Kaempferol and Its Glycoside Derivatives as Modulators of Etoposide Activity in HL-60 Cells
Kaempferol is a polyphenol found in a variety of plants. Kaempferol exerts antitumor properties by affecting proliferation and apoptosis of cancer cells. We investigated whether kaempferol and its glycoside derivatives—kaempferol 3-O-[(6-O-E-caffeoyl)-β-D-glucopyranosyl-(1→2)]-β-D-galactopyranoside-...
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MDPI AG
2021-03-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/22/7/3520 |
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| author | Magdalena Kluska Michał Juszczak Jerzy Żuchowski Anna Stochmal Katarzyna Woźniak |
| author_facet | Magdalena Kluska Michał Juszczak Jerzy Żuchowski Anna Stochmal Katarzyna Woźniak |
| author_sort | Magdalena Kluska |
| collection | DOAJ |
| description | Kaempferol is a polyphenol found in a variety of plants. Kaempferol exerts antitumor properties by affecting proliferation and apoptosis of cancer cells. We investigated whether kaempferol and its glycoside derivatives—kaempferol 3-O-[(6-O-E-caffeoyl)-β-D-glucopyranosyl-(1→2)]-β-D-galactopyranoside-7-O-β-D-glucuropyranoside (P2), kaempferol 3-O-[(6-O-E-p-coumaroyl)-β-D-glucopyranosyl-(1→2)]-β-D-galactopyranoside-7-O-β-D-glucuropyranoside (P5) and kaempferol 3-O-[(6-O-E-feruloyl)-β-D-glucopyranosyl-(1→2)]-β-D-galactopyranoside-7-O-β-D-glucuropyranoside (P7), isolated from aerial parts of <i>Lens culinaris</i> Medik.—affect the antitumor activity of etoposide in human promyelocytic leukemia (HL-60) cells. We analyzed the effect of kaempferol and its derivatives on cytotoxicity, DNA damage, apoptosis, cell cycle progression and free radicals induced by etoposide. We demonstrated that kaempferol increases the sensitivity of HL-60 cells to etoposide but does not affect apoptosis induced by this drug. Kaempferol also reduces the level of free radicals generated by etoposide. Unlike kaempferol, some of its derivatives reduce the apoptosis of HL-60 cells (P2 and P7) and increase the level of free radicals (P2 and P5) induced by etoposide. Our results indicate that kaempferol and its glycoside derivatives can modulate the activity of etoposide in HL-60 cells and affect its antitumor efficacy in this way. Kaempferol derivatives may have the opposite effect on the action of etoposide in HL-60 cells compared to kaempferol. |
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| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-10T12:49:15Z |
| publishDate | 2021-03-01 |
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| series | International Journal of Molecular Sciences |
| spelling | doaj.art-4eac2ac320f240e08413271916d1c7cb2023-11-21T13:14:12ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672021-03-01227352010.3390/ijms22073520Kaempferol and Its Glycoside Derivatives as Modulators of Etoposide Activity in HL-60 CellsMagdalena Kluska0Michał Juszczak1Jerzy Żuchowski2Anna Stochmal3Katarzyna Woźniak4Department of Molecular Genetics, Faculty of Biology and Environmental Protection, University of Lodz, 90-236 Lodz, PolandDepartment of Molecular Genetics, Faculty of Biology and Environmental Protection, University of Lodz, 90-236 Lodz, PolandDepartment of Biochemistry and Crop Quality, Institute of Soil Science and Plant Cultivation, State Research Institute, 24-100 Pulawy, PolandDepartment of Biochemistry and Crop Quality, Institute of Soil Science and Plant Cultivation, State Research Institute, 24-100 Pulawy, PolandDepartment of Molecular Genetics, Faculty of Biology and Environmental Protection, University of Lodz, 90-236 Lodz, PolandKaempferol is a polyphenol found in a variety of plants. Kaempferol exerts antitumor properties by affecting proliferation and apoptosis of cancer cells. We investigated whether kaempferol and its glycoside derivatives—kaempferol 3-O-[(6-O-E-caffeoyl)-β-D-glucopyranosyl-(1→2)]-β-D-galactopyranoside-7-O-β-D-glucuropyranoside (P2), kaempferol 3-O-[(6-O-E-p-coumaroyl)-β-D-glucopyranosyl-(1→2)]-β-D-galactopyranoside-7-O-β-D-glucuropyranoside (P5) and kaempferol 3-O-[(6-O-E-feruloyl)-β-D-glucopyranosyl-(1→2)]-β-D-galactopyranoside-7-O-β-D-glucuropyranoside (P7), isolated from aerial parts of <i>Lens culinaris</i> Medik.—affect the antitumor activity of etoposide in human promyelocytic leukemia (HL-60) cells. We analyzed the effect of kaempferol and its derivatives on cytotoxicity, DNA damage, apoptosis, cell cycle progression and free radicals induced by etoposide. We demonstrated that kaempferol increases the sensitivity of HL-60 cells to etoposide but does not affect apoptosis induced by this drug. Kaempferol also reduces the level of free radicals generated by etoposide. Unlike kaempferol, some of its derivatives reduce the apoptosis of HL-60 cells (P2 and P7) and increase the level of free radicals (P2 and P5) induced by etoposide. Our results indicate that kaempferol and its glycoside derivatives can modulate the activity of etoposide in HL-60 cells and affect its antitumor efficacy in this way. Kaempferol derivatives may have the opposite effect on the action of etoposide in HL-60 cells compared to kaempferol.https://www.mdpi.com/1422-0067/22/7/3520kaempferoletoposideDNA damageapoptosiscell cycleoxidative stress |
| spellingShingle | Magdalena Kluska Michał Juszczak Jerzy Żuchowski Anna Stochmal Katarzyna Woźniak Kaempferol and Its Glycoside Derivatives as Modulators of Etoposide Activity in HL-60 Cells International Journal of Molecular Sciences kaempferol etoposide DNA damage apoptosis cell cycle oxidative stress |
| title | Kaempferol and Its Glycoside Derivatives as Modulators of Etoposide Activity in HL-60 Cells |
| title_full | Kaempferol and Its Glycoside Derivatives as Modulators of Etoposide Activity in HL-60 Cells |
| title_fullStr | Kaempferol and Its Glycoside Derivatives as Modulators of Etoposide Activity in HL-60 Cells |
| title_full_unstemmed | Kaempferol and Its Glycoside Derivatives as Modulators of Etoposide Activity in HL-60 Cells |
| title_short | Kaempferol and Its Glycoside Derivatives as Modulators of Etoposide Activity in HL-60 Cells |
| title_sort | kaempferol and its glycoside derivatives as modulators of etoposide activity in hl 60 cells |
| topic | kaempferol etoposide DNA damage apoptosis cell cycle oxidative stress |
| url | https://www.mdpi.com/1422-0067/22/7/3520 |
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