Unveiling the Secretome of the Fungal Plant Pathogen <i>Neofusicoccum parvum</i> Induced by <i>In Vitro</i> Host Mimicry
<i>Neofusicoccum parvum</i> is a fungal plant pathogen of a wide range of hosts but knowledge about the virulence factors of <i>N. parvum</i> and host–pathogen interactions is rather limited. The molecules involved in the interaction between <i>N. parvum</i> and &...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-09-01
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| Series: | Journal of Fungi |
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| Online Access: | https://www.mdpi.com/2309-608X/8/9/971 |
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| author | Forough Nazar Pour Bruna Pedrosa Micaela Oliveira Cátia Fidalgo Bart Devreese Gonzalez Van Driessche Carina Félix Nuno Rosa Artur Alves Ana Sofia Duarte Ana Cristina Esteves |
| author_facet | Forough Nazar Pour Bruna Pedrosa Micaela Oliveira Cátia Fidalgo Bart Devreese Gonzalez Van Driessche Carina Félix Nuno Rosa Artur Alves Ana Sofia Duarte Ana Cristina Esteves |
| author_sort | Forough Nazar Pour |
| collection | DOAJ |
| description | <i>Neofusicoccum parvum</i> is a fungal plant pathogen of a wide range of hosts but knowledge about the virulence factors of <i>N. parvum</i> and host–pathogen interactions is rather limited. The molecules involved in the interaction between <i>N. parvum</i> and <i>Eucalyptus</i> are mostly unknown, so we used a multi-omics approach to understand pathogen–host interactions. We present the first comprehensive characterization of the <i>in vitro</i> secretome of <i>N. parvum</i> and a prediction of protein–protein interactions using a dry-lab non-targeted interactomics strategy. We used LC-MS to identify <i>N. parvum</i> protein profiles, resulting in the identification of over 400 proteins, from which 117 had a different abundance in the presence of the <i>Eucalyptus</i> stem. Most of the more abundant proteins under host mimicry are involved in plant cell wall degradation (targeting pectin and hemicellulose) consistent with pathogen growth on a plant host. Other proteins identified are involved in adhesion to host tissues, penetration, pathogenesis, or reactive oxygen species generation, involving ribonuclease/ribotoxin domains, putative ricin B lectins, and necrosis elicitors. The overexpression of chitosan synthesis proteins during interaction with the <i>Eucalyptus</i> stem reinforces the hypothesis of an infection strategy involving pathogen masking to avoid host defenses. <i>Neofusicoccum parvum</i> has the molecular apparatus to colonize the host but also actively feed on its living cells and induce necrosis suggesting that this species has a hemibiotrophic lifestyle. |
| first_indexed | 2024-03-09T23:30:06Z |
| format | Article |
| id | doaj.art-4e9d62d2e79041d7a3f1db322f7ed759 |
| institution | Directory Open Access Journal |
| issn | 2309-608X |
| language | English |
| last_indexed | 2024-03-09T23:30:06Z |
| publishDate | 2022-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Journal of Fungi |
| spelling | doaj.art-4e9d62d2e79041d7a3f1db322f7ed7592023-11-23T17:10:08ZengMDPI AGJournal of Fungi2309-608X2022-09-018997110.3390/jof8090971Unveiling the Secretome of the Fungal Plant Pathogen <i>Neofusicoccum parvum</i> Induced by <i>In Vitro</i> Host MimicryForough Nazar Pour0Bruna Pedrosa1Micaela Oliveira2Cátia Fidalgo3Bart Devreese4Gonzalez Van Driessche5Carina Félix6Nuno Rosa7Artur Alves8Ana Sofia Duarte9Ana Cristina Esteves10CESAM, Department of Biology, Campus Universitário de Santiago, University of Aveiro, 3810-193 Aveiro, PortugalCESAM, Department of Biology, Campus Universitário de Santiago, University of Aveiro, 3810-193 Aveiro, PortugalCESAM, Department of Biology, Campus Universitário de Santiago, University of Aveiro, 3810-193 Aveiro, PortugalCESAM, Department of Biology, Campus Universitário de Santiago, University of Aveiro, 3810-193 Aveiro, PortugalDepartment of Biochemistry and Microbiology, Laboratory of Microbiology, Ghent University, 9000 Ghent, BelgiumDepartment of Biochemistry and Microbiology, Laboratory of Microbiology, Ghent University, 9000 Ghent, BelgiumCESAM, Department of Biology, Campus Universitário de Santiago, University of Aveiro, 3810-193 Aveiro, PortugalFaculty of Dental Medicine, Center for Interdisciplinary Research in Health (CIIS), Universidade Católica Portuguesa, 3504-505 Viseu, PortugalCESAM, Department of Biology, Campus Universitário de Santiago, University of Aveiro, 3810-193 Aveiro, PortugalFaculty of Dental Medicine, Center for Interdisciplinary Research in Health (CIIS), Universidade Católica Portuguesa, 3504-505 Viseu, PortugalCESAM, Department of Biology, Campus Universitário de Santiago, University of Aveiro, 3810-193 Aveiro, Portugal<i>Neofusicoccum parvum</i> is a fungal plant pathogen of a wide range of hosts but knowledge about the virulence factors of <i>N. parvum</i> and host–pathogen interactions is rather limited. The molecules involved in the interaction between <i>N. parvum</i> and <i>Eucalyptus</i> are mostly unknown, so we used a multi-omics approach to understand pathogen–host interactions. We present the first comprehensive characterization of the <i>in vitro</i> secretome of <i>N. parvum</i> and a prediction of protein–protein interactions using a dry-lab non-targeted interactomics strategy. We used LC-MS to identify <i>N. parvum</i> protein profiles, resulting in the identification of over 400 proteins, from which 117 had a different abundance in the presence of the <i>Eucalyptus</i> stem. Most of the more abundant proteins under host mimicry are involved in plant cell wall degradation (targeting pectin and hemicellulose) consistent with pathogen growth on a plant host. Other proteins identified are involved in adhesion to host tissues, penetration, pathogenesis, or reactive oxygen species generation, involving ribonuclease/ribotoxin domains, putative ricin B lectins, and necrosis elicitors. The overexpression of chitosan synthesis proteins during interaction with the <i>Eucalyptus</i> stem reinforces the hypothesis of an infection strategy involving pathogen masking to avoid host defenses. <i>Neofusicoccum parvum</i> has the molecular apparatus to colonize the host but also actively feed on its living cells and induce necrosis suggesting that this species has a hemibiotrophic lifestyle.https://www.mdpi.com/2309-608X/8/9/971Botryosphaeriaceae<i>Neofusicoccum parvum</i>plant fungal interactionsecretomeLC-MS<i>Eucalyptus globulus</i> |
| spellingShingle | Forough Nazar Pour Bruna Pedrosa Micaela Oliveira Cátia Fidalgo Bart Devreese Gonzalez Van Driessche Carina Félix Nuno Rosa Artur Alves Ana Sofia Duarte Ana Cristina Esteves Unveiling the Secretome of the Fungal Plant Pathogen <i>Neofusicoccum parvum</i> Induced by <i>In Vitro</i> Host Mimicry Journal of Fungi Botryosphaeriaceae <i>Neofusicoccum parvum</i> plant fungal interaction secretome LC-MS <i>Eucalyptus globulus</i> |
| title | Unveiling the Secretome of the Fungal Plant Pathogen <i>Neofusicoccum parvum</i> Induced by <i>In Vitro</i> Host Mimicry |
| title_full | Unveiling the Secretome of the Fungal Plant Pathogen <i>Neofusicoccum parvum</i> Induced by <i>In Vitro</i> Host Mimicry |
| title_fullStr | Unveiling the Secretome of the Fungal Plant Pathogen <i>Neofusicoccum parvum</i> Induced by <i>In Vitro</i> Host Mimicry |
| title_full_unstemmed | Unveiling the Secretome of the Fungal Plant Pathogen <i>Neofusicoccum parvum</i> Induced by <i>In Vitro</i> Host Mimicry |
| title_short | Unveiling the Secretome of the Fungal Plant Pathogen <i>Neofusicoccum parvum</i> Induced by <i>In Vitro</i> Host Mimicry |
| title_sort | unveiling the secretome of the fungal plant pathogen i neofusicoccum parvum i induced by i in vitro i host mimicry |
| topic | Botryosphaeriaceae <i>Neofusicoccum parvum</i> plant fungal interaction secretome LC-MS <i>Eucalyptus globulus</i> |
| url | https://www.mdpi.com/2309-608X/8/9/971 |
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