract (18 g) was subjected to repeated column chromatography resulting in isolation of five compounds (1–5). Structure determination was achieved by analysis of their 1 D and 2 D NMR, and mass spectroscopy. The compounds (100–1.0 μg/mL) were examined immunomodulatory for effect on production of reactive oxygen species (ROS) from whole blood phagocytes and on proliferation of T-cells. The compounds cytotoxicity (100–1.0 μg/mL) was evaluated on NIH-3T3 normal fibroblast cells. Results: Three pentacyclic triterpenoids [3, 23-dihydroxy-12-oleanen-28-oic acid (1), 3β-hydroxylolean-12-en-28-oic (2) and 23-hydoxy-oxo-urs-12-en-28-oic acid (5)], two phytosterols: [stigmasterol (3)] and [3-O-β-d-glucopyranosyl stigmasterol (4)] were all isolated from the methanol soluble extract. All the tested compounds (1–4) were found to be nontoxic on NIH-3T3 cells. Compound 1 and 2 moderately inhibited the production of ROS (IC50 = 24.4 ± 4.3 and 37.5 ± 0.1 μg/mL, respectively) whereas compound 2 exhibited the highest inhibitory effect (IC50 = 12.6 ± 0.4 μg/mL) on proliferation of phytoheamagglutinin (PHA) activated T-cells. Conclusions: The isolated compounds (1–5) are reported for the first time from this species. In addition, compound 2 with suppressive potential on production of intracellular ROS and proliferation of T-cells could be of immense value in control of autoimmune diseases as well as in immune compromised patients.http://dx.doi.org/10.1080/13880209.2017.1400078araliaceaet-cell proliferation assayreactive oxygen speciestriterpenoidsoleanolic acidhederageninstigmasterolnmr spectroscopysteroid |
| spellingShingle | Abdulkabir Oladele Oladimeji Ibrahim Adebayo Oladosu Almas Jabeen Aisha Faheem Mohammed Ahmed Mesaik Muhammad Shaiq Ali Immunomodulatory activities of isolated compounds from the root-bark of Cussonia arborea Pharmaceutical Biology araliaceae t-cell proliferation assay reactive oxygen species triterpenoids oleanolic acid hederagenin stigmasterol nmr spectroscopy steroid |
| title | Immunomodulatory activities of isolated compounds from the root-bark of Cussonia arborea |
| title_full | Immunomodulatory activities of isolated compounds from the root-bark of Cussonia arborea |
| title_fullStr | Immunomodulatory activities of isolated compounds from the root-bark of Cussonia arborea |
| title_full_unstemmed | Immunomodulatory activities of isolated compounds from the root-bark of Cussonia arborea |
| title_short | Immunomodulatory activities of isolated compounds from the root-bark of Cussonia arborea |
| title_sort | immunomodulatory activities of isolated compounds from the root bark of cussonia arborea |
| topic | araliaceae t-cell proliferation assay reactive oxygen species triterpenoids oleanolic acid hederagenin stigmasterol nmr spectroscopy steroid |
| url | http://dx.doi.org/10.1080/13880209.2017.1400078 |
| work_keys_str_mv | AT abdulkabiroladeleoladimeji immunomodulatoryactivitiesofisolatedcompoundsfromtherootbarkofcussoniaarborea AT ibrahimadebayooladosu immunomodulatoryactivitiesofisolatedcompoundsfromtherootbarkofcussoniaarborea AT almasjabeen immunomodulatoryactivitiesofisolatedcompoundsfromtherootbarkofcussoniaarborea AT aishafaheem immunomodulatoryactivitiesofisolatedcompoundsfromtherootbarkofcussoniaarborea AT mohammedahmedmesaik immunomodulatoryactivitiesofisolatedcompoundsfromtherootbarkofcussoniaarborea AT muhammadshaiqali immunomodulatoryactivitiesofisolatedcompoundsfromtherootbarkofcussoniaarborea |