Comparative evaluation of inhibitory effect of curcumin and doxycycline on matrix metalloproteinase-9 activity in chronic periodontitis
Background and Objectives: The pathogenesis of inflammatory periodontal diseases essentially involves degradation of extracellular matrix molecules, and collagen breakdown and matrix metalloproteinases (MMPs) are proteinases primarily involved in this process. It is known that doxycycline downregula...
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| Format: | Article |
| Language: | English |
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Wolters Kluwer Medknow Publications
2017-01-01
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| Series: | Indian Journal of Dental Research |
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| Online Access: | http://www.ijdr.in/article.asp?issn=0970-9290;year=2017;volume=28;issue=5;spage=560;epage=565;aulast=Guru |
| _version_ | 1818997638050086912 |
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| author | Sanjeela Rakshith Guru Shaila V Kothiwale Nitin Saroch Rakshith Chander Guru |
| author_facet | Sanjeela Rakshith Guru Shaila V Kothiwale Nitin Saroch Rakshith Chander Guru |
| author_sort | Sanjeela Rakshith Guru |
| collection | DOAJ |
| description | Background and Objectives: The pathogenesis of inflammatory periodontal diseases essentially involves degradation of extracellular matrix molecules, and collagen breakdown and matrix metalloproteinases (MMPs) are proteinases primarily involved in this process. It is known that doxycycline downregulates MMP activity. Curcumin has anti-inflammatory effect and also downregulates MMP activity. Thus, a study was conducted to evaluate the anti-inflammatory effect of curcumin by its inhibition of MMP-9 activity and compare the same with doxcycline, which has known anticollagenase activity. Subjects and Methods: Gingival tissue samples were obtained from thirty patients diagnosed with chronic periodontitis. The tissue extracts were treated with Curcumin and doxycycline and inhibition of MMP-9 analyzed by gelatinzymography. Gels obtained were stained with Coomassie Brilliant Blue, and enzymatic activities detected as bands of gelatinlysis against blue background. Relative MMP-9 levels were measured by scanning the clear zones and analyzing the percentage inhibition. Results: Results showed that MMP-9 activity was significantly decreased by both the drugs. Curcumin showed 61.01% reduction in the MMP-9 activity at 1500 μg/ml concentration and doxycycline showed 59.58% reduction in the MMP-9 activity at 300 μg/ml concentration. Conclusion: The current study showed that curcumin has inhibitory effect on polymorphonuclear leukocyte-type MMP-9 involved in matrix degradation in periodontitis. Since Curcumin has a potent anti-inflammatory effect, it may have therapeutic potential as a host modulation agent in periodontal diseases. |
| first_indexed | 2024-12-20T21:48:48Z |
| format | Article |
| id | doaj.art-4eaa2189228d44df8c51449063165cbe |
| institution | Directory Open Access Journal |
| issn | 0970-9290 1998-3603 |
| language | English |
| last_indexed | 2024-12-20T21:48:48Z |
| publishDate | 2017-01-01 |
| publisher | Wolters Kluwer Medknow Publications |
| record_format | Article |
| series | Indian Journal of Dental Research |
| spelling | doaj.art-4eaa2189228d44df8c51449063165cbe2022-12-21T19:25:37ZengWolters Kluwer Medknow PublicationsIndian Journal of Dental Research0970-92901998-36032017-01-0128556056510.4103/ijdr.IJDR_461_16Comparative evaluation of inhibitory effect of curcumin and doxycycline on matrix metalloproteinase-9 activity in chronic periodontitisSanjeela Rakshith GuruShaila V KothiwaleNitin SarochRakshith Chander GuruBackground and Objectives: The pathogenesis of inflammatory periodontal diseases essentially involves degradation of extracellular matrix molecules, and collagen breakdown and matrix metalloproteinases (MMPs) are proteinases primarily involved in this process. It is known that doxycycline downregulates MMP activity. Curcumin has anti-inflammatory effect and also downregulates MMP activity. Thus, a study was conducted to evaluate the anti-inflammatory effect of curcumin by its inhibition of MMP-9 activity and compare the same with doxcycline, which has known anticollagenase activity. Subjects and Methods: Gingival tissue samples were obtained from thirty patients diagnosed with chronic periodontitis. The tissue extracts were treated with Curcumin and doxycycline and inhibition of MMP-9 analyzed by gelatinzymography. Gels obtained were stained with Coomassie Brilliant Blue, and enzymatic activities detected as bands of gelatinlysis against blue background. Relative MMP-9 levels were measured by scanning the clear zones and analyzing the percentage inhibition. Results: Results showed that MMP-9 activity was significantly decreased by both the drugs. Curcumin showed 61.01% reduction in the MMP-9 activity at 1500 μg/ml concentration and doxycycline showed 59.58% reduction in the MMP-9 activity at 300 μg/ml concentration. Conclusion: The current study showed that curcumin has inhibitory effect on polymorphonuclear leukocyte-type MMP-9 involved in matrix degradation in periodontitis. Since Curcumin has a potent anti-inflammatory effect, it may have therapeutic potential as a host modulation agent in periodontal diseases.http://www.ijdr.in/article.asp?issn=0970-9290;year=2017;volume=28;issue=5;spage=560;epage=565;aulast=GuruAnti-inflammatory agentshost modulation therapymatrix metalloproteinasesperiodontitis |
| spellingShingle | Sanjeela Rakshith Guru Shaila V Kothiwale Nitin Saroch Rakshith Chander Guru Comparative evaluation of inhibitory effect of curcumin and doxycycline on matrix metalloproteinase-9 activity in chronic periodontitis Indian Journal of Dental Research Anti-inflammatory agents host modulation therapy matrix metalloproteinases periodontitis |
| title | Comparative evaluation of inhibitory effect of curcumin and doxycycline on matrix metalloproteinase-9 activity in chronic periodontitis |
| title_full | Comparative evaluation of inhibitory effect of curcumin and doxycycline on matrix metalloproteinase-9 activity in chronic periodontitis |
| title_fullStr | Comparative evaluation of inhibitory effect of curcumin and doxycycline on matrix metalloproteinase-9 activity in chronic periodontitis |
| title_full_unstemmed | Comparative evaluation of inhibitory effect of curcumin and doxycycline on matrix metalloproteinase-9 activity in chronic periodontitis |
| title_short | Comparative evaluation of inhibitory effect of curcumin and doxycycline on matrix metalloproteinase-9 activity in chronic periodontitis |
| title_sort | comparative evaluation of inhibitory effect of curcumin and doxycycline on matrix metalloproteinase 9 activity in chronic periodontitis |
| topic | Anti-inflammatory agents host modulation therapy matrix metalloproteinases periodontitis |
| url | http://www.ijdr.in/article.asp?issn=0970-9290;year=2017;volume=28;issue=5;spage=560;epage=565;aulast=Guru |
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