A comparative study of the effect of different topical agents on burn wound infections
Background: Topical agents are used to treat burn wound infections. Aims and Objective: The present work was aimed to find out the in vitro efficacy of different topical agents against burn wound pathogens. Settings and Design: Randomly selected gram-positive (29) and gram-negative bacterial (119) i...
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| Format: | Article |
| Language: | English |
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Thieme Medical Publishers, Inc.
2012-05-01
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| Series: | Indian Journal of Plastic Surgery |
| Subjects: | |
| Online Access: | http://www.thieme-connect.de/DOI/DOI?10.4103/0970-0358.101320 |
| _version_ | 1831676760623677440 |
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| author | Katara Gunjan Chamania Shobha Chitnis Sheetal Hemvani Nanda Chitnis Vikrant Dhananjay Sadashiv Chitnis |
| author_facet | Katara Gunjan Chamania Shobha Chitnis Sheetal Hemvani Nanda Chitnis Vikrant Dhananjay Sadashiv Chitnis |
| author_sort | Katara Gunjan |
| collection | DOAJ |
| description | Background: Topical agents are used to treat burn wound infections. Aims and Objective: The present work was aimed to find out the in vitro efficacy of different topical agents against burn wound pathogens. Settings and Design: Randomly selected gram-positive (29) and gram-negative bacterial (119) isolates from burn wound cases admitted in burn unit of Choithram Hospital and Research Centre, Indore, were included in the in vitro activity testing for silver nitrate, silver sulphadiazine (SSD), chlorhexidine, cetrimide, nitrofuran, soframycin, betadine, benzalkonium chloride and honey by growth inhibition on agar medium. Materials and Methods: Multidrug-resistant isolates of gram-positive and gram-negative bacteria were checked for different topical agents. 1% topical agent was mixed with Mueller-Hinton agar. Two microlitres of bacterial suspension adjusted to 0.5 McFarland turbidity standard was spread over the topical agent containing plates. The plates without the topical agent were used as control plates. The plates were incubated for 48 h at 37C. Results: SSD (148/148), silver nitrate (148/148) and chlorhexidine (148/148) showed excellent activity against all the pathogens. Neosporin had poor activity against Pseudomonas aeruginosa, (4/44) Proteus spp. (2/4) and group D streptococci (1/4). Betadine did not show activity against the bacterial isolates in the presence of organic matter. Honey did not exert any antimicrobial activity under the study conditions. Conclusion: SSD, silver nitrate and chlorhexidine have excellent activity against all the bacterial pathogens and could be used empirically, while identification of the infective agent is required for selecting the alternative topical agents such as nitrofuran, soframycin, and benzalkonium chloride. |
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| format | Article |
| id | doaj.art-4e91f5d4421041a084a8eb856290cc9d |
| institution | Directory Open Access Journal |
| issn | 0970-0358 1998-376X |
| language | English |
| last_indexed | 2024-12-20T04:28:59Z |
| publishDate | 2012-05-01 |
| publisher | Thieme Medical Publishers, Inc. |
| record_format | Article |
| series | Indian Journal of Plastic Surgery |
| spelling | doaj.art-4e91f5d4421041a084a8eb856290cc9d2022-12-21T19:53:26ZengThieme Medical Publishers, Inc.Indian Journal of Plastic Surgery0970-03581998-376X2012-05-01450237437810.4103/0970-0358.101320A comparative study of the effect of different topical agents on burn wound infectionsKatara Gunjan0Chamania Shobha1Chitnis Sheetal2Hemvani Nanda3Chitnis Vikrant4Dhananjay Sadashiv Chitnis5Department of Microbiology, Immunology and Molecular Biology, Choithram Hospital and Research Centre, Manik Bagh Road, Indore, Madhya Pradesh, IndiaDepartment of Burn Surgery, Choithram Hospital and Research Centre, Manik Bagh Road, Indore, Madhya Pradesh, IndiaDepartment of Microbiology, Immunology and Molecular Biology, Choithram Hospital and Research Centre, Manik Bagh Road, Indore, Madhya Pradesh, IndiaDepartment of Microbiology, Immunology and Molecular Biology, Choithram Hospital and Research Centre, Manik Bagh Road, Indore, Madhya Pradesh, IndiaDepartment of Microbiology and Immunology, Aurobindo Medical College, Sanwer Road, Indore, Madhya Pradesh, IndiaDepartment of Microbiology, Immunology and Molecular Biology, Choithram Hospital and Research Centre, Manik Bagh Road, Indore, Madhya Pradesh, IndiaBackground: Topical agents are used to treat burn wound infections. Aims and Objective: The present work was aimed to find out the in vitro efficacy of different topical agents against burn wound pathogens. Settings and Design: Randomly selected gram-positive (29) and gram-negative bacterial (119) isolates from burn wound cases admitted in burn unit of Choithram Hospital and Research Centre, Indore, were included in the in vitro activity testing for silver nitrate, silver sulphadiazine (SSD), chlorhexidine, cetrimide, nitrofuran, soframycin, betadine, benzalkonium chloride and honey by growth inhibition on agar medium. Materials and Methods: Multidrug-resistant isolates of gram-positive and gram-negative bacteria were checked for different topical agents. 1% topical agent was mixed with Mueller-Hinton agar. Two microlitres of bacterial suspension adjusted to 0.5 McFarland turbidity standard was spread over the topical agent containing plates. The plates without the topical agent were used as control plates. The plates were incubated for 48 h at 37C. Results: SSD (148/148), silver nitrate (148/148) and chlorhexidine (148/148) showed excellent activity against all the pathogens. Neosporin had poor activity against Pseudomonas aeruginosa, (4/44) Proteus spp. (2/4) and group D streptococci (1/4). Betadine did not show activity against the bacterial isolates in the presence of organic matter. Honey did not exert any antimicrobial activity under the study conditions. Conclusion: SSD, silver nitrate and chlorhexidine have excellent activity against all the bacterial pathogens and could be used empirically, while identification of the infective agent is required for selecting the alternative topical agents such as nitrofuran, soframycin, and benzalkonium chloride.http://www.thieme-connect.de/DOI/DOI?10.4103/0970-0358.101320benzalkonium chlorideburn woundscetrimidechlorhexidinenitrofuransilver nitratesilver sulphadiazinetopical gents |
| spellingShingle | Katara Gunjan Chamania Shobha Chitnis Sheetal Hemvani Nanda Chitnis Vikrant Dhananjay Sadashiv Chitnis A comparative study of the effect of different topical agents on burn wound infections Indian Journal of Plastic Surgery benzalkonium chloride burn wounds cetrimide chlorhexidine nitrofuran silver nitrate silver sulphadiazine topical gents |
| title | A comparative study of the effect of different topical agents on burn wound infections |
| title_full | A comparative study of the effect of different topical agents on burn wound infections |
| title_fullStr | A comparative study of the effect of different topical agents on burn wound infections |
| title_full_unstemmed | A comparative study of the effect of different topical agents on burn wound infections |
| title_short | A comparative study of the effect of different topical agents on burn wound infections |
| title_sort | comparative study of the effect of different topical agents on burn wound infections |
| topic | benzalkonium chloride burn wounds cetrimide chlorhexidine nitrofuran silver nitrate silver sulphadiazine topical gents |
| url | http://www.thieme-connect.de/DOI/DOI?10.4103/0970-0358.101320 |
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