Involvement of Nitrogen on Flavonoids, Glutathione, Anthocyanin, Ascorbic Acid and Antioxidant Activities of Malaysian Medicinal Plant Labisia pumila Blume (Kacip Fatimah)
A split plot 3 by 4 experiment was designed to characterize the relationship between production of gluthatione (GSH), oxidized gluthatione (GSSG), total flavonoid, anthocyanin, ascorbic acid and antioxidant activities (FRAP and DPPH) in three varieties of Labisia pumila Blume, namely the varieties a...
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MDPI AG
2011-12-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | http://www.mdpi.com/1422-0067/13/1/393/ |
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| author | Zaharah Abdul Rahman Hawa Z. E. Jaafar Mohd Hafiz Ibrahim Asmah Rahmat |
| author_facet | Zaharah Abdul Rahman Hawa Z. E. Jaafar Mohd Hafiz Ibrahim Asmah Rahmat |
| author_sort | Zaharah Abdul Rahman |
| collection | DOAJ |
| description | A split plot 3 by 4 experiment was designed to characterize the relationship between production of gluthatione (GSH), oxidized gluthatione (GSSG), total flavonoid, anthocyanin, ascorbic acid and antioxidant activities (FRAP and DPPH) in three varieties of Labisia pumila Blume, namely the varieties alata, pumila and lanceolata, under four levels of nitrogen fertilization (0, 90, 180 and 270 kg N/ha) for 15 weeks. The treatment effects were solely contributed by nitrogen application; there was neither varietal nor interaction effects observed. As the nitrogen levels decreased from 270 to 0 kg N/ha, the production of GSH and GSSG, anthocyanin, total flavonoid and ascorbic acid increased steadily. At the highest nitrogen treatment level, L. pumila exhibited significantly lower antioxidant activities (DPPH and FRAP) than those exposed to limited nitrogen growing conditions. Significant positive correlation was obtained between antioxidant activities (DPPH and FRAP), total flavonoid, GSH, GSSG, anthocyanin and ascorbic acid suggesting that an increase in the antioxidative activities in L. pumila under low nitrogen fertilization could be attributed to higher contents of these compounds. From this observation, it could be concluded that in order to avoid negative effects on the quality of L. pumila, it is advisable to avoid excessive application of nitrogen fertilizer when cultivating the herb for its medicinal use. |
| first_indexed | 2024-04-12T03:53:45Z |
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| issn | 1422-0067 |
| language | English |
| last_indexed | 2024-04-12T03:53:45Z |
| publishDate | 2011-12-01 |
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| series | International Journal of Molecular Sciences |
| spelling | doaj.art-4e87799cc4fb431ab6b081a6e7ff10bf2022-12-22T03:48:54ZengMDPI AGInternational Journal of Molecular Sciences1422-00672011-12-0113139340810.3390/ijms13010393Involvement of Nitrogen on Flavonoids, Glutathione, Anthocyanin, Ascorbic Acid and Antioxidant Activities of Malaysian Medicinal Plant Labisia pumila Blume (Kacip Fatimah)Zaharah Abdul RahmanHawa Z. E. JaafarMohd Hafiz IbrahimAsmah RahmatA split plot 3 by 4 experiment was designed to characterize the relationship between production of gluthatione (GSH), oxidized gluthatione (GSSG), total flavonoid, anthocyanin, ascorbic acid and antioxidant activities (FRAP and DPPH) in three varieties of Labisia pumila Blume, namely the varieties alata, pumila and lanceolata, under four levels of nitrogen fertilization (0, 90, 180 and 270 kg N/ha) for 15 weeks. The treatment effects were solely contributed by nitrogen application; there was neither varietal nor interaction effects observed. As the nitrogen levels decreased from 270 to 0 kg N/ha, the production of GSH and GSSG, anthocyanin, total flavonoid and ascorbic acid increased steadily. At the highest nitrogen treatment level, L. pumila exhibited significantly lower antioxidant activities (DPPH and FRAP) than those exposed to limited nitrogen growing conditions. Significant positive correlation was obtained between antioxidant activities (DPPH and FRAP), total flavonoid, GSH, GSSG, anthocyanin and ascorbic acid suggesting that an increase in the antioxidative activities in L. pumila under low nitrogen fertilization could be attributed to higher contents of these compounds. From this observation, it could be concluded that in order to avoid negative effects on the quality of L. pumila, it is advisable to avoid excessive application of nitrogen fertilizer when cultivating the herb for its medicinal use.http://www.mdpi.com/1422-0067/13/1/393/Labisia pumila Blumenitrogen fertilizationplant secondary metabolitesgluthationeDPPH radical scavengingferric reducing antioxidant power |
| spellingShingle | Zaharah Abdul Rahman Hawa Z. E. Jaafar Mohd Hafiz Ibrahim Asmah Rahmat Involvement of Nitrogen on Flavonoids, Glutathione, Anthocyanin, Ascorbic Acid and Antioxidant Activities of Malaysian Medicinal Plant Labisia pumila Blume (Kacip Fatimah) International Journal of Molecular Sciences Labisia pumila Blume nitrogen fertilization plant secondary metabolites gluthatione DPPH radical scavenging ferric reducing antioxidant power |
| title | Involvement of Nitrogen on Flavonoids, Glutathione, Anthocyanin, Ascorbic Acid and Antioxidant Activities of Malaysian Medicinal Plant Labisia pumila Blume (Kacip Fatimah) |
| title_full | Involvement of Nitrogen on Flavonoids, Glutathione, Anthocyanin, Ascorbic Acid and Antioxidant Activities of Malaysian Medicinal Plant Labisia pumila Blume (Kacip Fatimah) |
| title_fullStr | Involvement of Nitrogen on Flavonoids, Glutathione, Anthocyanin, Ascorbic Acid and Antioxidant Activities of Malaysian Medicinal Plant Labisia pumila Blume (Kacip Fatimah) |
| title_full_unstemmed | Involvement of Nitrogen on Flavonoids, Glutathione, Anthocyanin, Ascorbic Acid and Antioxidant Activities of Malaysian Medicinal Plant Labisia pumila Blume (Kacip Fatimah) |
| title_short | Involvement of Nitrogen on Flavonoids, Glutathione, Anthocyanin, Ascorbic Acid and Antioxidant Activities of Malaysian Medicinal Plant Labisia pumila Blume (Kacip Fatimah) |
| title_sort | involvement of nitrogen on flavonoids glutathione anthocyanin ascorbic acid and antioxidant activities of malaysian medicinal plant labisia pumila blume kacip fatimah |
| topic | Labisia pumila Blume nitrogen fertilization plant secondary metabolites gluthatione DPPH radical scavenging ferric reducing antioxidant power |
| url | http://www.mdpi.com/1422-0067/13/1/393/ |
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