Novel Biological and Molecular Characterization in Radiopharmaceutical Preclinical Design
In this study, the potential of a digital autoradiography system equipped with a super resolution screen has been evaluated to investigate the biodistribution of a 18F-PSMA inhibitor in a prostate cancer mouse model. Twelve double xenograft NOD/SCID mice (LNCAP and PC3 tumours) were divided into thr...
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-10-01
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| Series: | Journal of Clinical Medicine |
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| Online Access: | https://www.mdpi.com/2077-0383/10/21/4850 |
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| author | Nicoletta Urbano Manuel Scimeca Anna Tolomeo Vincenzo Dimiccoli Elena Bonanno Orazio Schillaci |
| author_facet | Nicoletta Urbano Manuel Scimeca Anna Tolomeo Vincenzo Dimiccoli Elena Bonanno Orazio Schillaci |
| author_sort | Nicoletta Urbano |
| collection | DOAJ |
| description | In this study, the potential of a digital autoradiography system equipped with a super resolution screen has been evaluated to investigate the biodistribution of a 18F-PSMA inhibitor in a prostate cancer mouse model. Twelve double xenograft NOD/SCID mice (LNCAP and PC3 tumours) were divided into three groups according to post-injection time points of an 18F-PSMA inhibitor. Groups of 4 mice were used to evaluate the biodistribution of the radiopharmaceutical after 30-, 60- and 120-min post-injection. Data here reported demonstrated that the digital autoradiography system is suitable to analyse the biodistribution of an 18F-PSMA inhibitor in both whole small-animal bodies and in single organs. The exposure of both whole mouse bodies and organs on the super resolution screen surface allowed the radioactivity of the PSMA inhibitor distributed in the tissues to be detected and quantified. Data obtained by using a digital autoradiography system were in line with the values detected by the activity calibrator. In addition, the image obtained from the super resolution screen allowed a perfect overlap with the tumour images achieved under the optical microscope. In conclusion, biodistribution studies performed by the autoradiography system allow the microscopical modifications induced by therapeutic radiopharmaceuticals to be studied by comparing the molecular imaging and histopathological data at the sub-cellular level. |
| first_indexed | 2024-03-10T05:59:38Z |
| format | Article |
| id | doaj.art-4ea74e1fb08b448ca4a70a8a0ddcf136 |
| institution | Directory Open Access Journal |
| issn | 2077-0383 |
| language | English |
| last_indexed | 2024-03-10T05:59:38Z |
| publishDate | 2021-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Journal of Clinical Medicine |
| spelling | doaj.art-4ea74e1fb08b448ca4a70a8a0ddcf1362023-11-22T21:03:32ZengMDPI AGJournal of Clinical Medicine2077-03832021-10-011021485010.3390/jcm10214850Novel Biological and Molecular Characterization in Radiopharmaceutical Preclinical DesignNicoletta Urbano0Manuel Scimeca1Anna Tolomeo2Vincenzo Dimiccoli3Elena Bonanno4Orazio Schillaci5Nuclear Medicine Unit, Department of Oncohaematology, Policlinico “Tor Vergata”, 00133 Rome, ItalyDepartment of Experimental Medicine, University of Rome “Tor Vergata”, 00133 Rome, ItalyDepartment of ITELPHARMA, ITEL Telecomunicazioni S.R.L., Via Labriola snc, 70037 Ruvo di Puglia, ItalyDepartment of ITELPHARMA, ITEL Telecomunicazioni S.R.L., Via Labriola snc, 70037 Ruvo di Puglia, ItalyDepartment of Experimental Medicine, University of Rome “Tor Vergata”, 00133 Rome, ItalyDepartment of Biomedicine and Prevention, University of Rome “Tor Vergata”, 00133 Rome, ItalyIn this study, the potential of a digital autoradiography system equipped with a super resolution screen has been evaluated to investigate the biodistribution of a 18F-PSMA inhibitor in a prostate cancer mouse model. Twelve double xenograft NOD/SCID mice (LNCAP and PC3 tumours) were divided into three groups according to post-injection time points of an 18F-PSMA inhibitor. Groups of 4 mice were used to evaluate the biodistribution of the radiopharmaceutical after 30-, 60- and 120-min post-injection. Data here reported demonstrated that the digital autoradiography system is suitable to analyse the biodistribution of an 18F-PSMA inhibitor in both whole small-animal bodies and in single organs. The exposure of both whole mouse bodies and organs on the super resolution screen surface allowed the radioactivity of the PSMA inhibitor distributed in the tissues to be detected and quantified. Data obtained by using a digital autoradiography system were in line with the values detected by the activity calibrator. In addition, the image obtained from the super resolution screen allowed a perfect overlap with the tumour images achieved under the optical microscope. In conclusion, biodistribution studies performed by the autoradiography system allow the microscopical modifications induced by therapeutic radiopharmaceuticals to be studied by comparing the molecular imaging and histopathological data at the sub-cellular level.https://www.mdpi.com/2077-0383/10/21/4850nuclear imagingpre-clinical modelradiopharmaceuticaldigital autoradiography system |
| spellingShingle | Nicoletta Urbano Manuel Scimeca Anna Tolomeo Vincenzo Dimiccoli Elena Bonanno Orazio Schillaci Novel Biological and Molecular Characterization in Radiopharmaceutical Preclinical Design Journal of Clinical Medicine nuclear imaging pre-clinical model radiopharmaceutical digital autoradiography system |
| title | Novel Biological and Molecular Characterization in Radiopharmaceutical Preclinical Design |
| title_full | Novel Biological and Molecular Characterization in Radiopharmaceutical Preclinical Design |
| title_fullStr | Novel Biological and Molecular Characterization in Radiopharmaceutical Preclinical Design |
| title_full_unstemmed | Novel Biological and Molecular Characterization in Radiopharmaceutical Preclinical Design |
| title_short | Novel Biological and Molecular Characterization in Radiopharmaceutical Preclinical Design |
| title_sort | novel biological and molecular characterization in radiopharmaceutical preclinical design |
| topic | nuclear imaging pre-clinical model radiopharmaceutical digital autoradiography system |
| url | https://www.mdpi.com/2077-0383/10/21/4850 |
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