Identification of Main-Effect and Environmental Interaction QTL and Their Candidate Genes for Drought Tolerance in a Wheat RIL Population Between Two Elite Spring Cultivars
Understanding the genetics of drought tolerance can expedite the development of drought-tolerant cultivars in wheat. In this study, we dissected the genetics of drought tolerance in spring wheat using a recombinant inbred line (RIL) population derived from a cross between a drought-tolerant cultivar...
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Frontiers Media S.A.
2021-06-01
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| Series: | Frontiers in Genetics |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fgene.2021.656037/full |
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| author | S. M. Hisam Al Rabbi Ajay Kumar Sepehr Mohajeri Naraghi Suraj Sapkota Mohammed S. Alamri Elias M. Elias Shahryar Kianian Raed Seetan Ali Missaoui Ali Missaoui Shyam Solanki Mohamed Mergoum Mohamed Mergoum |
| author_facet | S. M. Hisam Al Rabbi Ajay Kumar Sepehr Mohajeri Naraghi Suraj Sapkota Mohammed S. Alamri Elias M. Elias Shahryar Kianian Raed Seetan Ali Missaoui Ali Missaoui Shyam Solanki Mohamed Mergoum Mohamed Mergoum |
| author_sort | S. M. Hisam Al Rabbi |
| collection | DOAJ |
| description | Understanding the genetics of drought tolerance can expedite the development of drought-tolerant cultivars in wheat. In this study, we dissected the genetics of drought tolerance in spring wheat using a recombinant inbred line (RIL) population derived from a cross between a drought-tolerant cultivar, ‘Reeder’ (PI613586), and a high-yielding but drought-susceptible cultivar, ‘Albany.’ The RIL population was evaluated for grain yield (YLD), grain volume weight (GVW), thousand kernel weight (TKW), plant height (PH), and days to heading (DH) at nine different environments. The Infinium 90 k-based high-density genetic map was generated using 10,657 polymorphic SNP markers representing 2,057 unique loci. Quantitative trait loci (QTL) analysis detected a total of 11 consistent QTL for drought tolerance-related traits. Of these, six QTL were exclusively identified in drought-prone environments, and five were constitutive QTL (identified under both drought and normal conditions). One major QTL on chromosome 7B was identified exclusively under drought environments and explained 13.6% of the phenotypic variation (PV) for YLD. Two other major QTL were detected, one each on chromosomes 7B and 2B under drought-prone environments, and explained 14.86 and 13.94% of phenotypic variation for GVW and YLD, respectively. One novel QTL for drought tolerance was identified on chromosome 2D. In silico expression analysis of candidate genes underlaying the exclusive QTLs associated with drought stress identified the enrichment of ribosomal and chloroplast photosynthesis-associated proteins showing the most expression variability, thus possibly contributing to stress response by modulating the glycosyltransferase (TraesCS6A01G116400) and hexosyltransferase (TraesCS7B01G013300) unique genes present in QTL 21 and 24, respectively. While both parents contributed favorable alleles to these QTL, unexpectedly, the high-yielding and less drought-tolerant parent contributed desirable alleles for drought tolerance at four out of six loci. Regardless of the origin, all QTL with significant drought tolerance could assist significantly in the development of drought-tolerant wheat cultivars, using genomics-assisted breeding approaches. |
| first_indexed | 2024-12-17T00:45:00Z |
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| institution | Directory Open Access Journal |
| issn | 1664-8021 |
| language | English |
| last_indexed | 2024-12-17T00:45:00Z |
| publishDate | 2021-06-01 |
| publisher | Frontiers Media S.A. |
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| series | Frontiers in Genetics |
| spelling | doaj.art-4ea00e0527784919be66f130a789b2b72022-12-21T22:09:55ZengFrontiers Media S.A.Frontiers in Genetics1664-80212021-06-011210.3389/fgene.2021.656037656037Identification of Main-Effect and Environmental Interaction QTL and Their Candidate Genes for Drought Tolerance in a Wheat RIL Population Between Two Elite Spring CultivarsS. M. Hisam Al Rabbi0Ajay Kumar1Sepehr Mohajeri Naraghi2Suraj Sapkota3Mohammed S. Alamri4Elias M. Elias5Shahryar Kianian6Raed Seetan7Ali Missaoui8Ali Missaoui9Shyam Solanki10Mohamed Mergoum11Mohamed Mergoum12Department of Plant Sciences, North Dakota State University, Fargo, ND, United StatesDepartment of Plant Sciences, North Dakota State University, Fargo, ND, United StatesDepartment of Plant Sciences, North Dakota State University, Fargo, ND, United StatesInstitute of Plant Breeding, Genetics, and Genomics, University of Georgia, Griffin, GA, United StatesDepartment of Food Science and Nutrition, King Saud University, Riyadh, Saudi ArabiaDepartment of Plant Sciences, North Dakota State University, Fargo, ND, United StatesUSDA-ARS Cereal Disease Laboratory, University of Minnesota, St. Paul, MN, United StatesDepartment of Computer Science, Slippery Rock University, Slippery Rock, PA, United StatesInstitute of Plant Breeding, Genetics, and Genomics, University of Georgia, Griffin, GA, United StatesDepartment of Crop and Soil Sciences, University of Georgia, Griffin, GA, United StatesDepartment of Crop and Soil Sciences, Washington State University, Pullman, WA, United StatesInstitute of Plant Breeding, Genetics, and Genomics, University of Georgia, Griffin, GA, United StatesDepartment of Crop and Soil Sciences, University of Georgia, Griffin, GA, United StatesUnderstanding the genetics of drought tolerance can expedite the development of drought-tolerant cultivars in wheat. In this study, we dissected the genetics of drought tolerance in spring wheat using a recombinant inbred line (RIL) population derived from a cross between a drought-tolerant cultivar, ‘Reeder’ (PI613586), and a high-yielding but drought-susceptible cultivar, ‘Albany.’ The RIL population was evaluated for grain yield (YLD), grain volume weight (GVW), thousand kernel weight (TKW), plant height (PH), and days to heading (DH) at nine different environments. The Infinium 90 k-based high-density genetic map was generated using 10,657 polymorphic SNP markers representing 2,057 unique loci. Quantitative trait loci (QTL) analysis detected a total of 11 consistent QTL for drought tolerance-related traits. Of these, six QTL were exclusively identified in drought-prone environments, and five were constitutive QTL (identified under both drought and normal conditions). One major QTL on chromosome 7B was identified exclusively under drought environments and explained 13.6% of the phenotypic variation (PV) for YLD. Two other major QTL were detected, one each on chromosomes 7B and 2B under drought-prone environments, and explained 14.86 and 13.94% of phenotypic variation for GVW and YLD, respectively. One novel QTL for drought tolerance was identified on chromosome 2D. In silico expression analysis of candidate genes underlaying the exclusive QTLs associated with drought stress identified the enrichment of ribosomal and chloroplast photosynthesis-associated proteins showing the most expression variability, thus possibly contributing to stress response by modulating the glycosyltransferase (TraesCS6A01G116400) and hexosyltransferase (TraesCS7B01G013300) unique genes present in QTL 21 and 24, respectively. While both parents contributed favorable alleles to these QTL, unexpectedly, the high-yielding and less drought-tolerant parent contributed desirable alleles for drought tolerance at four out of six loci. Regardless of the origin, all QTL with significant drought tolerance could assist significantly in the development of drought-tolerant wheat cultivars, using genomics-assisted breeding approaches.https://www.frontiersin.org/articles/10.3389/fgene.2021.656037/fulldrought tolerancehard red spring wheatquantitative trait locirecombinant inbred linemarker-assisted selection |
| spellingShingle | S. M. Hisam Al Rabbi Ajay Kumar Sepehr Mohajeri Naraghi Suraj Sapkota Mohammed S. Alamri Elias M. Elias Shahryar Kianian Raed Seetan Ali Missaoui Ali Missaoui Shyam Solanki Mohamed Mergoum Mohamed Mergoum Identification of Main-Effect and Environmental Interaction QTL and Their Candidate Genes for Drought Tolerance in a Wheat RIL Population Between Two Elite Spring Cultivars Frontiers in Genetics drought tolerance hard red spring wheat quantitative trait loci recombinant inbred line marker-assisted selection |
| title | Identification of Main-Effect and Environmental Interaction QTL and Their Candidate Genes for Drought Tolerance in a Wheat RIL Population Between Two Elite Spring Cultivars |
| title_full | Identification of Main-Effect and Environmental Interaction QTL and Their Candidate Genes for Drought Tolerance in a Wheat RIL Population Between Two Elite Spring Cultivars |
| title_fullStr | Identification of Main-Effect and Environmental Interaction QTL and Their Candidate Genes for Drought Tolerance in a Wheat RIL Population Between Two Elite Spring Cultivars |
| title_full_unstemmed | Identification of Main-Effect and Environmental Interaction QTL and Their Candidate Genes for Drought Tolerance in a Wheat RIL Population Between Two Elite Spring Cultivars |
| title_short | Identification of Main-Effect and Environmental Interaction QTL and Their Candidate Genes for Drought Tolerance in a Wheat RIL Population Between Two Elite Spring Cultivars |
| title_sort | identification of main effect and environmental interaction qtl and their candidate genes for drought tolerance in a wheat ril population between two elite spring cultivars |
| topic | drought tolerance hard red spring wheat quantitative trait loci recombinant inbred line marker-assisted selection |
| url | https://www.frontiersin.org/articles/10.3389/fgene.2021.656037/full |
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