Genome‐wide analysis of PvMADS in common bean and functional characterization of PvMADS31 in Arabidopsis thaliana as a player in abiotic stress responses
Abstract Changing climatic conditions with rising temperatures and altered precipitation patterns pose significant challenges to agricultural productivity, particularly for common bean crops. Transcription factors (TFs) are crucial regulators that can mitigate the impact of biotic and abiotic stress...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2024-03-01
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| Series: | The Plant Genome |
| Online Access: | https://doi.org/10.1002/tpg2.20432 |
| _version_ | 1827312212941733888 |
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| author | Karam Mostafa Bayram Ali Yerlikaya Mohamed Farah Abdulla Abdullah Aydin Seher Yerlikaya Musa Kavas |
| author_facet | Karam Mostafa Bayram Ali Yerlikaya Mohamed Farah Abdulla Abdullah Aydin Seher Yerlikaya Musa Kavas |
| author_sort | Karam Mostafa |
| collection | DOAJ |
| description | Abstract Changing climatic conditions with rising temperatures and altered precipitation patterns pose significant challenges to agricultural productivity, particularly for common bean crops. Transcription factors (TFs) are crucial regulators that can mitigate the impact of biotic and abiotic stresses on crop production. The MADS‐box TFs family has been implicated in various plant physiological processes, including stress‐responsive mechanisms. However, their role in common bean and their response to stressful conditions remain poorly understood. Here, we identified 35 MADS‐box gene family members in common bean, with conserved MADS‐box domains and other functional domains. Gene duplication events were observed, suggesting the significance of duplication in the evolutionary development of gene families. The analysis of promoter regions revealed diverse elements, including stress‐responsive elements, indicating their potential involvement in stress responses. Notably, PvMADS31, a member of the PvMADS‐box gene family, demonstrated rapid upregulation under various abiotic stress conditions, including NaCl, polyethylene glycol, drought, and abscisic acid (ABA) treatments. Transgenic plants overexpressing PvMADS31 displayed enhanced lateral root development, root elongation, and seed germination under stress conditions. Furthermore, PvMADS31 overexpression in Arabidopsis resulted in improved drought tolerance, likely attributed to the enhanced scavenging of ROS and increased proline accumulation. These findings suggest that PvMADS31 might play a crucial role in modulating seed germination, root development, and stress responses, potentially through its involvement in auxin and ABA signaling pathways. Overall, this study provides valuable insights into the potential roles of PvMADS‐box genes in abiotic stress responses in common bean, offering prospects for crop improvement strategies to enhance resilience under changing environmental conditions. |
| first_indexed | 2024-04-24T21:39:18Z |
| format | Article |
| id | doaj.art-4ec33f61312c4041b0df9c4679b1f63e |
| institution | Directory Open Access Journal |
| issn | 1940-3372 |
| language | English |
| last_indexed | 2024-04-24T21:39:18Z |
| publishDate | 2024-03-01 |
| publisher | Wiley |
| record_format | Article |
| series | The Plant Genome |
| spelling | doaj.art-4ec33f61312c4041b0df9c4679b1f63e2024-03-21T11:34:19ZengWileyThe Plant Genome1940-33722024-03-01171n/an/a10.1002/tpg2.20432Genome‐wide analysis of PvMADS in common bean and functional characterization of PvMADS31 in Arabidopsis thaliana as a player in abiotic stress responsesKaram Mostafa0Bayram Ali Yerlikaya1Mohamed Farah Abdulla2Abdullah Aydin3Seher Yerlikaya4Musa Kavas5Department of Agricultural Biotechnology, Faculty of Agriculture Ondokuz Mayis University Samsun TurkeyDepartment of Agricultural Biotechnology, Faculty of Agriculture Ondokuz Mayis University Samsun TurkeyDepartment of Agricultural Biotechnology, Faculty of Agriculture Ondokuz Mayis University Samsun TurkeyDepartment of Agricultural Biotechnology, Faculty of Agriculture Ondokuz Mayis University Samsun TurkeyDepartment of Agricultural Biotechnology, Faculty of Agriculture Ondokuz Mayis University Samsun TurkeyDepartment of Agricultural Biotechnology, Faculty of Agriculture Ondokuz Mayis University Samsun TurkeyAbstract Changing climatic conditions with rising temperatures and altered precipitation patterns pose significant challenges to agricultural productivity, particularly for common bean crops. Transcription factors (TFs) are crucial regulators that can mitigate the impact of biotic and abiotic stresses on crop production. The MADS‐box TFs family has been implicated in various plant physiological processes, including stress‐responsive mechanisms. However, their role in common bean and their response to stressful conditions remain poorly understood. Here, we identified 35 MADS‐box gene family members in common bean, with conserved MADS‐box domains and other functional domains. Gene duplication events were observed, suggesting the significance of duplication in the evolutionary development of gene families. The analysis of promoter regions revealed diverse elements, including stress‐responsive elements, indicating their potential involvement in stress responses. Notably, PvMADS31, a member of the PvMADS‐box gene family, demonstrated rapid upregulation under various abiotic stress conditions, including NaCl, polyethylene glycol, drought, and abscisic acid (ABA) treatments. Transgenic plants overexpressing PvMADS31 displayed enhanced lateral root development, root elongation, and seed germination under stress conditions. Furthermore, PvMADS31 overexpression in Arabidopsis resulted in improved drought tolerance, likely attributed to the enhanced scavenging of ROS and increased proline accumulation. These findings suggest that PvMADS31 might play a crucial role in modulating seed germination, root development, and stress responses, potentially through its involvement in auxin and ABA signaling pathways. Overall, this study provides valuable insights into the potential roles of PvMADS‐box genes in abiotic stress responses in common bean, offering prospects for crop improvement strategies to enhance resilience under changing environmental conditions.https://doi.org/10.1002/tpg2.20432 |
| spellingShingle | Karam Mostafa Bayram Ali Yerlikaya Mohamed Farah Abdulla Abdullah Aydin Seher Yerlikaya Musa Kavas Genome‐wide analysis of PvMADS in common bean and functional characterization of PvMADS31 in Arabidopsis thaliana as a player in abiotic stress responses The Plant Genome |
| title | Genome‐wide analysis of PvMADS in common bean and functional characterization of PvMADS31 in Arabidopsis thaliana as a player in abiotic stress responses |
| title_full | Genome‐wide analysis of PvMADS in common bean and functional characterization of PvMADS31 in Arabidopsis thaliana as a player in abiotic stress responses |
| title_fullStr | Genome‐wide analysis of PvMADS in common bean and functional characterization of PvMADS31 in Arabidopsis thaliana as a player in abiotic stress responses |
| title_full_unstemmed | Genome‐wide analysis of PvMADS in common bean and functional characterization of PvMADS31 in Arabidopsis thaliana as a player in abiotic stress responses |
| title_short | Genome‐wide analysis of PvMADS in common bean and functional characterization of PvMADS31 in Arabidopsis thaliana as a player in abiotic stress responses |
| title_sort | genome wide analysis of pvmads in common bean and functional characterization of pvmads31 in arabidopsis thaliana as a player in abiotic stress responses |
| url | https://doi.org/10.1002/tpg2.20432 |
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