Expression Profile and Potential Function of Circular RNAs in Peripheral Blood Mononuclear Cells in Male Patients With Primary Gout
Circular RNAs (circRNAs) are non-coding RNAs (ncRNAs) with a single-stranded covalently closed-loop structure, and their abnormal expression may participate in the pathogenesis of various human diseases. Currently, knowledge of circRNAs in gout is limited. In this case-control study, human circRNA m...
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Frontiers Media S.A.
2021-10-01
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| Series: | Frontiers in Genetics |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fgene.2021.728091/full |
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| author | Fei Dai Fei Dai Quan-Bo Zhang Quan-Bo Zhang Yi-Ping Tang Yi-Ping Tang Yi-Xi He Yi-Xi He Ting Yi Ting Yi Yu-Feng Qing Yu-Feng Qing |
| author_facet | Fei Dai Fei Dai Quan-Bo Zhang Quan-Bo Zhang Yi-Ping Tang Yi-Ping Tang Yi-Xi He Yi-Xi He Ting Yi Ting Yi Yu-Feng Qing Yu-Feng Qing |
| author_sort | Fei Dai |
| collection | DOAJ |
| description | Circular RNAs (circRNAs) are non-coding RNAs (ncRNAs) with a single-stranded covalently closed-loop structure, and their abnormal expression may participate in the pathogenesis of various human diseases. Currently, knowledge of circRNAs in gout is limited. In this case-control study, human circRNA microarrays were used to identify differentially expressed circRNAs in peripheral blood mononuclear cells (PBMCs) from patients with primary gout (n = 5) and healthy controls (HC; n = 3). Bioinformatics methods were used to analyze significantly different circRNAs (fold change >1.5, p < 0.05). In addition, four significantly differentially expressed circRNAs were selected for quantitative real-time polymerase chain reaction to detect expression levels in 90 gout patients and 60 HC. Subsequently, circRNA-miRNA-mRNA network was established to predict the function of circRNAs of interest. Microarray analysis indicated that 238 circRNAs were upregulated and 41 circRNAs were down-regulated in the gout group (fold change >1.5, p < 0.05). Bioinformatics analysis showed that differentially expressed circRNAs were involved in the pathogenesis of gout via various pathways. Moreover, the expression levels of hsa_circRNA_103657 and hsa_circRNA_000241 were significantly higher in the gout group than those in the HC group, and both correlated significantly with lipid metabolism parameters. Furthermore, the area under the curve of hsa_circRNA_103657 was 0.801 (95% confidence interval (CI): 0.730–0.871; p < 0.001). Our results provide novel insights into the pathogenesis of primary gout. Differentially expressed circRNAs were identified in the PBMCs of gout patients, and these differential circRNAs may play important roles in the development and progression of gout. |
| first_indexed | 2024-12-16T09:01:12Z |
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| id | doaj.art-4eaea5d6c3be454381b73786061e9dbc |
| institution | Directory Open Access Journal |
| issn | 1664-8021 |
| language | English |
| last_indexed | 2024-12-16T09:01:12Z |
| publishDate | 2021-10-01 |
| publisher | Frontiers Media S.A. |
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| series | Frontiers in Genetics |
| spelling | doaj.art-4eaea5d6c3be454381b73786061e9dbc2022-12-21T22:37:10ZengFrontiers Media S.A.Frontiers in Genetics1664-80212021-10-011210.3389/fgene.2021.728091728091Expression Profile and Potential Function of Circular RNAs in Peripheral Blood Mononuclear Cells in Male Patients With Primary GoutFei Dai0Fei Dai1Quan-Bo Zhang2Quan-Bo Zhang3Yi-Ping Tang4Yi-Ping Tang5Yi-Xi He6Yi-Xi He7Ting Yi8Ting Yi9Yu-Feng Qing10Yu-Feng Qing11Research Center of Hyperuricemia and Gout, Affiliated Hospital of North Sichuan Medical College, North Sichuan Medical College, Nanchong, ChinaDepartment of Rheumatology and Immunology, Affiliated Hospital of North Sichuan Medical College, North Sichuan Medical College, Nanchong, ChinaResearch Center of Hyperuricemia and Gout, Affiliated Hospital of North Sichuan Medical College, North Sichuan Medical College, Nanchong, ChinaDepartment of Geriatrics, Affiliated Hospital of North Sichuan Medical College, North Sichuan Medical College, Nanchong, ChinaResearch Center of Hyperuricemia and Gout, Affiliated Hospital of North Sichuan Medical College, North Sichuan Medical College, Nanchong, ChinaDepartment of Rheumatology and Immunology, Affiliated Hospital of North Sichuan Medical College, North Sichuan Medical College, Nanchong, ChinaResearch Center of Hyperuricemia and Gout, Affiliated Hospital of North Sichuan Medical College, North Sichuan Medical College, Nanchong, ChinaDepartment of Rheumatology and Immunology, Affiliated Hospital of North Sichuan Medical College, North Sichuan Medical College, Nanchong, ChinaResearch Center of Hyperuricemia and Gout, Affiliated Hospital of North Sichuan Medical College, North Sichuan Medical College, Nanchong, ChinaDepartment of Rheumatology and Immunology, Affiliated Hospital of North Sichuan Medical College, North Sichuan Medical College, Nanchong, ChinaResearch Center of Hyperuricemia and Gout, Affiliated Hospital of North Sichuan Medical College, North Sichuan Medical College, Nanchong, ChinaDepartment of Rheumatology and Immunology, Affiliated Hospital of North Sichuan Medical College, North Sichuan Medical College, Nanchong, ChinaCircular RNAs (circRNAs) are non-coding RNAs (ncRNAs) with a single-stranded covalently closed-loop structure, and their abnormal expression may participate in the pathogenesis of various human diseases. Currently, knowledge of circRNAs in gout is limited. In this case-control study, human circRNA microarrays were used to identify differentially expressed circRNAs in peripheral blood mononuclear cells (PBMCs) from patients with primary gout (n = 5) and healthy controls (HC; n = 3). Bioinformatics methods were used to analyze significantly different circRNAs (fold change >1.5, p < 0.05). In addition, four significantly differentially expressed circRNAs were selected for quantitative real-time polymerase chain reaction to detect expression levels in 90 gout patients and 60 HC. Subsequently, circRNA-miRNA-mRNA network was established to predict the function of circRNAs of interest. Microarray analysis indicated that 238 circRNAs were upregulated and 41 circRNAs were down-regulated in the gout group (fold change >1.5, p < 0.05). Bioinformatics analysis showed that differentially expressed circRNAs were involved in the pathogenesis of gout via various pathways. Moreover, the expression levels of hsa_circRNA_103657 and hsa_circRNA_000241 were significantly higher in the gout group than those in the HC group, and both correlated significantly with lipid metabolism parameters. Furthermore, the area under the curve of hsa_circRNA_103657 was 0.801 (95% confidence interval (CI): 0.730–0.871; p < 0.001). Our results provide novel insights into the pathogenesis of primary gout. Differentially expressed circRNAs were identified in the PBMCs of gout patients, and these differential circRNAs may play important roles in the development and progression of gout.https://www.frontiersin.org/articles/10.3389/fgene.2021.728091/fullgoutcircular RNAmicroarray analysisperipheral blood mononuclear cellsbiomarker |
| spellingShingle | Fei Dai Fei Dai Quan-Bo Zhang Quan-Bo Zhang Yi-Ping Tang Yi-Ping Tang Yi-Xi He Yi-Xi He Ting Yi Ting Yi Yu-Feng Qing Yu-Feng Qing Expression Profile and Potential Function of Circular RNAs in Peripheral Blood Mononuclear Cells in Male Patients With Primary Gout Frontiers in Genetics gout circular RNA microarray analysis peripheral blood mononuclear cells biomarker |
| title | Expression Profile and Potential Function of Circular RNAs in Peripheral Blood Mononuclear Cells in Male Patients With Primary Gout |
| title_full | Expression Profile and Potential Function of Circular RNAs in Peripheral Blood Mononuclear Cells in Male Patients With Primary Gout |
| title_fullStr | Expression Profile and Potential Function of Circular RNAs in Peripheral Blood Mononuclear Cells in Male Patients With Primary Gout |
| title_full_unstemmed | Expression Profile and Potential Function of Circular RNAs in Peripheral Blood Mononuclear Cells in Male Patients With Primary Gout |
| title_short | Expression Profile and Potential Function of Circular RNAs in Peripheral Blood Mononuclear Cells in Male Patients With Primary Gout |
| title_sort | expression profile and potential function of circular rnas in peripheral blood mononuclear cells in male patients with primary gout |
| topic | gout circular RNA microarray analysis peripheral blood mononuclear cells biomarker |
| url | https://www.frontiersin.org/articles/10.3389/fgene.2021.728091/full |
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