The value of p16INK4a immunostaining for high-grade squamous intraepithelial lesions in human papillomavirus-negative patients
Abstract Background This study aims to evaluate the value of p16INK4a immunostaining for high-grade squamous intraepithelial lesions in human papillomavirus-negative patients in Beijing, China. Methods In this study, we evaluated the value of p16INK4a immunostaining, as well as cytology and colposco...
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BMC
2022-04-01
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| Series: | BMC Women's Health |
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| Online Access: | https://doi.org/10.1186/s12905-022-01714-0 |
| _version_ | 1818263879586676736 |
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| author | Dai Zhang Jie Song Xiaosong Zhang Hui Bi |
| author_facet | Dai Zhang Jie Song Xiaosong Zhang Hui Bi |
| author_sort | Dai Zhang |
| collection | DOAJ |
| description | Abstract Background This study aims to evaluate the value of p16INK4a immunostaining for high-grade squamous intraepithelial lesions in human papillomavirus-negative patients in Beijing, China. Methods In this study, we evaluated the value of p16INK4a immunostaining, as well as cytology and colposcopy, for predicting high-grade squamous intraepithelial lesions (HSIL) in human papillomavirus (HPV)-negative patients by comparing the methods with the haematoxylin and eosin (H&E) staining pathological diagnosis of HPV-negative patients. Results Of 122 patients negative for the high-risk HPV (hrHPV) subtype, 26 (21.3%) underwent colposcopically directed multiple punch cervical biopsies with H&E pathological diagnoses of HSIL and above (HSIL+), 11 patients (9.0%) had cervical intraepithelial neoplasia (CIN)2, nine patients (7.4%) had CIN3 and six patients (4.9%) had infiltrating carcinomas. Cytology, colposcopy and p16INK4a immunostaining had 52.4%, 38.5% and 92.3% sensitivity, respectively, and 76.2%, 94.8% and 99% specificity, respectively. The positive predictive value of the cytology, colposcopy and p16INK4a immunostaining was 31.4%, 66.7% and 96%, respectively, and the negative predictive value was 88.5%, 85.1% and 97.9%, respectively. Compared with H&E staining, the kappa of the cytology, colposcopy and p16INK4a immunostaining was 0.327, 0.323 and 0.926, respectively. Conclusion Positive p16INK4a immunostaining is very strongly consistent with an H&E diagnosis of CIN2+, and it can be used as an objective detection index for HSIL+ diagnoses of HPV-negative patients with CIN2+. |
| first_indexed | 2024-12-12T19:26:02Z |
| format | Article |
| id | doaj.art-4e9e19c2da864b139785fa057ed97ff3 |
| institution | Directory Open Access Journal |
| issn | 1472-6874 |
| language | English |
| last_indexed | 2024-12-12T19:26:02Z |
| publishDate | 2022-04-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Women's Health |
| spelling | doaj.art-4e9e19c2da864b139785fa057ed97ff32022-12-22T00:14:30ZengBMCBMC Women's Health1472-68742022-04-012211810.1186/s12905-022-01714-0The value of p16INK4a immunostaining for high-grade squamous intraepithelial lesions in human papillomavirus-negative patientsDai Zhang0Jie Song1Xiaosong Zhang2Hui Bi3Department of Obstetrics and Gynecology, Peking University First HospitalDepartment of Obstetrics and Gynecology, Peking University First HospitalDepartment of Obstetrics and Gynecology, Peking University First HospitalDepartment of Obstetrics and Gynecology, Peking University First HospitalAbstract Background This study aims to evaluate the value of p16INK4a immunostaining for high-grade squamous intraepithelial lesions in human papillomavirus-negative patients in Beijing, China. Methods In this study, we evaluated the value of p16INK4a immunostaining, as well as cytology and colposcopy, for predicting high-grade squamous intraepithelial lesions (HSIL) in human papillomavirus (HPV)-negative patients by comparing the methods with the haematoxylin and eosin (H&E) staining pathological diagnosis of HPV-negative patients. Results Of 122 patients negative for the high-risk HPV (hrHPV) subtype, 26 (21.3%) underwent colposcopically directed multiple punch cervical biopsies with H&E pathological diagnoses of HSIL and above (HSIL+), 11 patients (9.0%) had cervical intraepithelial neoplasia (CIN)2, nine patients (7.4%) had CIN3 and six patients (4.9%) had infiltrating carcinomas. Cytology, colposcopy and p16INK4a immunostaining had 52.4%, 38.5% and 92.3% sensitivity, respectively, and 76.2%, 94.8% and 99% specificity, respectively. The positive predictive value of the cytology, colposcopy and p16INK4a immunostaining was 31.4%, 66.7% and 96%, respectively, and the negative predictive value was 88.5%, 85.1% and 97.9%, respectively. Compared with H&E staining, the kappa of the cytology, colposcopy and p16INK4a immunostaining was 0.327, 0.323 and 0.926, respectively. Conclusion Positive p16INK4a immunostaining is very strongly consistent with an H&E diagnosis of CIN2+, and it can be used as an objective detection index for HSIL+ diagnoses of HPV-negative patients with CIN2+.https://doi.org/10.1186/s12905-022-01714-0p16INK4a immunostainingHigh-grade squamous intraepithelial lesionsHuman papillomavirusCervical cancerColposcopically directed cervical biopsy (CDB) |
| spellingShingle | Dai Zhang Jie Song Xiaosong Zhang Hui Bi The value of p16INK4a immunostaining for high-grade squamous intraepithelial lesions in human papillomavirus-negative patients BMC Women's Health p16INK4a immunostaining High-grade squamous intraepithelial lesions Human papillomavirus Cervical cancer Colposcopically directed cervical biopsy (CDB) |
| title | The value of p16INK4a immunostaining for high-grade squamous intraepithelial lesions in human papillomavirus-negative patients |
| title_full | The value of p16INK4a immunostaining for high-grade squamous intraepithelial lesions in human papillomavirus-negative patients |
| title_fullStr | The value of p16INK4a immunostaining for high-grade squamous intraepithelial lesions in human papillomavirus-negative patients |
| title_full_unstemmed | The value of p16INK4a immunostaining for high-grade squamous intraepithelial lesions in human papillomavirus-negative patients |
| title_short | The value of p16INK4a immunostaining for high-grade squamous intraepithelial lesions in human papillomavirus-negative patients |
| title_sort | value of p16ink4a immunostaining for high grade squamous intraepithelial lesions in human papillomavirus negative patients |
| topic | p16INK4a immunostaining High-grade squamous intraepithelial lesions Human papillomavirus Cervical cancer Colposcopically directed cervical biopsy (CDB) |
| url | https://doi.org/10.1186/s12905-022-01714-0 |
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