Comparison of the gut microbiota composition between wild and captive sika deer (Cervus nippon hortulorum) from feces by high-throughput sequencing
Abstract The gut microbiota is characterized as a complex ecosystem that has effects on health and diseases of host with the interactions of many other factors together. Sika deer is the national level for the protection of wild animals in China. The available sequencing data of gut microbiota from...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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SpringerOpen
2017-11-01
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| Series: | AMB Express |
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| Online Access: | http://link.springer.com/article/10.1186/s13568-017-0517-8 |
| _version_ | 1818339271937884160 |
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| author | Yu Guan Haitao Yang Siyu Han Limin Feng Tianming Wang Jianping Ge |
| author_facet | Yu Guan Haitao Yang Siyu Han Limin Feng Tianming Wang Jianping Ge |
| author_sort | Yu Guan |
| collection | DOAJ |
| description | Abstract The gut microbiota is characterized as a complex ecosystem that has effects on health and diseases of host with the interactions of many other factors together. Sika deer is the national level for the protection of wild animals in China. The available sequencing data of gut microbiota from feces of wild sika deer, especially for Cervus nippon hortulorum in Northeast China, are limited. Here, we characterized the gastrointestinal bacterial communities of wild (7 samples) and captive (12 samples) sika deer from feces, and compared their gut microbiota by analyzing the V3–V4 region of 16S rRNA gene using high-throughput sequencing technology on the Illumina Hiseq platform. Firmicutes (77.624%), Bacteroidetes (18.288%) and Tenericutes (1.342%) were the most predominant phyla in wild sika deer. While in captive sika deer, Firmicutes (50.710%) was the dominant phylum, followed by Bacteroidetes (31.996%) and Proteobacteria (4.806%). A total of 9 major phyla, 22 families and 30 genera among gastrointestinal bacterial communities showed significant differences between wild and captive sika deer. The specific function and mechanism of Tenericutes in wild sika deer need further study. Our results indicated that captive sika deer in farm had higher fecal bacterial diversity than the wild. Abundance and quantity of diet source for sika deer played crucial role in shaping the composition and structure of gut microbiota. |
| first_indexed | 2024-12-13T15:24:22Z |
| format | Article |
| id | doaj.art-4eabab16935e4501ba7e7d22818fb2d8 |
| institution | Directory Open Access Journal |
| issn | 2191-0855 |
| language | English |
| last_indexed | 2024-12-13T15:24:22Z |
| publishDate | 2017-11-01 |
| publisher | SpringerOpen |
| record_format | Article |
| series | AMB Express |
| spelling | doaj.art-4eabab16935e4501ba7e7d22818fb2d82022-12-21T23:40:24ZengSpringerOpenAMB Express2191-08552017-11-017111310.1186/s13568-017-0517-8Comparison of the gut microbiota composition between wild and captive sika deer (Cervus nippon hortulorum) from feces by high-throughput sequencingYu Guan0Haitao Yang1Siyu Han2Limin Feng3Tianming Wang4Jianping Ge5Ministry of Education Key Laboratory for Biodiversity Science and Engineering and College of Life Sciences, Beijing Normal UniversityMinistry of Education Key Laboratory for Biodiversity Science and Engineering and College of Life Sciences, Beijing Normal UniversityMinistry of Education Key Laboratory for Biodiversity Science and Engineering and College of Life Sciences, Beijing Normal UniversityMinistry of Education Key Laboratory for Biodiversity Science and Engineering and College of Life Sciences, Beijing Normal UniversityMinistry of Education Key Laboratory for Biodiversity Science and Engineering and College of Life Sciences, Beijing Normal UniversityMinistry of Education Key Laboratory for Biodiversity Science and Engineering and College of Life Sciences, Beijing Normal UniversityAbstract The gut microbiota is characterized as a complex ecosystem that has effects on health and diseases of host with the interactions of many other factors together. Sika deer is the national level for the protection of wild animals in China. The available sequencing data of gut microbiota from feces of wild sika deer, especially for Cervus nippon hortulorum in Northeast China, are limited. Here, we characterized the gastrointestinal bacterial communities of wild (7 samples) and captive (12 samples) sika deer from feces, and compared their gut microbiota by analyzing the V3–V4 region of 16S rRNA gene using high-throughput sequencing technology on the Illumina Hiseq platform. Firmicutes (77.624%), Bacteroidetes (18.288%) and Tenericutes (1.342%) were the most predominant phyla in wild sika deer. While in captive sika deer, Firmicutes (50.710%) was the dominant phylum, followed by Bacteroidetes (31.996%) and Proteobacteria (4.806%). A total of 9 major phyla, 22 families and 30 genera among gastrointestinal bacterial communities showed significant differences between wild and captive sika deer. The specific function and mechanism of Tenericutes in wild sika deer need further study. Our results indicated that captive sika deer in farm had higher fecal bacterial diversity than the wild. Abundance and quantity of diet source for sika deer played crucial role in shaping the composition and structure of gut microbiota.http://link.springer.com/article/10.1186/s13568-017-0517-8Sika deer (Cervus nippon hortulorum)Gut microbiota16s rRNA geneHigh-throughput sequencing |
| spellingShingle | Yu Guan Haitao Yang Siyu Han Limin Feng Tianming Wang Jianping Ge Comparison of the gut microbiota composition between wild and captive sika deer (Cervus nippon hortulorum) from feces by high-throughput sequencing AMB Express Sika deer (Cervus nippon hortulorum) Gut microbiota 16s rRNA gene High-throughput sequencing |
| title | Comparison of the gut microbiota composition between wild and captive sika deer (Cervus nippon hortulorum) from feces by high-throughput sequencing |
| title_full | Comparison of the gut microbiota composition between wild and captive sika deer (Cervus nippon hortulorum) from feces by high-throughput sequencing |
| title_fullStr | Comparison of the gut microbiota composition between wild and captive sika deer (Cervus nippon hortulorum) from feces by high-throughput sequencing |
| title_full_unstemmed | Comparison of the gut microbiota composition between wild and captive sika deer (Cervus nippon hortulorum) from feces by high-throughput sequencing |
| title_short | Comparison of the gut microbiota composition between wild and captive sika deer (Cervus nippon hortulorum) from feces by high-throughput sequencing |
| title_sort | comparison of the gut microbiota composition between wild and captive sika deer cervus nippon hortulorum from feces by high throughput sequencing |
| topic | Sika deer (Cervus nippon hortulorum) Gut microbiota 16s rRNA gene High-throughput sequencing |
| url | http://link.springer.com/article/10.1186/s13568-017-0517-8 |
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