Gene expression, immunohistochemical and microarchitectural evaluation of bone formation around two implant surfaces placed in bone defects filled or not with bone substitute material
Abstract Objective The aim of this study is to evaluate through gene expression, immunohistochemical and microtomographic (micro-CT) analysis the response of peri-implant bone tissue around titanium implants with different surface treatments, placed in bone defects filled or not with bone substitute...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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SpringerOpen
2020-12-01
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| Series: | International Journal of Implant Dentistry |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s40729-020-00279-7 |
| _version_ | 1818875810974531584 |
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| author | Guilherme dos Santos Trento Jaqueline Suemi Hassumi Paula Buzo Frigério Ana Paula Farnezi Bassi Roberta Okamoto Marisa Aparecida Cabrini Gabrielli Valfrido Antonio Pereira-Filho |
| author_facet | Guilherme dos Santos Trento Jaqueline Suemi Hassumi Paula Buzo Frigério Ana Paula Farnezi Bassi Roberta Okamoto Marisa Aparecida Cabrini Gabrielli Valfrido Antonio Pereira-Filho |
| author_sort | Guilherme dos Santos Trento |
| collection | DOAJ |
| description | Abstract Objective The aim of this study is to evaluate through gene expression, immunohistochemical and microtomographic (micro-CT) analysis the response of peri-implant bone tissue around titanium implants with different surface treatments, placed in bone defects filled or not with bone substitute materials. In addition, to investigate the hypothesis that porous-hydrophilic surface induces a faster bone formation. Materials and methods Twenty-six animals were divided into two groups according to implant surface treatment. In each tibia, a bone defect was created followed by the placement of one implant. On the left tibia, the defect was filled with blood clot (BC), and on the right tibia, the defect was filled with biphasic hydroxyapatite/β-tricalcium-phosphate (HA/TCP) generating four subgroups: BC-N: bone defect filled with blood clot and porous surface titanium implant installed; BC-A: bone defect filled with blood clot and porous-hydrophilic surface titanium implant installed; HA/TCP-N: bone defect filled with bone substitute material and porous surface titanium implant installed; and HA/TCP-A: bone defect filled with bone substitute material and porous-hydrophilic surface titanium implant installed. The animals were submitted to euthanasia at 15, 30, and 60 days after implant installation. The expression of two genes was evaluated: RUNX2 and BSP. Immunohistochemical analyses were performed for detection of RUNX2, OPN, OCN, OPG, and RANKL antibodies and bone matrix proteins. Finally, four parameters were chosen for micro-CT analysis: trabecular number, separation and thickness, and connectivity density. Results Descriptive analysis showed similar findings among the experimental groups. Moreover, porous-hydrophilic surfaces presented a higher expression of RUNX2, which is probably an indicative of better osteogenesis; although the data from this study may be considered an insufficient support for a concrete statement. Conclusion Porous hydrophilic surface can improve and accelerate protein expression and bone formation. |
| first_indexed | 2024-12-19T13:32:25Z |
| format | Article |
| id | doaj.art-4ebd35c431cc4b58873f77413c18525c |
| institution | Directory Open Access Journal |
| issn | 2198-4034 |
| language | English |
| last_indexed | 2024-12-19T13:32:25Z |
| publishDate | 2020-12-01 |
| publisher | SpringerOpen |
| record_format | Article |
| series | International Journal of Implant Dentistry |
| spelling | doaj.art-4ebd35c431cc4b58873f77413c18525c2022-12-21T20:19:19ZengSpringerOpenInternational Journal of Implant Dentistry2198-40342020-12-016111410.1186/s40729-020-00279-7Gene expression, immunohistochemical and microarchitectural evaluation of bone formation around two implant surfaces placed in bone defects filled or not with bone substitute materialGuilherme dos Santos Trento0Jaqueline Suemi Hassumi1Paula Buzo Frigério2Ana Paula Farnezi Bassi3Roberta Okamoto4Marisa Aparecida Cabrini Gabrielli5Valfrido Antonio Pereira-Filho6Department of Diagnosis and Surgery, School of Dentistry, Sao Paulo State University (Unesp)Department of Oral and Maxillofacial Surgery, School of Dentistry, São Paulo State University (Unesp)Department of Oral and Maxillofacial Surgery, School of Dentistry, São Paulo State University (Unesp)Department of Oral and Maxillofacial Surgery, School of Dentistry, São Paulo State University (Unesp)Department of Oral and Maxillofacial Surgery, School of Dentistry, São Paulo State University (Unesp)Department of Diagnosis and Surgery, School of Dentistry, Sao Paulo State University (Unesp)Department of Diagnosis and Surgery, School of Dentistry, Sao Paulo State University (Unesp)Abstract Objective The aim of this study is to evaluate through gene expression, immunohistochemical and microtomographic (micro-CT) analysis the response of peri-implant bone tissue around titanium implants with different surface treatments, placed in bone defects filled or not with bone substitute materials. In addition, to investigate the hypothesis that porous-hydrophilic surface induces a faster bone formation. Materials and methods Twenty-six animals were divided into two groups according to implant surface treatment. In each tibia, a bone defect was created followed by the placement of one implant. On the left tibia, the defect was filled with blood clot (BC), and on the right tibia, the defect was filled with biphasic hydroxyapatite/β-tricalcium-phosphate (HA/TCP) generating four subgroups: BC-N: bone defect filled with blood clot and porous surface titanium implant installed; BC-A: bone defect filled with blood clot and porous-hydrophilic surface titanium implant installed; HA/TCP-N: bone defect filled with bone substitute material and porous surface titanium implant installed; and HA/TCP-A: bone defect filled with bone substitute material and porous-hydrophilic surface titanium implant installed. The animals were submitted to euthanasia at 15, 30, and 60 days after implant installation. The expression of two genes was evaluated: RUNX2 and BSP. Immunohistochemical analyses were performed for detection of RUNX2, OPN, OCN, OPG, and RANKL antibodies and bone matrix proteins. Finally, four parameters were chosen for micro-CT analysis: trabecular number, separation and thickness, and connectivity density. Results Descriptive analysis showed similar findings among the experimental groups. Moreover, porous-hydrophilic surfaces presented a higher expression of RUNX2, which is probably an indicative of better osteogenesis; although the data from this study may be considered an insufficient support for a concrete statement. Conclusion Porous hydrophilic surface can improve and accelerate protein expression and bone formation.https://doi.org/10.1186/s40729-020-00279-7Osseointegrated dental implantationBone graftingCell viability |
| spellingShingle | Guilherme dos Santos Trento Jaqueline Suemi Hassumi Paula Buzo Frigério Ana Paula Farnezi Bassi Roberta Okamoto Marisa Aparecida Cabrini Gabrielli Valfrido Antonio Pereira-Filho Gene expression, immunohistochemical and microarchitectural evaluation of bone formation around two implant surfaces placed in bone defects filled or not with bone substitute material International Journal of Implant Dentistry Osseointegrated dental implantation Bone grafting Cell viability |
| title | Gene expression, immunohistochemical and microarchitectural evaluation of bone formation around two implant surfaces placed in bone defects filled or not with bone substitute material |
| title_full | Gene expression, immunohistochemical and microarchitectural evaluation of bone formation around two implant surfaces placed in bone defects filled or not with bone substitute material |
| title_fullStr | Gene expression, immunohistochemical and microarchitectural evaluation of bone formation around two implant surfaces placed in bone defects filled or not with bone substitute material |
| title_full_unstemmed | Gene expression, immunohistochemical and microarchitectural evaluation of bone formation around two implant surfaces placed in bone defects filled or not with bone substitute material |
| title_short | Gene expression, immunohistochemical and microarchitectural evaluation of bone formation around two implant surfaces placed in bone defects filled or not with bone substitute material |
| title_sort | gene expression immunohistochemical and microarchitectural evaluation of bone formation around two implant surfaces placed in bone defects filled or not with bone substitute material |
| topic | Osseointegrated dental implantation Bone grafting Cell viability |
| url | https://doi.org/10.1186/s40729-020-00279-7 |
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