Associations of genetic variants contributing to gut microbiota composition in diabetic nephropathy
IntroductionThe gut microbiota is strongly associated with multiple kidney diseases, and since microbial composition is heritable, we hypothesized that genetic variations controlling gut microbiota composition were associated with diabetic nephropathy susceptibility or clinical subphenotypes.Methods...
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| Format: | Article |
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Frontiers Media S.A.
2023-12-01
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| Series: | Frontiers in Endocrinology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fendo.2023.1264517/full |
| _version_ | 1797384882235637760 |
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| author | Xiao Lu Junjun Ma Lili Guo Wei Wu Rongshan Li |
| author_facet | Xiao Lu Junjun Ma Lili Guo Wei Wu Rongshan Li |
| author_sort | Xiao Lu |
| collection | DOAJ |
| description | IntroductionThe gut microbiota is strongly associated with multiple kidney diseases, and since microbial composition is heritable, we hypothesized that genetic variations controlling gut microbiota composition were associated with diabetic nephropathy susceptibility or clinical subphenotypes.MethodsThe genetic variations associated with gut microbiota were retrieved from the genome-wide association study database and analysed in our diabetic nephropathy susceptibility gene screening cohort. Candidate microorganisms with possible genetic associations were identified using the annotation of microbial quantitative trait loci. Finally, the candidate microorganisms were verified by 16S rDNA gene sequencing. ResultsThere were 13 genetic variation loci associated with susceptibility to diabetic nephropathy. The TCF7L2 risk genotype was associated with a long duration of diabetes and high diastolic blood pressure, the ZCWPW2 risk genotype was associated with increased glycosylated hemoglobin, and the ZNRF3 risk genotype was associated with an increased urinary microalbumin-to-creatinine ratio. Both the ZNRF3 and SPECC1L risk genotypes were associated with the abundance of Lactococcus. 16S rDNA sequencing confirmed that there was indeed a significant difference in the Lactococcus genus between DN and DM patients. ConclusionsIn this study, we preliminarily confirmed that the gut microbiota of diabetic nephropathy patients is influenced by host genetics and provide a new basis for future accurate diagnosis and treatment. |
| first_indexed | 2024-03-08T21:45:57Z |
| format | Article |
| id | doaj.art-4ea920da74074ebeb1b06a9fff4773a0 |
| institution | Directory Open Access Journal |
| issn | 1664-2392 |
| language | English |
| last_indexed | 2024-03-08T21:45:57Z |
| publishDate | 2023-12-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Endocrinology |
| spelling | doaj.art-4ea920da74074ebeb1b06a9fff4773a02023-12-20T09:10:33ZengFrontiers Media S.A.Frontiers in Endocrinology1664-23922023-12-011410.3389/fendo.2023.12645171264517Associations of genetic variants contributing to gut microbiota composition in diabetic nephropathyXiao Lu0Junjun Ma1Lili Guo2Wei Wu3Rongshan Li4Department of Nephrology, Fifth Hospital of Shanxi Medical University (Shanxi Provincial People’s Hospital), Taiyuan, ChinaDepartment of Thoracic Surgery, Fifth Hospital of Shanxi Medical University (Shanxi Provincial People’s Hospital), Taiyuan, ChinaShanxi Provincial Key Laboratory of Kidney Disease, Fifth Hospital of Shanxi Medical University (Shanxi Provincial People’s Hospital), Taiyuan, ChinaDepartment of Clinical Laboratory, Fifth Hospital of Shanxi Medical University (Shanxi Provincial People’s Hospital), Taiyuan, ChinaDepartment of Clinical Laboratory, Fifth Hospital of Shanxi Medical University (Shanxi Provincial People’s Hospital), Taiyuan, ChinaIntroductionThe gut microbiota is strongly associated with multiple kidney diseases, and since microbial composition is heritable, we hypothesized that genetic variations controlling gut microbiota composition were associated with diabetic nephropathy susceptibility or clinical subphenotypes.MethodsThe genetic variations associated with gut microbiota were retrieved from the genome-wide association study database and analysed in our diabetic nephropathy susceptibility gene screening cohort. Candidate microorganisms with possible genetic associations were identified using the annotation of microbial quantitative trait loci. Finally, the candidate microorganisms were verified by 16S rDNA gene sequencing. ResultsThere were 13 genetic variation loci associated with susceptibility to diabetic nephropathy. The TCF7L2 risk genotype was associated with a long duration of diabetes and high diastolic blood pressure, the ZCWPW2 risk genotype was associated with increased glycosylated hemoglobin, and the ZNRF3 risk genotype was associated with an increased urinary microalbumin-to-creatinine ratio. Both the ZNRF3 and SPECC1L risk genotypes were associated with the abundance of Lactococcus. 16S rDNA sequencing confirmed that there was indeed a significant difference in the Lactococcus genus between DN and DM patients. ConclusionsIn this study, we preliminarily confirmed that the gut microbiota of diabetic nephropathy patients is influenced by host genetics and provide a new basis for future accurate diagnosis and treatment.https://www.frontiersin.org/articles/10.3389/fendo.2023.1264517/fulldiabetic nephropathydiabetes mellitussusceptibility genesgut microbiotamicrobial quantitative trait locus |
| spellingShingle | Xiao Lu Junjun Ma Lili Guo Wei Wu Rongshan Li Associations of genetic variants contributing to gut microbiota composition in diabetic nephropathy Frontiers in Endocrinology diabetic nephropathy diabetes mellitus susceptibility genes gut microbiota microbial quantitative trait locus |
| title | Associations of genetic variants contributing to gut microbiota composition in diabetic nephropathy |
| title_full | Associations of genetic variants contributing to gut microbiota composition in diabetic nephropathy |
| title_fullStr | Associations of genetic variants contributing to gut microbiota composition in diabetic nephropathy |
| title_full_unstemmed | Associations of genetic variants contributing to gut microbiota composition in diabetic nephropathy |
| title_short | Associations of genetic variants contributing to gut microbiota composition in diabetic nephropathy |
| title_sort | associations of genetic variants contributing to gut microbiota composition in diabetic nephropathy |
| topic | diabetic nephropathy diabetes mellitus susceptibility genes gut microbiota microbial quantitative trait locus |
| url | https://www.frontiersin.org/articles/10.3389/fendo.2023.1264517/full |
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