Visualizing the Needle in the Haystack: In Situ Hybridization With Fluorescent Dendrimers
<p>In situ hybridization with 3DNA™ dendrimers is a novel tool for detecting low levels of mRNA in tissue sections and whole embryos. Fluorescently labeled dendrimers were used to identify cells that express mRNA for the skeletal muscle transcription factor MyoD in the early chick em...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
BMC
2004-01-01
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| Series: | Biological Procedures Online |
| Subjects: | |
| Online Access: | http://www.biologicalprocedures.com/bpo/arts/1/84/m84.htm |
| _version_ | 1818757699951656960 |
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| author | Gerhart Jacquelyn Baytion Michael Perlman Jordanna Neely Christine Hearon Bridget Nilsen Thor Getts Robert Kadushin James George-Weinstein Mindy |
| author_facet | Gerhart Jacquelyn Baytion Michael Perlman Jordanna Neely Christine Hearon Bridget Nilsen Thor Getts Robert Kadushin James George-Weinstein Mindy |
| author_sort | Gerhart Jacquelyn |
| collection | DOAJ |
| description | <p>In situ hybridization with 3DNA™ dendrimers is a novel tool for detecting low levels of mRNA in tissue sections and whole embryos. Fluorescently labeled dendrimers were used to identify cells that express mRNA for the skeletal muscle transcription factor MyoD in the early chick embryo. A small population of MyoD mRNA positive cells was found in the epiblast prior to the initiation of gastrulation, two days earlier than previously detected using enzymatic or radiolabeled probes for mRNA. When isolated from the epiblast and placed in culture, the MyoD mRNA positive cells were able to differentiate into skeletal muscle cells. These results demonstrate that DNA dendrimers are sensitive and precise tools for identifying low levels of mRNA in single cells and tissues. |
| first_indexed | 2024-12-18T06:15:06Z |
| format | Article |
| id | doaj.art-4ebd0c155eec4cbe85ba3322a5628258 |
| institution | Directory Open Access Journal |
| issn | 1480-9222 |
| language | English |
| last_indexed | 2024-12-18T06:15:06Z |
| publishDate | 2004-01-01 |
| publisher | BMC |
| record_format | Article |
| series | Biological Procedures Online |
| spelling | doaj.art-4ebd0c155eec4cbe85ba3322a56282582022-12-21T21:18:17ZengBMCBiological Procedures Online1480-92222004-01-016114915610.1251/bpo84Visualizing the Needle in the Haystack: In Situ Hybridization With Fluorescent DendrimersGerhart JacquelynBaytion MichaelPerlman JordannaNeely ChristineHearon BridgetNilsen ThorGetts RobertKadushin JamesGeorge-Weinstein Mindy<p>In situ hybridization with 3DNA™ dendrimers is a novel tool for detecting low levels of mRNA in tissue sections and whole embryos. Fluorescently labeled dendrimers were used to identify cells that express mRNA for the skeletal muscle transcription factor MyoD in the early chick embryo. A small population of MyoD mRNA positive cells was found in the epiblast prior to the initiation of gastrulation, two days earlier than previously detected using enzymatic or radiolabeled probes for mRNA. When isolated from the epiblast and placed in culture, the MyoD mRNA positive cells were able to differentiate into skeletal muscle cells. These results demonstrate that DNA dendrimers are sensitive and precise tools for identifying low levels of mRNA in single cells and tissues.http://www.biologicalprocedures.com/bpo/arts/1/84/m84.htmMyoD proteinIn situ hybridization |
| spellingShingle | Gerhart Jacquelyn Baytion Michael Perlman Jordanna Neely Christine Hearon Bridget Nilsen Thor Getts Robert Kadushin James George-Weinstein Mindy Visualizing the Needle in the Haystack: In Situ Hybridization With Fluorescent Dendrimers Biological Procedures Online MyoD protein In situ hybridization |
| title | Visualizing the Needle in the Haystack: In Situ Hybridization With Fluorescent Dendrimers |
| title_full | Visualizing the Needle in the Haystack: In Situ Hybridization With Fluorescent Dendrimers |
| title_fullStr | Visualizing the Needle in the Haystack: In Situ Hybridization With Fluorescent Dendrimers |
| title_full_unstemmed | Visualizing the Needle in the Haystack: In Situ Hybridization With Fluorescent Dendrimers |
| title_short | Visualizing the Needle in the Haystack: In Situ Hybridization With Fluorescent Dendrimers |
| title_sort | visualizing the needle in the haystack in situ hybridization with fluorescent dendrimers |
| topic | MyoD protein In situ hybridization |
| url | http://www.biologicalprocedures.com/bpo/arts/1/84/m84.htm |
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