Dynamical modelling of secondary metabolism and metabolic switches in Streptomyces xiamenensis 318
The production of secondary metabolites, while important for bioengineering purposes, presents a paradox in itself. Though widely existing in plants and bacteria, they have no definite physiological roles. Yet in both native habitats and laboratories, their production appears robust and follows appa...
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| Format: | Article |
| Language: | English |
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The Royal Society
2019-04-01
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| Series: | Royal Society Open Science |
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| Online Access: | https://royalsocietypublishing.org/doi/pdf/10.1098/rsos.190418 |
| _version_ | 1819079484998942720 |
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| author | Xiao-Mei Zhu Xing-Xing Zhang Run-Tan Cheng He-Lin Yu Ruo-Shi Yuan Xu-Liang Bu Jun Xu Ping Ao Yong-Cong Chen Min-Juan Xu |
| author_facet | Xiao-Mei Zhu Xing-Xing Zhang Run-Tan Cheng He-Lin Yu Ruo-Shi Yuan Xu-Liang Bu Jun Xu Ping Ao Yong-Cong Chen Min-Juan Xu |
| author_sort | Xiao-Mei Zhu |
| collection | DOAJ |
| description | The production of secondary metabolites, while important for bioengineering purposes, presents a paradox in itself. Though widely existing in plants and bacteria, they have no definite physiological roles. Yet in both native habitats and laboratories, their production appears robust and follows apparent metabolic switches. We show in this work that the enzyme-catalysed process may improve the metabolic stability of the cells. The latter can be responsible for the overall metabolic behaviours such as dynamic metabolic landscape, metabolic switches and robustness, which can in turn affect the genetic formation of the organism in question. Mangrove-derived Streptomyces xiamenensis 318, with a relatively compact genome for secondary metabolism, is used as a model organism in our investigation. Integrated studies via kinetic metabolic modelling, transcriptase measurements and metabolic profiling were performed on this strain. Our results demonstrate that the secondary metabolites increase the metabolic fitness of the organism via stabilizing the underlying metabolic network. And the fluxes directing to NADH, NADPH, acetyl-CoA and glutamate provide the key switches for the overall and secondary metabolism. The information may be helpful for improving the xiamenmycin production on the strain. |
| first_indexed | 2024-12-21T19:29:44Z |
| format | Article |
| id | doaj.art-4e8aca81fbed4e2ab8551472a4a987d8 |
| institution | Directory Open Access Journal |
| issn | 2054-5703 |
| language | English |
| last_indexed | 2024-12-21T19:29:44Z |
| publishDate | 2019-04-01 |
| publisher | The Royal Society |
| record_format | Article |
| series | Royal Society Open Science |
| spelling | doaj.art-4e8aca81fbed4e2ab8551472a4a987d82022-12-21T18:52:45ZengThe Royal SocietyRoyal Society Open Science2054-57032019-04-016410.1098/rsos.190418190418Dynamical modelling of secondary metabolism and metabolic switches in Streptomyces xiamenensis 318Xiao-Mei ZhuXing-Xing ZhangRun-Tan ChengHe-Lin YuRuo-Shi YuanXu-Liang BuJun XuPing AoYong-Cong ChenMin-Juan XuThe production of secondary metabolites, while important for bioengineering purposes, presents a paradox in itself. Though widely existing in plants and bacteria, they have no definite physiological roles. Yet in both native habitats and laboratories, their production appears robust and follows apparent metabolic switches. We show in this work that the enzyme-catalysed process may improve the metabolic stability of the cells. The latter can be responsible for the overall metabolic behaviours such as dynamic metabolic landscape, metabolic switches and robustness, which can in turn affect the genetic formation of the organism in question. Mangrove-derived Streptomyces xiamenensis 318, with a relatively compact genome for secondary metabolism, is used as a model organism in our investigation. Integrated studies via kinetic metabolic modelling, transcriptase measurements and metabolic profiling were performed on this strain. Our results demonstrate that the secondary metabolites increase the metabolic fitness of the organism via stabilizing the underlying metabolic network. And the fluxes directing to NADH, NADPH, acetyl-CoA and glutamate provide the key switches for the overall and secondary metabolism. The information may be helpful for improving the xiamenmycin production on the strain.https://royalsocietypublishing.org/doi/pdf/10.1098/rsos.190418secondary metabolismmetabolic switchmetabolic modellingdynamical landscapesystems biologystreptomyces |
| spellingShingle | Xiao-Mei Zhu Xing-Xing Zhang Run-Tan Cheng He-Lin Yu Ruo-Shi Yuan Xu-Liang Bu Jun Xu Ping Ao Yong-Cong Chen Min-Juan Xu Dynamical modelling of secondary metabolism and metabolic switches in Streptomyces xiamenensis 318 Royal Society Open Science secondary metabolism metabolic switch metabolic modelling dynamical landscape systems biology streptomyces |
| title | Dynamical modelling of secondary metabolism and metabolic switches in Streptomyces xiamenensis 318 |
| title_full | Dynamical modelling of secondary metabolism and metabolic switches in Streptomyces xiamenensis 318 |
| title_fullStr | Dynamical modelling of secondary metabolism and metabolic switches in Streptomyces xiamenensis 318 |
| title_full_unstemmed | Dynamical modelling of secondary metabolism and metabolic switches in Streptomyces xiamenensis 318 |
| title_short | Dynamical modelling of secondary metabolism and metabolic switches in Streptomyces xiamenensis 318 |
| title_sort | dynamical modelling of secondary metabolism and metabolic switches in streptomyces xiamenensis 318 |
| topic | secondary metabolism metabolic switch metabolic modelling dynamical landscape systems biology streptomyces |
| url | https://royalsocietypublishing.org/doi/pdf/10.1098/rsos.190418 |
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