Suppressing STAT3 activation impairs bone formation during maxillary expansion and relapse
Abstract Objectives The mid-palatal expansion technique is commonly used to correct maxillary constriction in dental clinics. However, there is a tendency for it to relapse, and the key molecules responsible for modulating bone formation remain elusive. Thus, this study aimed to investigate whethe...
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| Format: | Article |
| Language: | English |
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University of São Paulo
2023-05-01
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| Series: | Journal of Applied Oral Science |
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| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-77572023000100419&lng=en&tlng=en |
| _version_ | 1797830839925473280 |
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| author | Xiaoyue XIAO Jianwei CHEN Qiming ZHAI Liangjing XIN Xinhui ZHENG Si WANG Jinlin SONG |
| author_facet | Xiaoyue XIAO Jianwei CHEN Qiming ZHAI Liangjing XIN Xinhui ZHENG Si WANG Jinlin SONG |
| author_sort | Xiaoyue XIAO |
| collection | DOAJ |
| description | Abstract Objectives The mid-palatal expansion technique is commonly used to correct maxillary constriction in dental clinics. However, there is a tendency for it to relapse, and the key molecules responsible for modulating bone formation remain elusive. Thus, this study aimed to investigate whether signal transducer and activator of transcription 3 (STAT3) activation contributes to osteoblast-mediated bone formation during palatal expansion and relapse. Methodology In total, 30 male Wistar rats were randomly allocated into Ctrl (control), E (expansion only), and E+Stattic (expansion plus STAT3-inhibitor, Stattic) groups. Micro-computed tomography, micromorphology staining, and immunohistochemistry of the mid-palatal suture were performed on days 7 and 14. In vitro cyclic tensile stress (10% magnitude, 0.5 Hz frequency, and 24 h duration) was applied to rat primary osteoblasts and Stattic was administered for STAT3 inhibition. The role of STAT3 in mechanical loading-induced osteoblasts was confirmed by alkaline phosphatase (ALP), alizarin red staining, and western blots. Results The E group showed greater arch width than the E+Stattic group after expansion. The differences between the two groups remained significant after relapse. We found active bone formation in the E group with increased expression of ALP, COL-I, and Runx2, although the expression of osteogenesis-related factors was downregulated in the E+stattic group. After STAT3 inhibition, expansive force-induced bone resorption was attenuated, as TRAP staining demonstrated. Furthermore, the administration of Stattic in vitro partially suppressed tensile stress-enhanced osteogenic markers in osteoblasts. Conclusions STAT3 inactivation reduced osteoblast-mediated bone formation during palatal expansion and post-expansion relapse, thus it may be a potential therapeutic target to treat force-induced bone formation. |
| first_indexed | 2024-04-09T13:42:32Z |
| format | Article |
| id | doaj.art-4ebb6ad8a89d471f8b8ac2b6ead2f177 |
| institution | Directory Open Access Journal |
| issn | 1678-7765 |
| language | English |
| last_indexed | 2024-04-09T13:42:32Z |
| publishDate | 2023-05-01 |
| publisher | University of São Paulo |
| record_format | Article |
| series | Journal of Applied Oral Science |
| spelling | doaj.art-4ebb6ad8a89d471f8b8ac2b6ead2f1772023-05-09T07:35:22ZengUniversity of São PauloJournal of Applied Oral Science1678-77652023-05-013110.1590/1678-7757-2023-0009Suppressing STAT3 activation impairs bone formation during maxillary expansion and relapseXiaoyue XIAOJianwei CHENQiming ZHAILiangjing XINXinhui ZHENGSi WANGhttps://orcid.org/0000-0003-4155-0810Jinlin SONGhttps://orcid.org/0000-0002-0224-6640Abstract Objectives The mid-palatal expansion technique is commonly used to correct maxillary constriction in dental clinics. However, there is a tendency for it to relapse, and the key molecules responsible for modulating bone formation remain elusive. Thus, this study aimed to investigate whether signal transducer and activator of transcription 3 (STAT3) activation contributes to osteoblast-mediated bone formation during palatal expansion and relapse. Methodology In total, 30 male Wistar rats were randomly allocated into Ctrl (control), E (expansion only), and E+Stattic (expansion plus STAT3-inhibitor, Stattic) groups. Micro-computed tomography, micromorphology staining, and immunohistochemistry of the mid-palatal suture were performed on days 7 and 14. In vitro cyclic tensile stress (10% magnitude, 0.5 Hz frequency, and 24 h duration) was applied to rat primary osteoblasts and Stattic was administered for STAT3 inhibition. The role of STAT3 in mechanical loading-induced osteoblasts was confirmed by alkaline phosphatase (ALP), alizarin red staining, and western blots. Results The E group showed greater arch width than the E+Stattic group after expansion. The differences between the two groups remained significant after relapse. We found active bone formation in the E group with increased expression of ALP, COL-I, and Runx2, although the expression of osteogenesis-related factors was downregulated in the E+stattic group. After STAT3 inhibition, expansive force-induced bone resorption was attenuated, as TRAP staining demonstrated. Furthermore, the administration of Stattic in vitro partially suppressed tensile stress-enhanced osteogenic markers in osteoblasts. Conclusions STAT3 inactivation reduced osteoblast-mediated bone formation during palatal expansion and post-expansion relapse, thus it may be a potential therapeutic target to treat force-induced bone formation.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-77572023000100419&lng=en&tlng=enBone formationMaxillary expansionSTAT3 protein |
| spellingShingle | Xiaoyue XIAO Jianwei CHEN Qiming ZHAI Liangjing XIN Xinhui ZHENG Si WANG Jinlin SONG Suppressing STAT3 activation impairs bone formation during maxillary expansion and relapse Journal of Applied Oral Science Bone formation Maxillary expansion STAT3 protein |
| title | Suppressing STAT3 activation impairs bone formation during maxillary expansion and relapse |
| title_full | Suppressing STAT3 activation impairs bone formation during maxillary expansion and relapse |
| title_fullStr | Suppressing STAT3 activation impairs bone formation during maxillary expansion and relapse |
| title_full_unstemmed | Suppressing STAT3 activation impairs bone formation during maxillary expansion and relapse |
| title_short | Suppressing STAT3 activation impairs bone formation during maxillary expansion and relapse |
| title_sort | suppressing stat3 activation impairs bone formation during maxillary expansion and relapse |
| topic | Bone formation Maxillary expansion STAT3 protein |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-77572023000100419&lng=en&tlng=en |
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