Regeneration of Escherichia coli Giant Protoplasts to Their Original Form
The spheroplasts and protoplasts of cell wall-deficient (CWD) bacteria are able to revert to their original cellular morphologies through the regeneration of their cell walls. However, whether this is true for giant protoplasts (GPs), which can be as large as 10 μm in diameter, is unknown. GP...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2019-03-01
|
| Series: | Life |
| Subjects: | |
| Online Access: | http://www.mdpi.com/2075-1729/9/1/24 |
| _version_ | 1811187326172266496 |
|---|---|
| author | Kazuhito V. Tabata Takao Sogo Yoshiki Moriizumi Hiroyuki Noji |
| author_facet | Kazuhito V. Tabata Takao Sogo Yoshiki Moriizumi Hiroyuki Noji |
| author_sort | Kazuhito V. Tabata |
| collection | DOAJ |
| description | The spheroplasts and protoplasts of cell wall-deficient (CWD) bacteria are able to revert to their original cellular morphologies through the regeneration of their cell walls. However, whether this is true for giant protoplasts (GPs), which can be as large as 10 μm in diameter, is unknown. GPs can be prepared from various bacteria, including Escherichia coli and Bacillus subtilis, and also from fungi, through culture in the presence of inhibitors for cell wall synthesis or mitosis. In this report, we prepared GPs from E. coli and showed that they can return to rod-shaped bacterium, and that they are capable of colony formation. Microscopic investigation revealed that the regeneration process took place through a variety of morphological pathways. We also report the relationship between GP division and GP volume. Finally, we show that FtsZ is crucial for GP division. These results indicate that E. coli is a highly robust organism that can regenerate its original form from an irregular state, such as GP. |
| first_indexed | 2024-04-11T14:00:19Z |
| format | Article |
| id | doaj.art-4ea39623d7c94cd7ba25ef29ee708c55 |
| institution | Directory Open Access Journal |
| issn | 2075-1729 |
| language | English |
| last_indexed | 2024-04-11T14:00:19Z |
| publishDate | 2019-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Life |
| spelling | doaj.art-4ea39623d7c94cd7ba25ef29ee708c552022-12-22T04:20:08ZengMDPI AGLife2075-17292019-03-01912410.3390/life9010024life9010024Regeneration of Escherichia coli Giant Protoplasts to Their Original FormKazuhito V. Tabata0Takao Sogo1Yoshiki Moriizumi2Hiroyuki Noji3Department of Applied Chemistry, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, JapanDepartment of Applied Chemistry, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, JapanDepartment of Applied Chemistry, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, JapanDepartment of Applied Chemistry, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, JapanThe spheroplasts and protoplasts of cell wall-deficient (CWD) bacteria are able to revert to their original cellular morphologies through the regeneration of their cell walls. However, whether this is true for giant protoplasts (GPs), which can be as large as 10 μm in diameter, is unknown. GPs can be prepared from various bacteria, including Escherichia coli and Bacillus subtilis, and also from fungi, through culture in the presence of inhibitors for cell wall synthesis or mitosis. In this report, we prepared GPs from E. coli and showed that they can return to rod-shaped bacterium, and that they are capable of colony formation. Microscopic investigation revealed that the regeneration process took place through a variety of morphological pathways. We also report the relationship between GP division and GP volume. Finally, we show that FtsZ is crucial for GP division. These results indicate that E. coli is a highly robust organism that can regenerate its original form from an irregular state, such as GP.http://www.mdpi.com/2075-1729/9/1/24giant protoplastEscherichia coliproliferationcell wall-deficient bacteria |
| spellingShingle | Kazuhito V. Tabata Takao Sogo Yoshiki Moriizumi Hiroyuki Noji Regeneration of Escherichia coli Giant Protoplasts to Their Original Form Life giant protoplast Escherichia coli proliferation cell wall-deficient bacteria |
| title | Regeneration of Escherichia coli Giant Protoplasts to Their Original Form |
| title_full | Regeneration of Escherichia coli Giant Protoplasts to Their Original Form |
| title_fullStr | Regeneration of Escherichia coli Giant Protoplasts to Their Original Form |
| title_full_unstemmed | Regeneration of Escherichia coli Giant Protoplasts to Their Original Form |
| title_short | Regeneration of Escherichia coli Giant Protoplasts to Their Original Form |
| title_sort | regeneration of escherichia coli giant protoplasts to their original form |
| topic | giant protoplast Escherichia coli proliferation cell wall-deficient bacteria |
| url | http://www.mdpi.com/2075-1729/9/1/24 |
| work_keys_str_mv | AT kazuhitovtabata regenerationofescherichiacoligiantprotoplaststotheiroriginalform AT takaosogo regenerationofescherichiacoligiantprotoplaststotheiroriginalform AT yoshikimoriizumi regenerationofescherichiacoligiantprotoplaststotheiroriginalform AT hiroyukinoji regenerationofescherichiacoligiantprotoplaststotheiroriginalform |