Transcriptomic Analysis of Oryza sativa Leaves Reveals Key Changes in Response to Magnaporthe oryzae MSP1
Rice blast disease, caused by Magnaporthe oryzae, results in an extensive loss of rice productivity. Previously, we identified a novel M. oryzae secreted protein, termed MSP1 which causes cell death and pathogen-associated molecular pattern (PAMP)-triggered immune (PTI) responses in rice. Here, we r...
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| Format: | Article |
| Language: | English |
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Hanrimwon Publishing Company
2018-08-01
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| Series: | The Plant Pathology Journal |
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| Online Access: | http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6097817 |
| _version_ | 1819078653788553216 |
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| author | Qingfeng Meng Ravi Gupta Soon Jae Kwon Yiming Wang Ganesh Kumar Agrawal Randeep Rakwal Sang-Ryeol Park Sun Tae Kim |
| author_facet | Qingfeng Meng Ravi Gupta Soon Jae Kwon Yiming Wang Ganesh Kumar Agrawal Randeep Rakwal Sang-Ryeol Park Sun Tae Kim |
| author_sort | Qingfeng Meng |
| collection | DOAJ |
| description | Rice blast disease, caused by Magnaporthe oryzae, results in an extensive loss of rice productivity. Previously, we identified a novel M. oryzae secreted protein, termed MSP1 which causes cell death and pathogen-associated molecular pattern (PAMP)-triggered immune (PTI) responses in rice. Here, we report the transcriptome profile of MSP1-induced response in rice, which led to the identification of 21,619 genes, among which 4,386 showed significant changes (P < 0.05 and fold change > 2 or < 1/2) in response to exogenous MSP1 treatment. Functional annotation of differentially regulated genes showed that the suppressed genes were deeply associated with photosynthesis, secondary metabolism, lipid synthesis, and protein synthesis, while the induced genes were involved in lipid degradation, protein degradation, and signaling. Moreover, expression of genes encoding receptor-like kinases, MAPKs, WRKYs, hormone signaling proteins and pathogenesis-related (PR) proteins were also induced by MSP1. Mapping these differentially expressed genes onto various pathways revealed critical information about the MSP1-triggered responses, providing new insights into the molecular mechanism and components of MSP1-triggered PTI responses in rice. |
| first_indexed | 2024-12-21T19:16:31Z |
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| id | doaj.art-4eb0ec4a6c834cb984af2ffd070c0beb |
| institution | Directory Open Access Journal |
| issn | 1598-2254 |
| language | English |
| last_indexed | 2024-12-21T19:16:31Z |
| publishDate | 2018-08-01 |
| publisher | Hanrimwon Publishing Company |
| record_format | Article |
| series | The Plant Pathology Journal |
| spelling | doaj.art-4eb0ec4a6c834cb984af2ffd070c0beb2022-12-21T18:53:03ZengHanrimwon Publishing CompanyThe Plant Pathology Journal1598-22542018-08-0134425726810.5423/PPJ.OA.01.2018.000810.5423PPJ.OA.01.2018.0008Transcriptomic Analysis of Oryza sativa Leaves Reveals Key Changes in Response to Magnaporthe oryzae MSP1Qingfeng Meng0Ravi Gupta1Soon Jae Kwon2Yiming Wang3Ganesh Kumar Agrawal4Randeep Rakwal5Sang-Ryeol Park6Sun Tae Kim7Department of Plant Bioscience, Life and Energy Convergence Research Institute, Pusan National University, Miryang 46241, KoreaDepartment of Plant Bioscience, Life and Energy Convergence Research Institute, Pusan National University, Miryang 46241, KoreaDepartment of Plant Bioscience, Life and Energy Convergence Research Institute, Pusan National University, Miryang 46241, KoreaDepartment of Plant-Microbe Interactions, Max Planck Institute for Plant Breeding Research, Carl-von-Linne Weg 10, 50829 Cologne, GermanyResearch Laboratory for Biotechnology and Biochemistry (RLABB), GPO Box 13265, Kathmandu, NepalResearch Laboratory for Biotechnology and Biochemistry (RLABB), GPO Box 13265, Kathmandu, NepalGene Engineering Division, National Institute of Agricultural Sciences, Rural Development Administration, Jeonju 54874, KoreaDepartment of Plant Bioscience, Life and Energy Convergence Research Institute, Pusan National University, Miryang 46241, KoreaRice blast disease, caused by Magnaporthe oryzae, results in an extensive loss of rice productivity. Previously, we identified a novel M. oryzae secreted protein, termed MSP1 which causes cell death and pathogen-associated molecular pattern (PAMP)-triggered immune (PTI) responses in rice. Here, we report the transcriptome profile of MSP1-induced response in rice, which led to the identification of 21,619 genes, among which 4,386 showed significant changes (P < 0.05 and fold change > 2 or < 1/2) in response to exogenous MSP1 treatment. Functional annotation of differentially regulated genes showed that the suppressed genes were deeply associated with photosynthesis, secondary metabolism, lipid synthesis, and protein synthesis, while the induced genes were involved in lipid degradation, protein degradation, and signaling. Moreover, expression of genes encoding receptor-like kinases, MAPKs, WRKYs, hormone signaling proteins and pathogenesis-related (PR) proteins were also induced by MSP1. Mapping these differentially expressed genes onto various pathways revealed critical information about the MSP1-triggered responses, providing new insights into the molecular mechanism and components of MSP1-triggered PTI responses in rice.http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6097817DNA microarrayMagnaporthe oryzaeMSP1PAMP-triggered immunityrice |
| spellingShingle | Qingfeng Meng Ravi Gupta Soon Jae Kwon Yiming Wang Ganesh Kumar Agrawal Randeep Rakwal Sang-Ryeol Park Sun Tae Kim Transcriptomic Analysis of Oryza sativa Leaves Reveals Key Changes in Response to Magnaporthe oryzae MSP1 The Plant Pathology Journal DNA microarray Magnaporthe oryzae MSP1 PAMP-triggered immunity rice |
| title | Transcriptomic Analysis of Oryza sativa Leaves Reveals Key Changes in Response to Magnaporthe oryzae MSP1 |
| title_full | Transcriptomic Analysis of Oryza sativa Leaves Reveals Key Changes in Response to Magnaporthe oryzae MSP1 |
| title_fullStr | Transcriptomic Analysis of Oryza sativa Leaves Reveals Key Changes in Response to Magnaporthe oryzae MSP1 |
| title_full_unstemmed | Transcriptomic Analysis of Oryza sativa Leaves Reveals Key Changes in Response to Magnaporthe oryzae MSP1 |
| title_short | Transcriptomic Analysis of Oryza sativa Leaves Reveals Key Changes in Response to Magnaporthe oryzae MSP1 |
| title_sort | transcriptomic analysis of oryza sativa leaves reveals key changes in response to magnaporthe oryzae msp1 |
| topic | DNA microarray Magnaporthe oryzae MSP1 PAMP-triggered immunity rice |
| url | http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6097817 |
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