Activation of Invariant Natural Killer T Cell Subsets in C57BL/6J Mice by Different Injection Modes of α-galactosylceramide
Whether different injection modes of α-galactosylceramide (α-GalCer) affect the activation of different subsets of invariant natural killer T (iNKT) cells in different tissues and organs of mice is unclear. This study included healthy control, subcutaneous injection, and intraperitoneal injection gr...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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Tehran University of Medical Sciences
2020-02-01
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| Series: | Iranian Journal of Allergy, Asthma and Immunology |
| Subjects: | |
| Online Access: | https://ijaai.tums.ac.ir/index.php/ijaai/article/view/2346 |
| _version_ | 1818127207821737984 |
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| author | Ming Meng Shengde Chen Xiang Gao Huifang Liu Huijuan Zhao Jingnan Zhang Jinku Zhang Dongzhi Chen |
| author_facet | Ming Meng Shengde Chen Xiang Gao Huifang Liu Huijuan Zhao Jingnan Zhang Jinku Zhang Dongzhi Chen |
| author_sort | Ming Meng |
| collection | DOAJ |
| description | Whether different injection modes of α-galactosylceramide (α-GalCer) affect the activation of different subsets of invariant natural killer T (iNKT) cells in different tissues and organs of mice is unclear. This study included healthy control, subcutaneous injection, and intraperitoneal injection groups (n=10 in each group). The subcutaneous and intraperitoneal injection groups were injected with α-Galcer (0.1 mg/kg weight), and then the changes in thymus, spleen, and liver iNKT cell frequencies and subsets were observed. The intraperitoneal injection of α-GalCer could increase the frequency of splenic iNKT cells, but the subcutaneous injection did not affect the frequency. Neither injection had any effect on the frequency of iNKT cells in the thymus and liver. The subcutaneous injection of α-GalCer increased the rate of iNKT2 subsets in the thymus but did not affect the rate of iNKT1 subsets. However, the intraperitoneal injection of α-GalCer did not affect thymus iNKT1 and iNKT2 subsets. Interestingly, the subcutaneous injection of α-GalCer significantly increased the proportion of iNKT1 in the spleen and liver but did not significantly change the proportion of iNKT2. The intraperitoneal injection of α-GalCer significantly increased the rate of iNKT2 in spleen and liver but decreased the rate of iNKT1. Subsets of iNKT1 or iNKT2 cells in the spleen and liver were selectively activated by the subcutaneous or intraperitoneal injection of α-GalCer. It provides a valuable means for treating tumors and certain autoimmune diseases. Further exploration of the activation mechanism may provide new ideas about the development of related vaccines. |
| first_indexed | 2024-12-11T07:13:42Z |
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| id | doaj.art-4eb524c5b5ae43c4926c49776c9efa1b |
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| issn | 1735-1502 1735-5249 |
| language | English |
| last_indexed | 2024-12-11T07:13:42Z |
| publishDate | 2020-02-01 |
| publisher | Tehran University of Medical Sciences |
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| series | Iranian Journal of Allergy, Asthma and Immunology |
| spelling | doaj.art-4eb524c5b5ae43c4926c49776c9efa1b2022-12-22T01:16:18ZengTehran University of Medical SciencesIranian Journal of Allergy, Asthma and Immunology1735-15021735-52492020-02-0119110.18502/ijaai.v19i1.24162346Activation of Invariant Natural Killer T Cell Subsets in C57BL/6J Mice by Different Injection Modes of α-galactosylceramideMing Meng0Shengde Chen1Xiang Gao2Huifang Liu3Huijuan Zhao4Jingnan Zhang5Jinku Zhang6Dongzhi Chen7Medical School of Hebei University, Baoding, Hebei Province, China AND Key Laboratory of Pathogenesis Mechanism and Control of Inflammatory-autoimmune Diseases in Hebei Province, Baoding, ChinaMedical School of Hebei University, Baoding, Hebei Province, China AND Key Laboratory of Pathogenesis Mechanism and Control of Inflammatory-autoimmune Diseases in Hebei Province, Baoding, ChinaMedical School of Hebei University, Baoding, Hebei Province, China AND Key Laboratory of Pathogenesis Mechanism and Control of Inflammatory-autoimmune Diseases in Hebei Province, Baoding, ChinaMedical School of Hebei University, Baoding, Hebei Province, China AND Key Laboratory of Pathogenesis Mechanism and Control of Inflammatory-autoimmune Diseases in Hebei Province, Baoding, ChinaMedical School of Hebei University, Baoding, Hebei Province, China AND Key Laboratory of Pathogenesis Mechanism and Control of Inflammatory-autoimmune Diseases in Hebei Province, Baoding, ChinaDepartment of Pathology, The First Centre Hospital of Baoding, Baoding, Hebei Province, ChinaMedical School of Hebei University, Baoding, Hebei Province, China AND Key Laboratory of Pathogenesis Mechanism and Control of Inflammatory-autoimmune Diseases in Hebei Province, Baoding, ChinaMedical School of Hebei University, Baoding, Hebei Province, China AND Key Laboratory of Pathogenesis Mechanism and Control of Inflammatory-autoimmune Diseases in Hebei Province, Baoding, ChinaWhether different injection modes of α-galactosylceramide (α-GalCer) affect the activation of different subsets of invariant natural killer T (iNKT) cells in different tissues and organs of mice is unclear. This study included healthy control, subcutaneous injection, and intraperitoneal injection groups (n=10 in each group). The subcutaneous and intraperitoneal injection groups were injected with α-Galcer (0.1 mg/kg weight), and then the changes in thymus, spleen, and liver iNKT cell frequencies and subsets were observed. The intraperitoneal injection of α-GalCer could increase the frequency of splenic iNKT cells, but the subcutaneous injection did not affect the frequency. Neither injection had any effect on the frequency of iNKT cells in the thymus and liver. The subcutaneous injection of α-GalCer increased the rate of iNKT2 subsets in the thymus but did not affect the rate of iNKT1 subsets. However, the intraperitoneal injection of α-GalCer did not affect thymus iNKT1 and iNKT2 subsets. Interestingly, the subcutaneous injection of α-GalCer significantly increased the proportion of iNKT1 in the spleen and liver but did not significantly change the proportion of iNKT2. The intraperitoneal injection of α-GalCer significantly increased the rate of iNKT2 in spleen and liver but decreased the rate of iNKT1. Subsets of iNKT1 or iNKT2 cells in the spleen and liver were selectively activated by the subcutaneous or intraperitoneal injection of α-GalCer. It provides a valuable means for treating tumors and certain autoimmune diseases. Further exploration of the activation mechanism may provide new ideas about the development of related vaccines.https://ijaai.tums.ac.ir/index.php/ijaai/article/view/2346CytokinesInvariant natural killer T cellsIntraperitoneal injectionSubcutaneous injectionα-galactosylceramide |
| spellingShingle | Ming Meng Shengde Chen Xiang Gao Huifang Liu Huijuan Zhao Jingnan Zhang Jinku Zhang Dongzhi Chen Activation of Invariant Natural Killer T Cell Subsets in C57BL/6J Mice by Different Injection Modes of α-galactosylceramide Iranian Journal of Allergy, Asthma and Immunology Cytokines Invariant natural killer T cells Intraperitoneal injection Subcutaneous injection α-galactosylceramide |
| title | Activation of Invariant Natural Killer T Cell Subsets in C57BL/6J Mice by Different Injection Modes of α-galactosylceramide |
| title_full | Activation of Invariant Natural Killer T Cell Subsets in C57BL/6J Mice by Different Injection Modes of α-galactosylceramide |
| title_fullStr | Activation of Invariant Natural Killer T Cell Subsets in C57BL/6J Mice by Different Injection Modes of α-galactosylceramide |
| title_full_unstemmed | Activation of Invariant Natural Killer T Cell Subsets in C57BL/6J Mice by Different Injection Modes of α-galactosylceramide |
| title_short | Activation of Invariant Natural Killer T Cell Subsets in C57BL/6J Mice by Different Injection Modes of α-galactosylceramide |
| title_sort | activation of invariant natural killer t cell subsets in c57bl 6j mice by different injection modes of α galactosylceramide |
| topic | Cytokines Invariant natural killer T cells Intraperitoneal injection Subcutaneous injection α-galactosylceramide |
| url | https://ijaai.tums.ac.ir/index.php/ijaai/article/view/2346 |
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