17β-estradiol lowers triglycerides in adipocytes via estrogen receptor α and it may be attenuated by inflammation
Abstract Background Estrogen was reported to protect against obesity, however the mechanism remains unclear. We aimed to investigate the impact of 17β-estradiol (17β-E2) on triglyceride metabolism in adipocytes with or without lipopolysacchride (LPS) stimulating, providing novel potential mechanism...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2017-09-01
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| Series: | Lipids in Health and Disease |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s12944-017-0575-6 |
| _version_ | 1819263811319758848 |
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| author | Fei Luo Wen-yu Huang Yuan Guo Gui-yun Ruan Ran Peng Xiang-ping Li |
| author_facet | Fei Luo Wen-yu Huang Yuan Guo Gui-yun Ruan Ran Peng Xiang-ping Li |
| author_sort | Fei Luo |
| collection | DOAJ |
| description | Abstract Background Estrogen was reported to protect against obesity, however the mechanism remains unclear. We aimed to investigate the impact of 17β-estradiol (17β-E2) on triglyceride metabolism in adipocytes with or without lipopolysacchride (LPS) stimulating, providing novel potential mechanism for estrogen action. Methods 3T3-L1 adipocytes were cultured and differentiated into mature adipocytes in vitro. The differentiated 3T3-L1 cells were divided into six groups: (i) control group, treated with 0.1% DMSO alone; (ii) 17β-E2 group, treated with 1, 0.1, or 0.001 μM 17β-E2 for 48 h; (iii) 17β-E2 plus MPP group, pre-treated with 10 μM MPP (a selective ERα receptor inhibitor) for 1 h, then incubated with 1 μM 17β-E2 for 48 h; (iv) 17β-E2 plus PHTPP group, pre-treated with 10 μM PHTPP (a selective ERβ receptor inhibitor), then incubated with 1 μM 17β-E2 for 48 h; (v) LPS group, pre-treated with 100 ng/mL LPS for 24 h, then cells were washed by PBS for 3 times and incubated with 0.1% DMSO alone for 48 h; (vi) 17β-E2 plus LPS group, pre-treated with 100 ng/mL LPS for 24 h, then cells were washed by PBS for 3 times and incubated with 1 μM 17β-E2 for 48 h. The levels of triglyceride and adipose triglyceride lipase (ATGL) in differentiated 3T3-L1 cells and the concentrations of interleukin-6 (IL-6) in culture medium were measured. Results Comparing with control group, 1 μM and 0.1 μM 17β-E2 decreased the intracellular TG levels by about 20% and 10% respectively (all P < 0.05). The triglyceride-lowing effect of 17β-E2 in differentiated 3T3-L1 cells was abolished by ERα antagonist MPP but not ERβ antagonist PHTPP. Comparing with control group, the IL-6 levels were significantly higher in the culture medium of the cultured differentiated 3T3-L1 cells in LPS group and 17β-E2 + LPS group (all P < 0.05). And, the IL-6 levels were similar in LPS group and 17β-E2 + LPS group (P > 0.05). There was no significant difference in the triglyceride contents of differentiated 3T3-L1 cells among control group, LPS group and 17β-E2 + LPS group (all P > 0.05). ATGL expression in 17β-E2 group was significantly higher than control group (P < 0.05), which was abolished by ERα antagonist MPP or LPS. Conclusions 17β-E2 increased ATGL expression and lowered triglycerides in adipocytes but not in LPS stimulated adipocytes via estrogen ERα. |
| first_indexed | 2024-12-23T20:19:31Z |
| format | Article |
| id | doaj.art-4eba87668ce24f2cbcb3a426dc47ac3f |
| institution | Directory Open Access Journal |
| issn | 1476-511X |
| language | English |
| last_indexed | 2024-12-23T20:19:31Z |
| publishDate | 2017-09-01 |
| publisher | BMC |
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| series | Lipids in Health and Disease |
| spelling | doaj.art-4eba87668ce24f2cbcb3a426dc47ac3f2022-12-21T17:32:35ZengBMCLipids in Health and Disease1476-511X2017-09-011611610.1186/s12944-017-0575-617β-estradiol lowers triglycerides in adipocytes via estrogen receptor α and it may be attenuated by inflammationFei Luo0Wen-yu Huang1Yuan Guo2Gui-yun Ruan3Ran Peng4Xiang-ping Li5Department of Cardiovascular Medicine, The Second Xiangya Hospital, Central South UniversityDepartment of Emergency Medicine, Yantai Yuhuangding Hospital, Qingdao University Medical CollegeDepartment of Cardiovascular Medicine, The Second Xiangya Hospital, Central South UniversityDepartment of Cardiovascular Medicine, The Second Xiangya Hospital, Central South UniversityDepartment of Cardiovascular Medicine, The Second Xiangya Hospital, Central South UniversityDepartment of Cardiovascular Medicine, The Second Xiangya Hospital, Central South UniversityAbstract Background Estrogen was reported to protect against obesity, however the mechanism remains unclear. We aimed to investigate the impact of 17β-estradiol (17β-E2) on triglyceride metabolism in adipocytes with or without lipopolysacchride (LPS) stimulating, providing novel potential mechanism for estrogen action. Methods 3T3-L1 adipocytes were cultured and differentiated into mature adipocytes in vitro. The differentiated 3T3-L1 cells were divided into six groups: (i) control group, treated with 0.1% DMSO alone; (ii) 17β-E2 group, treated with 1, 0.1, or 0.001 μM 17β-E2 for 48 h; (iii) 17β-E2 plus MPP group, pre-treated with 10 μM MPP (a selective ERα receptor inhibitor) for 1 h, then incubated with 1 μM 17β-E2 for 48 h; (iv) 17β-E2 plus PHTPP group, pre-treated with 10 μM PHTPP (a selective ERβ receptor inhibitor), then incubated with 1 μM 17β-E2 for 48 h; (v) LPS group, pre-treated with 100 ng/mL LPS for 24 h, then cells were washed by PBS for 3 times and incubated with 0.1% DMSO alone for 48 h; (vi) 17β-E2 plus LPS group, pre-treated with 100 ng/mL LPS for 24 h, then cells were washed by PBS for 3 times and incubated with 1 μM 17β-E2 for 48 h. The levels of triglyceride and adipose triglyceride lipase (ATGL) in differentiated 3T3-L1 cells and the concentrations of interleukin-6 (IL-6) in culture medium were measured. Results Comparing with control group, 1 μM and 0.1 μM 17β-E2 decreased the intracellular TG levels by about 20% and 10% respectively (all P < 0.05). The triglyceride-lowing effect of 17β-E2 in differentiated 3T3-L1 cells was abolished by ERα antagonist MPP but not ERβ antagonist PHTPP. Comparing with control group, the IL-6 levels were significantly higher in the culture medium of the cultured differentiated 3T3-L1 cells in LPS group and 17β-E2 + LPS group (all P < 0.05). And, the IL-6 levels were similar in LPS group and 17β-E2 + LPS group (P > 0.05). There was no significant difference in the triglyceride contents of differentiated 3T3-L1 cells among control group, LPS group and 17β-E2 + LPS group (all P > 0.05). ATGL expression in 17β-E2 group was significantly higher than control group (P < 0.05), which was abolished by ERα antagonist MPP or LPS. Conclusions 17β-E2 increased ATGL expression and lowered triglycerides in adipocytes but not in LPS stimulated adipocytes via estrogen ERα.http://link.springer.com/article/10.1186/s12944-017-0575-617β-estradiolAdipocyteTriglycerideEstrogen receptor α |
| spellingShingle | Fei Luo Wen-yu Huang Yuan Guo Gui-yun Ruan Ran Peng Xiang-ping Li 17β-estradiol lowers triglycerides in adipocytes via estrogen receptor α and it may be attenuated by inflammation Lipids in Health and Disease 17β-estradiol Adipocyte Triglyceride Estrogen receptor α |
| title | 17β-estradiol lowers triglycerides in adipocytes via estrogen receptor α and it may be attenuated by inflammation |
| title_full | 17β-estradiol lowers triglycerides in adipocytes via estrogen receptor α and it may be attenuated by inflammation |
| title_fullStr | 17β-estradiol lowers triglycerides in adipocytes via estrogen receptor α and it may be attenuated by inflammation |
| title_full_unstemmed | 17β-estradiol lowers triglycerides in adipocytes via estrogen receptor α and it may be attenuated by inflammation |
| title_short | 17β-estradiol lowers triglycerides in adipocytes via estrogen receptor α and it may be attenuated by inflammation |
| title_sort | 17β estradiol lowers triglycerides in adipocytes via estrogen receptor α and it may be attenuated by inflammation |
| topic | 17β-estradiol Adipocyte Triglyceride Estrogen receptor α |
| url | http://link.springer.com/article/10.1186/s12944-017-0575-6 |
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