Evaluation of the Panbio COVID-19 Antigen Rapid Diagnostic Test in Subjects Infected with Omicron Using Different Specimens
ABSTRACT Community testing is a crucial tool for the early identification of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection and transmission control. The emergence of the highly mutated Omicron variant (B.1.1.529) raised concerns about its primary site of replication, impacti...
| Main Authors: | , , , , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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American Society for Microbiology
2022-06-01
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| Series: | Microbiology Spectrum |
| Subjects: | |
| Online Access: | https://journals.asm.org/doi/10.1128/spectrum.01250-22 |
| _version_ | 1818218422708731904 |
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| author | Rafael Mello Galliez Larissa Bomfim Diana Mariani Isabela de Carvalho Leitão Anna Carla Pinto Castiñeiras Cassia Cristina Alves Gonçalves Bianca Ortiz da Silva Pedro Henrique Cardoso Monica Barcelos Arruda Patricia Alvarez Rodrigo Brindeiro Victor Akira Ota Débora Gomes Marins Rodrigues Luciana Jesus da Costa Orlando da Costa Ferreira Terezinha Marta Pereira Pinto Castiñeiras Debora Souza Faffe Amilcar Tanuri |
| author_facet | Rafael Mello Galliez Larissa Bomfim Diana Mariani Isabela de Carvalho Leitão Anna Carla Pinto Castiñeiras Cassia Cristina Alves Gonçalves Bianca Ortiz da Silva Pedro Henrique Cardoso Monica Barcelos Arruda Patricia Alvarez Rodrigo Brindeiro Victor Akira Ota Débora Gomes Marins Rodrigues Luciana Jesus da Costa Orlando da Costa Ferreira Terezinha Marta Pereira Pinto Castiñeiras Debora Souza Faffe Amilcar Tanuri |
| author_sort | Rafael Mello Galliez |
| collection | DOAJ |
| description | ABSTRACT Community testing is a crucial tool for the early identification of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection and transmission control. The emergence of the highly mutated Omicron variant (B.1.1.529) raised concerns about its primary site of replication, impacting sample collection and its detectability by rapid antigen tests. We tested the performance of the Panbio antigen rapid diagnostic test (Ag-RDT) using nasal and oral specimens for COVID-19 diagnosis in 192 symptomatic individuals, with quantitative reverse transcription-PCR (RT-qPCR) of nasopharyngeal samples as a control. Variant of concern (VOC) investigation was performed with the 4Plex SARS-CoV-2 screening kit. The SARS-CoV-2 positivity rate was 66.2%, with 99% of the positive samples showing an amplification profile consistent with that of the Omicron variant. Nasal Ag-RDT showed higher sensitivity (89%) than oral (12.6%) Ag-RDT. Our data showed good performance of the Ag-RDT in a pandemic scenario dominated by the Omicron VOC. Furthermore, our data also demonstrated that the Panbio COVID-19 antigen rapid diagnostic test does not provide good sensitivity with oral swabs for Omicron Ag-RDT detection. IMPORTANCE This study showed that the antigen rapid test for COVID19 worked fine using nasal swabs when it was utilized in patients infected with the Omicron variant, showing a concordance with PCR in 93% of patients tested. The nasal swab yielded more reliable results than the oral swab when an antigen rapid diagnosis test (the Panbio COVID-19 antigen rapid diagnostic test) was used in patients infected with the Omicron variant. |
| first_indexed | 2024-12-12T07:23:31Z |
| format | Article |
| id | doaj.art-4e960b00dadb49e3b8be83fbc1ebe0fd |
| institution | Directory Open Access Journal |
| issn | 2165-0497 |
| language | English |
| last_indexed | 2024-12-12T07:23:31Z |
| publishDate | 2022-06-01 |
| publisher | American Society for Microbiology |
| record_format | Article |
| series | Microbiology Spectrum |
| spelling | doaj.art-4e960b00dadb49e3b8be83fbc1ebe0fd2022-12-22T00:33:12ZengAmerican Society for MicrobiologyMicrobiology Spectrum2165-04972022-06-0110310.1128/spectrum.01250-22Evaluation of the Panbio COVID-19 Antigen Rapid Diagnostic Test in Subjects Infected with Omicron Using Different SpecimensRafael Mello Galliez0Larissa Bomfim1Diana Mariani2Isabela de Carvalho Leitão3Anna Carla Pinto Castiñeiras4Cassia Cristina Alves Gonçalves5Bianca Ortiz da Silva6Pedro Henrique Cardoso7Monica Barcelos Arruda8Patricia Alvarez9Rodrigo Brindeiro10Victor Akira Ota11Débora Gomes Marins Rodrigues12Luciana Jesus da Costa13Orlando da Costa Ferreira14Terezinha Marta Pereira Pinto Castiñeiras15Debora Souza Faffe16Amilcar Tanuri17Núcleo de Enfrentamento e Estudos de Doenças Infecciosas Emergentes e Reemergentes, Universidade Federal do Rio de Janeiro, Rio de Janeiro, BrazilLaboratório de Virologia Molecular, Departamento de Genética, Instituto de Biologia, Universidade Federal do Rio de Janeiro, Rio de Janeiro, BrazilLaboratório de Virologia Molecular, Departamento de Genética, Instituto de Biologia, Universidade Federal do Rio de Janeiro, Rio de Janeiro, BrazilNúcleo de Enfrentamento e Estudos de Doenças Infecciosas Emergentes e Reemergentes, Universidade Federal do Rio de Janeiro, Rio de Janeiro, BrazilNúcleo de Enfrentamento e Estudos de Doenças Infecciosas Emergentes e Reemergentes, Universidade Federal do Rio de Janeiro, Rio de Janeiro, BrazilLaboratório de Virologia Molecular, Departamento de Genética, Instituto de Biologia, Universidade Federal do Rio de Janeiro, Rio de Janeiro, BrazilNúcleo de Enfrentamento e Estudos de Doenças Infecciosas Emergentes e Reemergentes, Universidade Federal do Rio de Janeiro, Rio de Janeiro, BrazilInstituto de Tecnologia de Imunobiológicos Bio-manguinhos, Fundação Oswaldo Cruz/Fiocruz, Rio de Janeiro, BrazilInstituto de Tecnologia de Imunobiológicos Bio-manguinhos, Fundação Oswaldo Cruz/Fiocruz, Rio de Janeiro, BrazilInstituto de Tecnologia de Imunobiológicos Bio-manguinhos, Fundação Oswaldo Cruz/Fiocruz, Rio de Janeiro, BrazilLaboratório de Virologia Molecular, Departamento de Genética, Instituto de Biologia, Universidade Federal do Rio de Janeiro, Rio de Janeiro, BrazilNúcleo de Enfrentamento e Estudos de Doenças Infecciosas Emergentes e Reemergentes, Universidade Federal do Rio de Janeiro, Rio de Janeiro, BrazilNúcleo de Enfrentamento e Estudos de Doenças Infecciosas Emergentes e Reemergentes, Universidade Federal do Rio de Janeiro, Rio de Janeiro, BrazilNúcleo de Enfrentamento e Estudos de Doenças Infecciosas Emergentes e Reemergentes, Universidade Federal do Rio de Janeiro, Rio de Janeiro, BrazilNúcleo de Enfrentamento e Estudos de Doenças Infecciosas Emergentes e Reemergentes, Universidade Federal do Rio de Janeiro, Rio de Janeiro, BrazilNúcleo de Enfrentamento e Estudos de Doenças Infecciosas Emergentes e Reemergentes, Universidade Federal do Rio de Janeiro, Rio de Janeiro, BrazilNúcleo de Enfrentamento e Estudos de Doenças Infecciosas Emergentes e Reemergentes, Universidade Federal do Rio de Janeiro, Rio de Janeiro, BrazilNúcleo de Enfrentamento e Estudos de Doenças Infecciosas Emergentes e Reemergentes, Universidade Federal do Rio de Janeiro, Rio de Janeiro, BrazilABSTRACT Community testing is a crucial tool for the early identification of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection and transmission control. The emergence of the highly mutated Omicron variant (B.1.1.529) raised concerns about its primary site of replication, impacting sample collection and its detectability by rapid antigen tests. We tested the performance of the Panbio antigen rapid diagnostic test (Ag-RDT) using nasal and oral specimens for COVID-19 diagnosis in 192 symptomatic individuals, with quantitative reverse transcription-PCR (RT-qPCR) of nasopharyngeal samples as a control. Variant of concern (VOC) investigation was performed with the 4Plex SARS-CoV-2 screening kit. The SARS-CoV-2 positivity rate was 66.2%, with 99% of the positive samples showing an amplification profile consistent with that of the Omicron variant. Nasal Ag-RDT showed higher sensitivity (89%) than oral (12.6%) Ag-RDT. Our data showed good performance of the Ag-RDT in a pandemic scenario dominated by the Omicron VOC. Furthermore, our data also demonstrated that the Panbio COVID-19 antigen rapid diagnostic test does not provide good sensitivity with oral swabs for Omicron Ag-RDT detection. IMPORTANCE This study showed that the antigen rapid test for COVID19 worked fine using nasal swabs when it was utilized in patients infected with the Omicron variant, showing a concordance with PCR in 93% of patients tested. The nasal swab yielded more reliable results than the oral swab when an antigen rapid diagnosis test (the Panbio COVID-19 antigen rapid diagnostic test) was used in patients infected with the Omicron variant.https://journals.asm.org/doi/10.1128/spectrum.01250-22Ag-RDTOmicronrapid test |
| spellingShingle | Rafael Mello Galliez Larissa Bomfim Diana Mariani Isabela de Carvalho Leitão Anna Carla Pinto Castiñeiras Cassia Cristina Alves Gonçalves Bianca Ortiz da Silva Pedro Henrique Cardoso Monica Barcelos Arruda Patricia Alvarez Rodrigo Brindeiro Victor Akira Ota Débora Gomes Marins Rodrigues Luciana Jesus da Costa Orlando da Costa Ferreira Terezinha Marta Pereira Pinto Castiñeiras Debora Souza Faffe Amilcar Tanuri Evaluation of the Panbio COVID-19 Antigen Rapid Diagnostic Test in Subjects Infected with Omicron Using Different Specimens Microbiology Spectrum Ag-RDT Omicron rapid test |
| title | Evaluation of the Panbio COVID-19 Antigen Rapid Diagnostic Test in Subjects Infected with Omicron Using Different Specimens |
| title_full | Evaluation of the Panbio COVID-19 Antigen Rapid Diagnostic Test in Subjects Infected with Omicron Using Different Specimens |
| title_fullStr | Evaluation of the Panbio COVID-19 Antigen Rapid Diagnostic Test in Subjects Infected with Omicron Using Different Specimens |
| title_full_unstemmed | Evaluation of the Panbio COVID-19 Antigen Rapid Diagnostic Test in Subjects Infected with Omicron Using Different Specimens |
| title_short | Evaluation of the Panbio COVID-19 Antigen Rapid Diagnostic Test in Subjects Infected with Omicron Using Different Specimens |
| title_sort | evaluation of the panbio covid 19 antigen rapid diagnostic test in subjects infected with omicron using different specimens |
| topic | Ag-RDT Omicron rapid test |
| url | https://journals.asm.org/doi/10.1128/spectrum.01250-22 |
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