Polycaprolactone/gelatin electrospun nanofibres containing biologically produced tellurium nanoparticles as a potential wound dressing scaffold: Physicochemical, mechanical, and biological characterisation
Abstract The biologically synthesised tellurium nanoparticles (Te NPs) were applied in the fabrication of Te NP‐embedded polycaprolactone/gelatin (PCL/GEL) electrospun nanofibres and their antioxidant and in vivo wound healing properties were determined. The as‐synthesised nanofibres were characteri...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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Hindawi-IET
2021-05-01
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| Series: | IET Nanobiotechnology |
| Online Access: | https://doi.org/10.1049/nbt2.12020 |
| _version_ | 1797428863291097088 |
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| author | Mohsen Doostmohammadi Hamid Forootanfar Mojtaba Shakibaie Masoud Torkzadeh‐Mahani Hamid‐Reza Rahimi Elham Jafari Atefeh Ameri Alieh Ameri |
| author_facet | Mohsen Doostmohammadi Hamid Forootanfar Mojtaba Shakibaie Masoud Torkzadeh‐Mahani Hamid‐Reza Rahimi Elham Jafari Atefeh Ameri Alieh Ameri |
| author_sort | Mohsen Doostmohammadi |
| collection | DOAJ |
| description | Abstract The biologically synthesised tellurium nanoparticles (Te NPs) were applied in the fabrication of Te NP‐embedded polycaprolactone/gelatin (PCL/GEL) electrospun nanofibres and their antioxidant and in vivo wound healing properties were determined. The as‐synthesised nanofibres were characterised using scanning electron microscopy (SEM), energy‐dispersive X‐ray (EDX) spectroscopy and elemental mapping, thermogravimetric analysis (TGA), and Fourier‐transform infrared (FTIR) spectroscopy. The mechanical properties and surface hydrophobicity of scaffolds were investigated using tensile analysis and contact angle tests, respectively. The biocompatibility of the produced scaffolds on mouse embryonic fibroblast cells (3T3) was evaluated using MTT assay. The highest wound healing activity (score 15/19) was achieved for scaffolds containing Te NPs. The wounds treated with PCL/GEL/Te NPs had inflammation state equal to the positive control. Also, the mentioned scaffold represented positive effects on collagen formation and collagen fibre's horizontalisation in a dose‐dependent manner. The antioxidative potency of Te NP‐containing scaffolds was demonstrated with lower levels of malondialdehyde (MDA) and catalase (∼3 times) and a higher level of glutathione (GSH) (∼2 times) in PCL/GEL/Te NP‐treated samples than the negative control. The obtained results strongly demonstrated the healing activity of the produced nanofibres, and it can be inferred that scaffolds containing Te NPs are suitable for wound dressing. |
| first_indexed | 2024-03-09T09:04:58Z |
| format | Article |
| id | doaj.art-4ebf1a59c4264ecd9a89669b55d2ec72 |
| institution | Directory Open Access Journal |
| issn | 1751-8741 1751-875X |
| language | English |
| last_indexed | 2024-03-09T09:04:58Z |
| publishDate | 2021-05-01 |
| publisher | Hindawi-IET |
| record_format | Article |
| series | IET Nanobiotechnology |
| spelling | doaj.art-4ebf1a59c4264ecd9a89669b55d2ec722023-12-02T10:39:49ZengHindawi-IETIET Nanobiotechnology1751-87411751-875X2021-05-0115327729010.1049/nbt2.12020Polycaprolactone/gelatin electrospun nanofibres containing biologically produced tellurium nanoparticles as a potential wound dressing scaffold: Physicochemical, mechanical, and biological characterisationMohsen Doostmohammadi0Hamid Forootanfar1Mojtaba Shakibaie2Masoud Torkzadeh‐Mahani3Hamid‐Reza Rahimi4Elham Jafari5Atefeh Ameri6Alieh Ameri7Pharmaceutics Research Center Institute of Neuropharmacology Kerman University of Medical Sciences Kerman IranPharmaceutics Research Center Institute of Neuropharmacology Kerman University of Medical Sciences Kerman IranDepartment of Pharmaceutical Biotechnology Faculty of Pharmacy Kerman University of Medical Sciences Kerman IranDepartment of Biotechnology Institute of Science High Technology and Environmental Sciences Graduate University of Advanced Technology Kerman IranDepartment of Pharmacology and Toxicology Faculty of Pharmacy Kerman University of Medical Sciences Kerman IranPathology and Stem Cells Research Center Kerman University of Medical Science Kerman IranPharmaceutical Sciences and Cosmetic Products Research Center Kerman University of Medical Sciences Kerman IranDepartment of Medicinal Chemistry Faculty of Pharmacy Kerman University of Medical Sciences Kerman IranAbstract The biologically synthesised tellurium nanoparticles (Te NPs) were applied in the fabrication of Te NP‐embedded polycaprolactone/gelatin (PCL/GEL) electrospun nanofibres and their antioxidant and in vivo wound healing properties were determined. The as‐synthesised nanofibres were characterised using scanning electron microscopy (SEM), energy‐dispersive X‐ray (EDX) spectroscopy and elemental mapping, thermogravimetric analysis (TGA), and Fourier‐transform infrared (FTIR) spectroscopy. The mechanical properties and surface hydrophobicity of scaffolds were investigated using tensile analysis and contact angle tests, respectively. The biocompatibility of the produced scaffolds on mouse embryonic fibroblast cells (3T3) was evaluated using MTT assay. The highest wound healing activity (score 15/19) was achieved for scaffolds containing Te NPs. The wounds treated with PCL/GEL/Te NPs had inflammation state equal to the positive control. Also, the mentioned scaffold represented positive effects on collagen formation and collagen fibre's horizontalisation in a dose‐dependent manner. The antioxidative potency of Te NP‐containing scaffolds was demonstrated with lower levels of malondialdehyde (MDA) and catalase (∼3 times) and a higher level of glutathione (GSH) (∼2 times) in PCL/GEL/Te NP‐treated samples than the negative control. The obtained results strongly demonstrated the healing activity of the produced nanofibres, and it can be inferred that scaffolds containing Te NPs are suitable for wound dressing.https://doi.org/10.1049/nbt2.12020 |
| spellingShingle | Mohsen Doostmohammadi Hamid Forootanfar Mojtaba Shakibaie Masoud Torkzadeh‐Mahani Hamid‐Reza Rahimi Elham Jafari Atefeh Ameri Alieh Ameri Polycaprolactone/gelatin electrospun nanofibres containing biologically produced tellurium nanoparticles as a potential wound dressing scaffold: Physicochemical, mechanical, and biological characterisation IET Nanobiotechnology |
| title | Polycaprolactone/gelatin electrospun nanofibres containing biologically produced tellurium nanoparticles as a potential wound dressing scaffold: Physicochemical, mechanical, and biological characterisation |
| title_full | Polycaprolactone/gelatin electrospun nanofibres containing biologically produced tellurium nanoparticles as a potential wound dressing scaffold: Physicochemical, mechanical, and biological characterisation |
| title_fullStr | Polycaprolactone/gelatin electrospun nanofibres containing biologically produced tellurium nanoparticles as a potential wound dressing scaffold: Physicochemical, mechanical, and biological characterisation |
| title_full_unstemmed | Polycaprolactone/gelatin electrospun nanofibres containing biologically produced tellurium nanoparticles as a potential wound dressing scaffold: Physicochemical, mechanical, and biological characterisation |
| title_short | Polycaprolactone/gelatin electrospun nanofibres containing biologically produced tellurium nanoparticles as a potential wound dressing scaffold: Physicochemical, mechanical, and biological characterisation |
| title_sort | polycaprolactone gelatin electrospun nanofibres containing biologically produced tellurium nanoparticles as a potential wound dressing scaffold physicochemical mechanical and biological characterisation |
| url | https://doi.org/10.1049/nbt2.12020 |
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