Trans-cinnamaldehyde nanoemulsion wash inactivates Salmonella Enteritidis on shelled eggs without affecting egg color
ABSTRACT: Salmonella Enteritidis is a major foodborne pathogen that causes enteric illnesses in humans, primarily through the consumption of contaminated poultry meat and eggs. Despite implementation of traditional disinfection approaches to reduce S. Enteritidis contamination, egg-borne outbreaks c...
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| Format: | Article |
| Language: | English |
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Elsevier
2023-04-01
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| Series: | Poultry Science |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S0032579123000470 |
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| author | J. Allen B. Balasubramanian K. Rankin T. Shah A.M. Donoghue I. Upadhyaya B. Sartini Y. Luo A. Upadhyay |
| author_facet | J. Allen B. Balasubramanian K. Rankin T. Shah A.M. Donoghue I. Upadhyaya B. Sartini Y. Luo A. Upadhyay |
| author_sort | J. Allen |
| collection | DOAJ |
| description | ABSTRACT: Salmonella Enteritidis is a major foodborne pathogen that causes enteric illnesses in humans, primarily through the consumption of contaminated poultry meat and eggs. Despite implementation of traditional disinfection approaches to reduce S. Enteritidis contamination, egg-borne outbreaks continue to occur, raising public health concerns and adversely affecting the popularity and profitability for the poultry industry. Generally Recognized as Safe (GRAS) status phytochemicals such as Trans-cinnamaldehyde (TC) have previously shown to exhibit anti-Salmonella efficacy, however, the low solubility of TC is a major hurdle in its adoption as an egg wash treatment. Therefore, the present study investigated the efficacy of Trans-cinnamaldehyde nanoemulsions (TCNE) prepared with emulsifiers Tween 80 (Tw.80) or Gum Arabic and lecithin (GAL) as dip treatments, at 34°C, for reducing S. Enteritidis on shelled eggs in presence or absence of 5% chicken litter. In addition, the efficacy of TCNE dip treatments in reducing trans-shell migration of S. Enteritidis across shell barrier was investigated. The effect of wash treatments on shell color were evaluated on d 0, 1, 7, and 14 of refrigerated storage. TCNE-Tw.80 or GAL treatments (0.06, 0.12, 0.24, 0.48%) were effective in inactivating S. Enteritidis by at least 2 to 2.5 log cfu/egg as early as 1 min of washing time (P < 0.05). In presence of organic matter, nanoemulsions (0.48%) reduced S. Enteritidis counts by ∼ 2 to 2.5 log cfu/egg as early as 1 min, (P < 0.05). Nanoemulsion wash also inhibited trans-shell migration of S. Enteritidis, as compared to control (P < 0.05). The nanoemulsion wash treatments did not affect shell color (P > 0.05). Results suggest that TCNE could potentially be used as an antimicrobial wash to reduce S. Enteritidis on shelled eggs, although further studies investigating the effect of TCNE wash treatments on organoleptic properties of eggs are necessary. |
| first_indexed | 2024-04-09T20:05:15Z |
| format | Article |
| id | doaj.art-4e9cccaf5f634406a145b802137604bd |
| institution | Directory Open Access Journal |
| issn | 0032-5791 |
| language | English |
| last_indexed | 2024-04-09T20:05:15Z |
| publishDate | 2023-04-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Poultry Science |
| spelling | doaj.art-4e9cccaf5f634406a145b802137604bd2023-04-02T06:10:52ZengElsevierPoultry Science0032-57912023-04-011024102523Trans-cinnamaldehyde nanoemulsion wash inactivates Salmonella Enteritidis on shelled eggs without affecting egg colorJ. Allen0B. Balasubramanian1K. Rankin2T. Shah3A.M. Donoghue4I. Upadhyaya5B. Sartini6Y. Luo7A. Upadhyay8University of Connecticut, Department of Animal Science, Storrs, CT 06269, USAUniversity of Connecticut, Department of Animal Science, Storrs, CT 06269, USAUniversity of Connecticut, Department of Animal Science, Storrs, CT 06269, USAUniversity of Connecticut, Department of Animal Science, Storrs, CT 06269, USAUSDA-ARS, Poultry Production and Product Safety Research, Fayetteville, AR 72701, USAUniversity of Connecticut, Department of Extension, Storrs, CT 06269, USAUniversity of Rhode Island, Department of Fisheries, Animal & Veterinary Science, Kingston, RI 02881, USAUniversity of Connecticut, Department of Nutritional Sciences, Storrs, CT 06269, USAUniversity of Connecticut, Department of Animal Science, Storrs, CT 06269, USA; Corresponding author:ABSTRACT: Salmonella Enteritidis is a major foodborne pathogen that causes enteric illnesses in humans, primarily through the consumption of contaminated poultry meat and eggs. Despite implementation of traditional disinfection approaches to reduce S. Enteritidis contamination, egg-borne outbreaks continue to occur, raising public health concerns and adversely affecting the popularity and profitability for the poultry industry. Generally Recognized as Safe (GRAS) status phytochemicals such as Trans-cinnamaldehyde (TC) have previously shown to exhibit anti-Salmonella efficacy, however, the low solubility of TC is a major hurdle in its adoption as an egg wash treatment. Therefore, the present study investigated the efficacy of Trans-cinnamaldehyde nanoemulsions (TCNE) prepared with emulsifiers Tween 80 (Tw.80) or Gum Arabic and lecithin (GAL) as dip treatments, at 34°C, for reducing S. Enteritidis on shelled eggs in presence or absence of 5% chicken litter. In addition, the efficacy of TCNE dip treatments in reducing trans-shell migration of S. Enteritidis across shell barrier was investigated. The effect of wash treatments on shell color were evaluated on d 0, 1, 7, and 14 of refrigerated storage. TCNE-Tw.80 or GAL treatments (0.06, 0.12, 0.24, 0.48%) were effective in inactivating S. Enteritidis by at least 2 to 2.5 log cfu/egg as early as 1 min of washing time (P < 0.05). In presence of organic matter, nanoemulsions (0.48%) reduced S. Enteritidis counts by ∼ 2 to 2.5 log cfu/egg as early as 1 min, (P < 0.05). Nanoemulsion wash also inhibited trans-shell migration of S. Enteritidis, as compared to control (P < 0.05). The nanoemulsion wash treatments did not affect shell color (P > 0.05). Results suggest that TCNE could potentially be used as an antimicrobial wash to reduce S. Enteritidis on shelled eggs, although further studies investigating the effect of TCNE wash treatments on organoleptic properties of eggs are necessary.http://www.sciencedirect.com/science/article/pii/S0032579123000470trans-cinnamaldehydesalmonella enteritidisnanoemulsionfood-grade emulsifieregg |
| spellingShingle | J. Allen B. Balasubramanian K. Rankin T. Shah A.M. Donoghue I. Upadhyaya B. Sartini Y. Luo A. Upadhyay Trans-cinnamaldehyde nanoemulsion wash inactivates Salmonella Enteritidis on shelled eggs without affecting egg color Poultry Science trans-cinnamaldehyde salmonella enteritidis nanoemulsion food-grade emulsifier egg |
| title | Trans-cinnamaldehyde nanoemulsion wash inactivates Salmonella Enteritidis on shelled eggs without affecting egg color |
| title_full | Trans-cinnamaldehyde nanoemulsion wash inactivates Salmonella Enteritidis on shelled eggs without affecting egg color |
| title_fullStr | Trans-cinnamaldehyde nanoemulsion wash inactivates Salmonella Enteritidis on shelled eggs without affecting egg color |
| title_full_unstemmed | Trans-cinnamaldehyde nanoemulsion wash inactivates Salmonella Enteritidis on shelled eggs without affecting egg color |
| title_short | Trans-cinnamaldehyde nanoemulsion wash inactivates Salmonella Enteritidis on shelled eggs without affecting egg color |
| title_sort | trans cinnamaldehyde nanoemulsion wash inactivates salmonella enteritidis on shelled eggs without affecting egg color |
| topic | trans-cinnamaldehyde salmonella enteritidis nanoemulsion food-grade emulsifier egg |
| url | http://www.sciencedirect.com/science/article/pii/S0032579123000470 |
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