Neurotrophin-3 accelerates reendothelialization through inducing EPC mobilization and homing
Rapid endothelialization is an effective way to treat intimal hyperplasia after intravascular stent implantation. Blood vessels and nerves coordinate with each other in function, while neurotrophin-3 (NT-3) is an important class of nerve growth factors. Our study found that NT-3 promoted endothelial...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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De Gruyter
2020-05-01
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| Series: | Open Life Sciences |
| Subjects: | |
| Online Access: | https://doi.org/10.1515/biol-2020-0028 |
| _version_ | 1819016036907745280 |
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| author | Chen Yan Cao Jian Peng Weixia Chen Wen |
| author_facet | Chen Yan Cao Jian Peng Weixia Chen Wen |
| author_sort | Chen Yan |
| collection | DOAJ |
| description | Rapid endothelialization is an effective way to treat intimal hyperplasia after intravascular stent implantation. Blood vessels and nerves coordinate with each other in function, while neurotrophin-3 (NT-3) is an important class of nerve growth factors. Our study found that NT-3 promoted endothelial progenitor cell (EPC) mobilization, and the proportion of EPCs in peripheral blood was increased by 1.774 times compared with the control group. Besides, NT-3 promoted the expression of stromal cell-derived factor-1α (SDF-1α), matrix metalloproteinase-9 (MMP9), and chemokine (C-X-C motif) receptor 4 (CXCR4) in EPCs, which increased by 59.89%, 74.46%, and 107.7%, respectively, compared with the control group. Transwell experiments showed that NT-3 enhanced the migration of EPCs by 1.31 times. Flow chamber experiments demonstrated that NT-3 captured more circulating EPCs. As shown by ELISA results, NT-3 can promote the paracrine of vascular endothelial growth factor, interleukin-8, MMP-9, and SDF-1 from EPCs. Such increased angiogenic growth factors further accelerated the closure of endothelial cell scratches. Additionally, EPC-conditioned medium in the NT-3 group significantly inhibited the proliferation of vascular smooth muscle cells. Then animal experiments also illustrated that NT-3 prominently accelerated the endothelialization of injured carotid artery. In short, NT-3 accelerated rapid reendothelialization of injured carotid artery through promoting EPC mobilization and homing. |
| first_indexed | 2024-12-21T02:41:15Z |
| format | Article |
| id | doaj.art-4e8f93dd2c0646c59f1ab2ef7935d559 |
| institution | Directory Open Access Journal |
| issn | 2391-5412 |
| language | English |
| last_indexed | 2024-12-21T02:41:15Z |
| publishDate | 2020-05-01 |
| publisher | De Gruyter |
| record_format | Article |
| series | Open Life Sciences |
| spelling | doaj.art-4e8f93dd2c0646c59f1ab2ef7935d5592022-12-21T19:18:39ZengDe GruyterOpen Life Sciences2391-54122020-05-0115124125010.1515/biol-2020-0028biol-2020-0028Neurotrophin-3 accelerates reendothelialization through inducing EPC mobilization and homingChen Yan0Cao Jian1Peng Weixia2Chen Wen3Department of Integrated TCM & Western Medicine, Central Hospital of Yiyang city, YiyangHunan, 413200, ChinaDepartment of General Surgery, Central Hospital of Yiyang city, Yiyang, Hunan, 413200, ChinaDepartment of Integrated TCM & Western Medicine, Central Hospital of Yiyang city, YiyangHunan, 413200, ChinaThe 8th Medical Center of Chinese PLA General Hospital, Beijing, 100091, ChinaRapid endothelialization is an effective way to treat intimal hyperplasia after intravascular stent implantation. Blood vessels and nerves coordinate with each other in function, while neurotrophin-3 (NT-3) is an important class of nerve growth factors. Our study found that NT-3 promoted endothelial progenitor cell (EPC) mobilization, and the proportion of EPCs in peripheral blood was increased by 1.774 times compared with the control group. Besides, NT-3 promoted the expression of stromal cell-derived factor-1α (SDF-1α), matrix metalloproteinase-9 (MMP9), and chemokine (C-X-C motif) receptor 4 (CXCR4) in EPCs, which increased by 59.89%, 74.46%, and 107.7%, respectively, compared with the control group. Transwell experiments showed that NT-3 enhanced the migration of EPCs by 1.31 times. Flow chamber experiments demonstrated that NT-3 captured more circulating EPCs. As shown by ELISA results, NT-3 can promote the paracrine of vascular endothelial growth factor, interleukin-8, MMP-9, and SDF-1 from EPCs. Such increased angiogenic growth factors further accelerated the closure of endothelial cell scratches. Additionally, EPC-conditioned medium in the NT-3 group significantly inhibited the proliferation of vascular smooth muscle cells. Then animal experiments also illustrated that NT-3 prominently accelerated the endothelialization of injured carotid artery. In short, NT-3 accelerated rapid reendothelialization of injured carotid artery through promoting EPC mobilization and homing.https://doi.org/10.1515/biol-2020-0028neurotrophin-3endothelializationendothelial progenitor cellsintravascular stent |
| spellingShingle | Chen Yan Cao Jian Peng Weixia Chen Wen Neurotrophin-3 accelerates reendothelialization through inducing EPC mobilization and homing Open Life Sciences neurotrophin-3 endothelialization endothelial progenitor cells intravascular stent |
| title | Neurotrophin-3 accelerates reendothelialization through inducing EPC mobilization and homing |
| title_full | Neurotrophin-3 accelerates reendothelialization through inducing EPC mobilization and homing |
| title_fullStr | Neurotrophin-3 accelerates reendothelialization through inducing EPC mobilization and homing |
| title_full_unstemmed | Neurotrophin-3 accelerates reendothelialization through inducing EPC mobilization and homing |
| title_short | Neurotrophin-3 accelerates reendothelialization through inducing EPC mobilization and homing |
| title_sort | neurotrophin 3 accelerates reendothelialization through inducing epc mobilization and homing |
| topic | neurotrophin-3 endothelialization endothelial progenitor cells intravascular stent |
| url | https://doi.org/10.1515/biol-2020-0028 |
| work_keys_str_mv | AT chenyan neurotrophin3acceleratesreendothelializationthroughinducingepcmobilizationandhoming AT caojian neurotrophin3acceleratesreendothelializationthroughinducingepcmobilizationandhoming AT pengweixia neurotrophin3acceleratesreendothelializationthroughinducingepcmobilizationandhoming AT chenwen neurotrophin3acceleratesreendothelializationthroughinducingepcmobilizationandhoming |