Genome sequence and characterization of Streptomyces phages Vanseggelen and Verabelle, representing two new species within the genus Camvirus
Abstract Despite the rising interest in bacteriophages, little is known about their infection cycle and lifestyle in a multicellular host. Even in the model system Streptomyces, only a small number of phages have been sequenced and well characterized so far. Here, we report the complete characteriza...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2023-11-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-023-47634-3 |
| _version_ | 1797559537248501760 |
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| author | Véronique Ongenae Annabel Kempff Vera van Neer Helena Shomar Florian Tesson Daniël Rozen Ariane Briegel Dennis Claessen |
| author_facet | Véronique Ongenae Annabel Kempff Vera van Neer Helena Shomar Florian Tesson Daniël Rozen Ariane Briegel Dennis Claessen |
| author_sort | Véronique Ongenae |
| collection | DOAJ |
| description | Abstract Despite the rising interest in bacteriophages, little is known about their infection cycle and lifestyle in a multicellular host. Even in the model system Streptomyces, only a small number of phages have been sequenced and well characterized so far. Here, we report the complete characterization and genome sequences of Streptomyces phages Vanseggelen and Verabelle isolated using Streptomyces coelicolor as a host. A wide range of Streptomyces strains could be infected by both phages, but neither of the two phages was able to infect members of the closely related sister genus Kitasatospora. The phages Vanseggelen and Verabelle have a double-stranded DNA genome with lengths of 48,720 and 48,126 bp, respectively. Both phage genomes contain 72 putative genes, and the presence of an integrase encoding protein indicates a lysogenic lifestyle. Characterization of the phages revealed their stability over a wide range of temperatures (30–45 °C) and pH values (4–10). In conclusion, Streptomyces phage Vanseggelen and Streptomyces phage Verabelle are newly isolated phages that can be classified as new species in the genus Camvirus, within the subfamily Arquattrovirinae. |
| first_indexed | 2024-03-10T17:46:48Z |
| format | Article |
| id | doaj.art-4e91e8285a2f45a1ae5dbda6fad62980 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-03-10T17:46:48Z |
| publishDate | 2023-11-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-4e91e8285a2f45a1ae5dbda6fad629802023-11-20T09:30:07ZengNature PortfolioScientific Reports2045-23222023-11-0113111010.1038/s41598-023-47634-3Genome sequence and characterization of Streptomyces phages Vanseggelen and Verabelle, representing two new species within the genus CamvirusVéronique Ongenae0Annabel Kempff1Vera van Neer2Helena Shomar3Florian Tesson4Daniël Rozen5Ariane Briegel6Dennis Claessen7Molecular Biotechnology, Institute of Biology, Leiden UniversityMolecular Biotechnology, Institute of Biology, Leiden UniversityMolecular Biotechnology, Institute of Biology, Leiden UniversityMDM Lab, Department Genomes and Genetics, Pasteur InstituteINSERM, U1284, Université Paris-CitéMolecular Biotechnology, Institute of Biology, Leiden UniversityMolecular Biotechnology, Institute of Biology, Leiden UniversityMolecular Biotechnology, Institute of Biology, Leiden UniversityAbstract Despite the rising interest in bacteriophages, little is known about their infection cycle and lifestyle in a multicellular host. Even in the model system Streptomyces, only a small number of phages have been sequenced and well characterized so far. Here, we report the complete characterization and genome sequences of Streptomyces phages Vanseggelen and Verabelle isolated using Streptomyces coelicolor as a host. A wide range of Streptomyces strains could be infected by both phages, but neither of the two phages was able to infect members of the closely related sister genus Kitasatospora. The phages Vanseggelen and Verabelle have a double-stranded DNA genome with lengths of 48,720 and 48,126 bp, respectively. Both phage genomes contain 72 putative genes, and the presence of an integrase encoding protein indicates a lysogenic lifestyle. Characterization of the phages revealed their stability over a wide range of temperatures (30–45 °C) and pH values (4–10). In conclusion, Streptomyces phage Vanseggelen and Streptomyces phage Verabelle are newly isolated phages that can be classified as new species in the genus Camvirus, within the subfamily Arquattrovirinae.https://doi.org/10.1038/s41598-023-47634-3 |
| spellingShingle | Véronique Ongenae Annabel Kempff Vera van Neer Helena Shomar Florian Tesson Daniël Rozen Ariane Briegel Dennis Claessen Genome sequence and characterization of Streptomyces phages Vanseggelen and Verabelle, representing two new species within the genus Camvirus Scientific Reports |
| title | Genome sequence and characterization of Streptomyces phages Vanseggelen and Verabelle, representing two new species within the genus Camvirus |
| title_full | Genome sequence and characterization of Streptomyces phages Vanseggelen and Verabelle, representing two new species within the genus Camvirus |
| title_fullStr | Genome sequence and characterization of Streptomyces phages Vanseggelen and Verabelle, representing two new species within the genus Camvirus |
| title_full_unstemmed | Genome sequence and characterization of Streptomyces phages Vanseggelen and Verabelle, representing two new species within the genus Camvirus |
| title_short | Genome sequence and characterization of Streptomyces phages Vanseggelen and Verabelle, representing two new species within the genus Camvirus |
| title_sort | genome sequence and characterization of streptomyces phages vanseggelen and verabelle representing two new species within the genus camvirus |
| url | https://doi.org/10.1038/s41598-023-47634-3 |
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