Complete genome sequencing and analysis of a Lancefield group G <it>Streptococcus dysgalactiae </it>subsp. <it>equisimilis </it>strain causing streptococcal toxic shock syndrome (STSS)
<p>Abstract</p> <p>Background</p> <p><it>Streptococcus dysgalactiae </it>subsp. <it>equisimilis </it>(SDSE) causes invasive streptococcal infections, including streptococcal toxic shock syndrome (STSS), as does Lancefield group A <it>Strept...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2011-01-01
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| Series: | BMC Genomics |
| Online Access: | http://www.biomedcentral.com/1471-2164/12/17 |
| _version_ | 1818347552201768960 |
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| author | Ubukata Kimiko Yagi Junji Murayama Somay Okumura Kayo Shimomura Yumi Kirikae Teruo Miyoshi-Akiyama Tohru |
| author_facet | Ubukata Kimiko Yagi Junji Murayama Somay Okumura Kayo Shimomura Yumi Kirikae Teruo Miyoshi-Akiyama Tohru |
| author_sort | Ubukata Kimiko |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p><it>Streptococcus dysgalactiae </it>subsp. <it>equisimilis </it>(SDSE) causes invasive streptococcal infections, including streptococcal toxic shock syndrome (STSS), as does Lancefield group A <it>Streptococcus pyogenes </it>(GAS). We sequenced the entire genome of SDSE strain GGS_124 isolated from a patient with STSS.</p> <p>Results</p> <p>We found that GGS_124 consisted of a circular genome of 2,106,340 bp. Comparative analyses among bacterial genomes indicated that GGS_124 was most closely related to GAS. GGS_124 and GAS, but not other streptococci, shared a number of virulence factor genes, including genes encoding streptolysin O, NADase, and streptokinase A, distantly related to SIC (DRS), suggesting the importance of these factors in the development of invasive disease. GGS_124 contained 3 prophages, with one containing a virulence factor gene for streptodornase. All 3 prophages were significantly similar to GAS prophages that carry virulence factor genes, indicating that these prophages had transferred these genes between pathogens. SDSE was found to contain a gene encoding a superantigen, streptococcal exotoxin type G, but lacked several genes present in GAS that encode virulence factors, such as other superantigens, cysteine protease <it>speB</it>, and hyaluronan synthase operon <it>hasABC</it>. Similar to GGS_124, the SDSE strains contained larger numbers of clustered, regularly interspaced, short palindromic repeats (CRISPR) spacers than did GAS, suggesting that horizontal gene transfer via streptococcal phages between SDSE and GAS is somewhat restricted, although they share phage species.</p> <p>Conclusion</p> <p>Genome wide comparisons of SDSE with GAS indicate that SDSE is closely and quantitatively related to GAS. SDSE, however, lacks several virulence factors of GAS, including superantigens, SPE-B and the <it>hasABC </it>operon. CRISPR spacers may limit the horizontal transfer of phage encoded GAS virulence genes into SDSE. These findings may provide clues for dissecting the pathological roles of the virulence factors in SDSE and GAS that cause STSS.</p> |
| first_indexed | 2024-12-13T17:35:58Z |
| format | Article |
| id | doaj.art-4e8968874adc480e9c85e5f8b35ef499 |
| institution | Directory Open Access Journal |
| issn | 1471-2164 |
| language | English |
| last_indexed | 2024-12-13T17:35:58Z |
| publishDate | 2011-01-01 |
| publisher | BMC |
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| series | BMC Genomics |
| spelling | doaj.art-4e8968874adc480e9c85e5f8b35ef4992022-12-21T23:36:56ZengBMCBMC Genomics1471-21642011-01-011211710.1186/1471-2164-12-17Complete genome sequencing and analysis of a Lancefield group G <it>Streptococcus dysgalactiae </it>subsp. <it>equisimilis </it>strain causing streptococcal toxic shock syndrome (STSS)Ubukata KimikoYagi JunjiMurayama SomayOkumura KayoShimomura YumiKirikae TeruoMiyoshi-Akiyama Tohru<p>Abstract</p> <p>Background</p> <p><it>Streptococcus dysgalactiae </it>subsp. <it>equisimilis </it>(SDSE) causes invasive streptococcal infections, including streptococcal toxic shock syndrome (STSS), as does Lancefield group A <it>Streptococcus pyogenes </it>(GAS). We sequenced the entire genome of SDSE strain GGS_124 isolated from a patient with STSS.</p> <p>Results</p> <p>We found that GGS_124 consisted of a circular genome of 2,106,340 bp. Comparative analyses among bacterial genomes indicated that GGS_124 was most closely related to GAS. GGS_124 and GAS, but not other streptococci, shared a number of virulence factor genes, including genes encoding streptolysin O, NADase, and streptokinase A, distantly related to SIC (DRS), suggesting the importance of these factors in the development of invasive disease. GGS_124 contained 3 prophages, with one containing a virulence factor gene for streptodornase. All 3 prophages were significantly similar to GAS prophages that carry virulence factor genes, indicating that these prophages had transferred these genes between pathogens. SDSE was found to contain a gene encoding a superantigen, streptococcal exotoxin type G, but lacked several genes present in GAS that encode virulence factors, such as other superantigens, cysteine protease <it>speB</it>, and hyaluronan synthase operon <it>hasABC</it>. Similar to GGS_124, the SDSE strains contained larger numbers of clustered, regularly interspaced, short palindromic repeats (CRISPR) spacers than did GAS, suggesting that horizontal gene transfer via streptococcal phages between SDSE and GAS is somewhat restricted, although they share phage species.</p> <p>Conclusion</p> <p>Genome wide comparisons of SDSE with GAS indicate that SDSE is closely and quantitatively related to GAS. SDSE, however, lacks several virulence factors of GAS, including superantigens, SPE-B and the <it>hasABC </it>operon. CRISPR spacers may limit the horizontal transfer of phage encoded GAS virulence genes into SDSE. These findings may provide clues for dissecting the pathological roles of the virulence factors in SDSE and GAS that cause STSS.</p>http://www.biomedcentral.com/1471-2164/12/17 |
| spellingShingle | Ubukata Kimiko Yagi Junji Murayama Somay Okumura Kayo Shimomura Yumi Kirikae Teruo Miyoshi-Akiyama Tohru Complete genome sequencing and analysis of a Lancefield group G <it>Streptococcus dysgalactiae </it>subsp. <it>equisimilis </it>strain causing streptococcal toxic shock syndrome (STSS) BMC Genomics |
| title | Complete genome sequencing and analysis of a Lancefield group G <it>Streptococcus dysgalactiae </it>subsp. <it>equisimilis </it>strain causing streptococcal toxic shock syndrome (STSS) |
| title_full | Complete genome sequencing and analysis of a Lancefield group G <it>Streptococcus dysgalactiae </it>subsp. <it>equisimilis </it>strain causing streptococcal toxic shock syndrome (STSS) |
| title_fullStr | Complete genome sequencing and analysis of a Lancefield group G <it>Streptococcus dysgalactiae </it>subsp. <it>equisimilis </it>strain causing streptococcal toxic shock syndrome (STSS) |
| title_full_unstemmed | Complete genome sequencing and analysis of a Lancefield group G <it>Streptococcus dysgalactiae </it>subsp. <it>equisimilis </it>strain causing streptococcal toxic shock syndrome (STSS) |
| title_short | Complete genome sequencing and analysis of a Lancefield group G <it>Streptococcus dysgalactiae </it>subsp. <it>equisimilis </it>strain causing streptococcal toxic shock syndrome (STSS) |
| title_sort | complete genome sequencing and analysis of a lancefield group g it streptococcus dysgalactiae it subsp it equisimilis it strain causing streptococcal toxic shock syndrome stss |
| url | http://www.biomedcentral.com/1471-2164/12/17 |
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