Dietary Betaine Attenuates High-Carbohydrate-Diet-Induced Oxidative Stress, Endoplasmic Reticulum Stress, and Apoptosis in Mandarin Fish (<i>Siniperca chuatsi</i>)
To investigate the impact of betaine on high-carbohydrate-diet-induced oxidative stress and endoplasmic reticulum (ER) stress, mandarin fish (<i>Siniperca chuatsi</i>) (23.73 ± 0.05 g) were fed with control (NC), betaine (BET), high carbohydrate (HC), and high carbohydrate + betaine (HC...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-10-01
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| Series: | Antioxidants |
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| Online Access: | https://www.mdpi.com/2076-3921/12/10/1860 |
| _version_ | 1797574875221590016 |
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| author | Hongyan Li Yanzhi Zeng Xinyu Zheng Guangjun Wang Jingjing Tian Wangbao Gong Yun Xia Kai Zhang Zhifei Li Wenping Xie Jun Xie Ermeng Yu |
| author_facet | Hongyan Li Yanzhi Zeng Xinyu Zheng Guangjun Wang Jingjing Tian Wangbao Gong Yun Xia Kai Zhang Zhifei Li Wenping Xie Jun Xie Ermeng Yu |
| author_sort | Hongyan Li |
| collection | DOAJ |
| description | To investigate the impact of betaine on high-carbohydrate-diet-induced oxidative stress and endoplasmic reticulum (ER) stress, mandarin fish (<i>Siniperca chuatsi</i>) (23.73 ± 0.05 g) were fed with control (NC), betaine (BET), high carbohydrate (HC), and high carbohydrate + betaine (HC + BET) diets for 8 weeks. The results showed that betaine significantly promoted the growth of mandarin fish irrespective of the dietary carbohydrate levels. The HC diet induced oxidative stress, as evidenced by significantly elevated MDA levels. The HC diet significantly stimulated the mRNA levels of genes involved in ER stress (<i>ire1</i>, <i>perk</i>, <i>atf6</i>, <i>xbp1</i>, <i>eif2α</i>, <i>atf4</i>, <i>chop</i>), autophagy (<i>ulk1</i>, <i>becn1</i>, <i>lc3b</i>), and apoptosis (<i>bax</i>). However, betaine mitigated HC-diet-induced oxidative stress by modulating antioxidant enzymes and alleviated ER stress by regulating the mRNA of genes in the PERK-eIF2a-ATF4 pathway. Additionally, betaine significantly reduced the mRNA levels of <i>becn1</i> and <i>bax</i>, along with the apoptosis rate, indicating a mitigating effect on autophagy and apoptosis. Overall, dietary betaine improved growth, attenuated HC-diet-induced oxidative stress and ER stress, and ultimately alleviated apoptosis in mandarin fish. These findings provide evidence for the use of betaine in aquafeeds to counter disruptive effects due to diets containing high carbohydrate levels. |
| first_indexed | 2024-03-10T21:29:18Z |
| format | Article |
| id | doaj.art-4eb27ea243344e0ca59040d12b1f2639 |
| institution | Directory Open Access Journal |
| issn | 2076-3921 |
| language | English |
| last_indexed | 2024-03-10T21:29:18Z |
| publishDate | 2023-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Antioxidants |
| spelling | doaj.art-4eb27ea243344e0ca59040d12b1f26392023-11-19T15:28:12ZengMDPI AGAntioxidants2076-39212023-10-011210186010.3390/antiox12101860Dietary Betaine Attenuates High-Carbohydrate-Diet-Induced Oxidative Stress, Endoplasmic Reticulum Stress, and Apoptosis in Mandarin Fish (<i>Siniperca chuatsi</i>)Hongyan Li0Yanzhi Zeng1Xinyu Zheng2Guangjun Wang3Jingjing Tian4Wangbao Gong5Yun Xia6Kai Zhang7Zhifei Li8Wenping Xie9Jun Xie10Ermeng Yu11Key Laboratory of Tropical & Subtropical Fishery Resource Application & Cultivation, Pearl River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou 510380, ChinaKey Laboratory of Tropical & Subtropical Fishery Resource Application & Cultivation, Pearl River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou 510380, ChinaKey Laboratory of Tropical & Subtropical Fishery Resource Application & Cultivation, Pearl River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou 510380, ChinaKey Laboratory of Tropical & Subtropical Fishery Resource Application & Cultivation, Pearl River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou 510380, ChinaKey Laboratory of Tropical & Subtropical Fishery Resource Application & Cultivation, Pearl River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou 510380, ChinaKey Laboratory of Tropical & Subtropical Fishery Resource Application & Cultivation, Pearl River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou 510380, ChinaKey Laboratory of Tropical & Subtropical Fishery Resource Application & Cultivation, Pearl River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou 510380, ChinaKey Laboratory of Tropical & Subtropical Fishery Resource Application & Cultivation, Pearl River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou 510380, ChinaKey Laboratory of Tropical & Subtropical Fishery Resource Application & Cultivation, Pearl River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou 510380, ChinaKey Laboratory of Tropical & Subtropical Fishery Resource Application & Cultivation, Pearl River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou 510380, ChinaKey Laboratory of Tropical & Subtropical Fishery Resource Application & Cultivation, Pearl River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou 510380, ChinaKey Laboratory of Tropical & Subtropical Fishery Resource Application & Cultivation, Pearl River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou 510380, ChinaTo investigate the impact of betaine on high-carbohydrate-diet-induced oxidative stress and endoplasmic reticulum (ER) stress, mandarin fish (<i>Siniperca chuatsi</i>) (23.73 ± 0.05 g) were fed with control (NC), betaine (BET), high carbohydrate (HC), and high carbohydrate + betaine (HC + BET) diets for 8 weeks. The results showed that betaine significantly promoted the growth of mandarin fish irrespective of the dietary carbohydrate levels. The HC diet induced oxidative stress, as evidenced by significantly elevated MDA levels. The HC diet significantly stimulated the mRNA levels of genes involved in ER stress (<i>ire1</i>, <i>perk</i>, <i>atf6</i>, <i>xbp1</i>, <i>eif2α</i>, <i>atf4</i>, <i>chop</i>), autophagy (<i>ulk1</i>, <i>becn1</i>, <i>lc3b</i>), and apoptosis (<i>bax</i>). However, betaine mitigated HC-diet-induced oxidative stress by modulating antioxidant enzymes and alleviated ER stress by regulating the mRNA of genes in the PERK-eIF2a-ATF4 pathway. Additionally, betaine significantly reduced the mRNA levels of <i>becn1</i> and <i>bax</i>, along with the apoptosis rate, indicating a mitigating effect on autophagy and apoptosis. Overall, dietary betaine improved growth, attenuated HC-diet-induced oxidative stress and ER stress, and ultimately alleviated apoptosis in mandarin fish. These findings provide evidence for the use of betaine in aquafeeds to counter disruptive effects due to diets containing high carbohydrate levels.https://www.mdpi.com/2076-3921/12/10/1860mandarin fishhigh carbohydrate dietbetaineoxidative stressER stress |
| spellingShingle | Hongyan Li Yanzhi Zeng Xinyu Zheng Guangjun Wang Jingjing Tian Wangbao Gong Yun Xia Kai Zhang Zhifei Li Wenping Xie Jun Xie Ermeng Yu Dietary Betaine Attenuates High-Carbohydrate-Diet-Induced Oxidative Stress, Endoplasmic Reticulum Stress, and Apoptosis in Mandarin Fish (<i>Siniperca chuatsi</i>) Antioxidants mandarin fish high carbohydrate diet betaine oxidative stress ER stress |
| title | Dietary Betaine Attenuates High-Carbohydrate-Diet-Induced Oxidative Stress, Endoplasmic Reticulum Stress, and Apoptosis in Mandarin Fish (<i>Siniperca chuatsi</i>) |
| title_full | Dietary Betaine Attenuates High-Carbohydrate-Diet-Induced Oxidative Stress, Endoplasmic Reticulum Stress, and Apoptosis in Mandarin Fish (<i>Siniperca chuatsi</i>) |
| title_fullStr | Dietary Betaine Attenuates High-Carbohydrate-Diet-Induced Oxidative Stress, Endoplasmic Reticulum Stress, and Apoptosis in Mandarin Fish (<i>Siniperca chuatsi</i>) |
| title_full_unstemmed | Dietary Betaine Attenuates High-Carbohydrate-Diet-Induced Oxidative Stress, Endoplasmic Reticulum Stress, and Apoptosis in Mandarin Fish (<i>Siniperca chuatsi</i>) |
| title_short | Dietary Betaine Attenuates High-Carbohydrate-Diet-Induced Oxidative Stress, Endoplasmic Reticulum Stress, and Apoptosis in Mandarin Fish (<i>Siniperca chuatsi</i>) |
| title_sort | dietary betaine attenuates high carbohydrate diet induced oxidative stress endoplasmic reticulum stress and apoptosis in mandarin fish i siniperca chuatsi i |
| topic | mandarin fish high carbohydrate diet betaine oxidative stress ER stress |
| url | https://www.mdpi.com/2076-3921/12/10/1860 |
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