Complex Conformational Dynamics of the Heart Failure-Associated Pre-miRNA-377 Hairpin Revealed by Single-Molecule Optical Tweezers

Complex Conformational Dynamics of the Heart Failure-Associated Pre-miRNA-377 Hairpin Revealed by Single-Molecule Optical Tweezers

Pre-miRNA-377 is a hairpin-shaped regulatory RNA associated with heart failure. Here, we use single-molecule optical tweezers to unzip pre-miRNA-377 and study its stability and dynamics. We show that magnesium ions have a strong stabilizing effect, and that sodium ions stabilize the hairpin more tha...

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Bibliographic Details
Main Authors: Anna Wypijewska del Nogal, Vinoth Sundar Rajan, Fredrik Westerlund, L. Marcus Wilhelmsson
Format: Article
Language:English
Published: MDPI AG 2021-08-01
Series:International Journal of Molecular Sciences
Subjects:
single-molecule force spectroscopy
optical tweezers
RNA dynamics
RNA folding heterogeneity
miRNA
pre-miRNA
Online Access:https://www.mdpi.com/1422-0067/22/16/9008

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