A proteomic perspective and involvement of cytokines in SARS-CoV-2 infection.
Infection with the SARS-CoV-2 virus results in manifestation of several clinical observations from asymptomatic to multi-organ failure. Biochemically, the serious effects are due to what is described as cytokine storm. The initial infection region for COVID-19 is the nasopharyngeal/oropharyngeal reg...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2023-01-01
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| Series: | PLoS ONE |
| Online Access: | https://doi.org/10.1371/journal.pone.0279998 |
| _version_ | 1797866023611793408 |
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| author | Sarena Banu Ramakrishnan Nagaraj Mohammed M Idris |
| author_facet | Sarena Banu Ramakrishnan Nagaraj Mohammed M Idris |
| author_sort | Sarena Banu |
| collection | DOAJ |
| description | Infection with the SARS-CoV-2 virus results in manifestation of several clinical observations from asymptomatic to multi-organ failure. Biochemically, the serious effects are due to what is described as cytokine storm. The initial infection region for COVID-19 is the nasopharyngeal/oropharyngeal region which is the site where samples are taken to examine the presence of virus. We have now carried out detailed proteomic analysis of the nasopharyngeal/oropharyngeal swab samples collected from normal individuals and those tested positive for SARS-CoV-2, in India, during the early days of the pandemic in 2020, by RTPCR, involving high throughput quantitative proteomics analysis. Several proteins like annexins, cytokines and histones were found differentially regulated in the host human cells following SARS-CoV-2 infection. Genes for these proteins were also observed to be differentially regulated when their expression was analyzed. Majority of the cytokine proteins were found to be up regulated in the infected individuals. Cell to Cell signaling interaction, Immune cell trafficking and inflammatory response pathways were found associated with the differentially regulated proteins based on network pathway analysis. |
| first_indexed | 2024-04-09T23:17:25Z |
| format | Article |
| id | doaj.art-4eafa1582c3f4364b31203aca6155fa4 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-04-09T23:17:25Z |
| publishDate | 2023-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-4eafa1582c3f4364b31203aca6155fa42023-03-22T05:31:22ZengPublic Library of Science (PLoS)PLoS ONE1932-62032023-01-01181e027999810.1371/journal.pone.0279998A proteomic perspective and involvement of cytokines in SARS-CoV-2 infection.Sarena BanuRamakrishnan NagarajMohammed M IdrisInfection with the SARS-CoV-2 virus results in manifestation of several clinical observations from asymptomatic to multi-organ failure. Biochemically, the serious effects are due to what is described as cytokine storm. The initial infection region for COVID-19 is the nasopharyngeal/oropharyngeal region which is the site where samples are taken to examine the presence of virus. We have now carried out detailed proteomic analysis of the nasopharyngeal/oropharyngeal swab samples collected from normal individuals and those tested positive for SARS-CoV-2, in India, during the early days of the pandemic in 2020, by RTPCR, involving high throughput quantitative proteomics analysis. Several proteins like annexins, cytokines and histones were found differentially regulated in the host human cells following SARS-CoV-2 infection. Genes for these proteins were also observed to be differentially regulated when their expression was analyzed. Majority of the cytokine proteins were found to be up regulated in the infected individuals. Cell to Cell signaling interaction, Immune cell trafficking and inflammatory response pathways were found associated with the differentially regulated proteins based on network pathway analysis.https://doi.org/10.1371/journal.pone.0279998 |
| spellingShingle | Sarena Banu Ramakrishnan Nagaraj Mohammed M Idris A proteomic perspective and involvement of cytokines in SARS-CoV-2 infection. PLoS ONE |
| title | A proteomic perspective and involvement of cytokines in SARS-CoV-2 infection. |
| title_full | A proteomic perspective and involvement of cytokines in SARS-CoV-2 infection. |
| title_fullStr | A proteomic perspective and involvement of cytokines in SARS-CoV-2 infection. |
| title_full_unstemmed | A proteomic perspective and involvement of cytokines in SARS-CoV-2 infection. |
| title_short | A proteomic perspective and involvement of cytokines in SARS-CoV-2 infection. |
| title_sort | proteomic perspective and involvement of cytokines in sars cov 2 infection |
| url | https://doi.org/10.1371/journal.pone.0279998 |
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