Development of a real-time PCR assay for the detection of the golden mussel ( Limnoperna fortunei , Mytilidae) in environmental samples
ABSTRACT The golden mussel, Limnoperna fortunei, is among the most devastating invasive species in freshwater habitats worldwide, leading to severe environmental disturbances and economic losses. Therefore, management efforts would be greatly improved by methods that efficiently detect and quantify...
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| Format: | Article |
| Language: | English |
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Academia Brasileira de Ciências
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| Series: | Anais da Academia Brasileira de Ciências |
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| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652017000301041&lng=en&tlng=en |
| _version_ | 1828352332748816384 |
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| author | MARCIO R. PIE PATRÍCIA R. STRÖHER ANDRÉ O. AGOSTINIS RICARDO BELMONTE-LOPES MICHELLE Z. TADRA-SFEIR ANTONIO OSTRENSKY |
| author_facet | MARCIO R. PIE PATRÍCIA R. STRÖHER ANDRÉ O. AGOSTINIS RICARDO BELMONTE-LOPES MICHELLE Z. TADRA-SFEIR ANTONIO OSTRENSKY |
| author_sort | MARCIO R. PIE |
| collection | DOAJ |
| description | ABSTRACT The golden mussel, Limnoperna fortunei, is among the most devastating invasive species in freshwater habitats worldwide, leading to severe environmental disturbances and economic losses. Therefore, management efforts would be greatly improved by methods that efficiently detect and quantify the abundance of the golden mussel in freshwater habitats, particularly in early stages of colonization. In this study, we describe a highly-sensitive real-time PCR assay targeting a 100-bp region of the COI mitochondrial gene of the golden mussel. The method was able to detect as little as 0.225 pg of target DNA. This assay represents an important contribution to surveillance methods, as well as to optimize field measures to contain and manage populations of the golden mussel in its introduced range. |
| first_indexed | 2024-04-14T01:52:24Z |
| format | Article |
| id | doaj.art-4e8cc31ced5c48eab8dadd16cc8a5724 |
| institution | Directory Open Access Journal |
| issn | 1678-2690 |
| language | English |
| last_indexed | 2024-04-14T01:52:24Z |
| publisher | Academia Brasileira de Ciências |
| record_format | Article |
| series | Anais da Academia Brasileira de Ciências |
| spelling | doaj.art-4e8cc31ced5c48eab8dadd16cc8a57242022-12-22T02:19:16ZengAcademia Brasileira de CiênciasAnais da Academia Brasileira de Ciências1678-26908921041104510.1590/0001-3765201720160723S0001-37652017000301041Development of a real-time PCR assay for the detection of the golden mussel ( Limnoperna fortunei , Mytilidae) in environmental samplesMARCIO R. PIEPATRÍCIA R. STRÖHERANDRÉ O. AGOSTINISRICARDO BELMONTE-LOPESMICHELLE Z. TADRA-SFEIRANTONIO OSTRENSKYABSTRACT The golden mussel, Limnoperna fortunei, is among the most devastating invasive species in freshwater habitats worldwide, leading to severe environmental disturbances and economic losses. Therefore, management efforts would be greatly improved by methods that efficiently detect and quantify the abundance of the golden mussel in freshwater habitats, particularly in early stages of colonization. In this study, we describe a highly-sensitive real-time PCR assay targeting a 100-bp region of the COI mitochondrial gene of the golden mussel. The method was able to detect as little as 0.225 pg of target DNA. This assay represents an important contribution to surveillance methods, as well as to optimize field measures to contain and manage populations of the golden mussel in its introduced range.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652017000301041&lng=en&tlng=eneDNAinvasive speciesenvironmental DNAmolecular identification |
| spellingShingle | MARCIO R. PIE PATRÍCIA R. STRÖHER ANDRÉ O. AGOSTINIS RICARDO BELMONTE-LOPES MICHELLE Z. TADRA-SFEIR ANTONIO OSTRENSKY Development of a real-time PCR assay for the detection of the golden mussel ( Limnoperna fortunei , Mytilidae) in environmental samples Anais da Academia Brasileira de Ciências eDNA invasive species environmental DNA molecular identification |
| title | Development of a real-time PCR assay for the detection of the golden mussel ( Limnoperna fortunei , Mytilidae) in environmental samples |
| title_full | Development of a real-time PCR assay for the detection of the golden mussel ( Limnoperna fortunei , Mytilidae) in environmental samples |
| title_fullStr | Development of a real-time PCR assay for the detection of the golden mussel ( Limnoperna fortunei , Mytilidae) in environmental samples |
| title_full_unstemmed | Development of a real-time PCR assay for the detection of the golden mussel ( Limnoperna fortunei , Mytilidae) in environmental samples |
| title_short | Development of a real-time PCR assay for the detection of the golden mussel ( Limnoperna fortunei , Mytilidae) in environmental samples |
| title_sort | development of a real time pcr assay for the detection of the golden mussel limnoperna fortunei mytilidae in environmental samples |
| topic | eDNA invasive species environmental DNA molecular identification |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652017000301041&lng=en&tlng=en |
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