Green Synthesis and Characterization of Silver Nanoparticles Using <i>Spondias mombin</i> Extract and Their Antimicrobial Activity against Biofilm-Producing Bacteria
Multidrug resistant bacteria create a challenging situation for society to treat infections. Multidrug resistance (MDR) is the reason for biofilm bacteria to cause chronic infection. Plant-based nanoparticles could be an alternative solution as potential drug candidates against these MDR bacteria, a...
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| Format: | Article |
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MDPI AG
2021-05-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/26/9/2681 |
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| author | Sumitha Samuggam Suresh V. Chinni Prasanna Mutusamy Subash C. B. Gopinath Periasamy Anbu Vijayan Venugopal Lebaka Veeranjaneya Reddy Balaji Enugutti |
| author_facet | Sumitha Samuggam Suresh V. Chinni Prasanna Mutusamy Subash C. B. Gopinath Periasamy Anbu Vijayan Venugopal Lebaka Veeranjaneya Reddy Balaji Enugutti |
| author_sort | Sumitha Samuggam |
| collection | DOAJ |
| description | Multidrug resistant bacteria create a challenging situation for society to treat infections. Multidrug resistance (MDR) is the reason for biofilm bacteria to cause chronic infection. Plant-based nanoparticles could be an alternative solution as potential drug candidates against these MDR bacteria, as many plants are well known for their antimicrobial activity against pathogenic microorganisms. <i>Spondias mombin</i> is a traditional plant which has already been used for medicinal purposes as every part of this plant has been proven to have its own medicinal values. In this research, the <i>S. mombin</i> extract was used to synthesise AgNPs. The synthesized AgNPs were characterized and further tested for their antibacterial, reactive oxygen species and cytotoxicity properties. The characterization results showed the synthesized AgNPs to be between 8 to 50 nm with -11.52 of zeta potential value. The existence of the silver element in the AgNPs was confirmed with the peaks obtained in the EDX spectrometry. Significant antibacterial activity was observed against selected biofilm-forming pathogenic bacteria. The cytotoxicity study with <i>A. salina</i> revealed the LC50 of synthesized AgNPs was at 0.81 mg/mL. Based on the ROS quantification, it was suggested that the ROS production, due to the interaction of AgNP with different bacterial cells, causes structural changes of the cell. This proves that the synthesized AgNPs could be an effective drug against multidrug resistant bacteria. |
| first_indexed | 2024-03-10T11:43:17Z |
| format | Article |
| id | doaj.art-4eae3c45cab94ea8a279497ad7a980c7 |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-10T11:43:17Z |
| publishDate | 2021-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-4eae3c45cab94ea8a279497ad7a980c72023-11-21T18:17:18ZengMDPI AGMolecules1420-30492021-05-01269268110.3390/molecules26092681Green Synthesis and Characterization of Silver Nanoparticles Using <i>Spondias mombin</i> Extract and Their Antimicrobial Activity against Biofilm-Producing BacteriaSumitha Samuggam0Suresh V. Chinni1Prasanna Mutusamy2Subash C. B. Gopinath3Periasamy Anbu4Vijayan Venugopal5Lebaka Veeranjaneya Reddy6Balaji Enugutti7Department of Biotechnology, Faculty of Applied Sciences, AIMST University, Bedong 08100, Kedah, MalaysiaDepartment of Biotechnology, Faculty of Applied Sciences, AIMST University, Bedong 08100, Kedah, MalaysiaDepartment of Biotechnology, Faculty of Applied Sciences, AIMST University, Bedong 08100, Kedah, MalaysiaInstitute of Nano Electronic Engineering, Faculty of Chemical Engineering Technology, Universiti Malaysia Perlis, Arau 01000, Perlis, MalaysiaDepartment of Biological Engineering, Inha University, Incheon 402-751, KoreaSchool of Pharmacy, Sri Balaji Vidyapeeth, Deemed to Be University, Puducherry 607402, IndiaDepartment of Microbiology, Yogi Vemana University, Kadapa 516005, IndiaGregor Mendel Institute (GMI), Austrian Academy of Sciences, Vienna Biocenter (VBC), Dr. Bohr-Gasse 3, 1030 Vienna, AustriaMultidrug resistant bacteria create a challenging situation for society to treat infections. Multidrug resistance (MDR) is the reason for biofilm bacteria to cause chronic infection. Plant-based nanoparticles could be an alternative solution as potential drug candidates against these MDR bacteria, as many plants are well known for their antimicrobial activity against pathogenic microorganisms. <i>Spondias mombin</i> is a traditional plant which has already been used for medicinal purposes as every part of this plant has been proven to have its own medicinal values. In this research, the <i>S. mombin</i> extract was used to synthesise AgNPs. The synthesized AgNPs were characterized and further tested for their antibacterial, reactive oxygen species and cytotoxicity properties. The characterization results showed the synthesized AgNPs to be between 8 to 50 nm with -11.52 of zeta potential value. The existence of the silver element in the AgNPs was confirmed with the peaks obtained in the EDX spectrometry. Significant antibacterial activity was observed against selected biofilm-forming pathogenic bacteria. The cytotoxicity study with <i>A. salina</i> revealed the LC50 of synthesized AgNPs was at 0.81 mg/mL. Based on the ROS quantification, it was suggested that the ROS production, due to the interaction of AgNP with different bacterial cells, causes structural changes of the cell. This proves that the synthesized AgNPs could be an effective drug against multidrug resistant bacteria.https://www.mdpi.com/1420-3049/26/9/2681<i>Spondias mombin</i>AgNPbiofilm bacteria |
| spellingShingle | Sumitha Samuggam Suresh V. Chinni Prasanna Mutusamy Subash C. B. Gopinath Periasamy Anbu Vijayan Venugopal Lebaka Veeranjaneya Reddy Balaji Enugutti Green Synthesis and Characterization of Silver Nanoparticles Using <i>Spondias mombin</i> Extract and Their Antimicrobial Activity against Biofilm-Producing Bacteria Molecules <i>Spondias mombin</i> AgNP biofilm bacteria |
| title | Green Synthesis and Characterization of Silver Nanoparticles Using <i>Spondias mombin</i> Extract and Their Antimicrobial Activity against Biofilm-Producing Bacteria |
| title_full | Green Synthesis and Characterization of Silver Nanoparticles Using <i>Spondias mombin</i> Extract and Their Antimicrobial Activity against Biofilm-Producing Bacteria |
| title_fullStr | Green Synthesis and Characterization of Silver Nanoparticles Using <i>Spondias mombin</i> Extract and Their Antimicrobial Activity against Biofilm-Producing Bacteria |
| title_full_unstemmed | Green Synthesis and Characterization of Silver Nanoparticles Using <i>Spondias mombin</i> Extract and Their Antimicrobial Activity against Biofilm-Producing Bacteria |
| title_short | Green Synthesis and Characterization of Silver Nanoparticles Using <i>Spondias mombin</i> Extract and Their Antimicrobial Activity against Biofilm-Producing Bacteria |
| title_sort | green synthesis and characterization of silver nanoparticles using i spondias mombin i extract and their antimicrobial activity against biofilm producing bacteria |
| topic | <i>Spondias mombin</i> AgNP biofilm bacteria |
| url | https://www.mdpi.com/1420-3049/26/9/2681 |
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