Multiparametric flow cytometry using near-infrared fluorescent proteins engineered from bacterial phytochromes.

Engineering of fluorescent proteins (FPs) has followed a trend of achieving longer fluorescence wavelengths, with the ultimate goal of producing proteins with both excitation and emission in the near-infrared (NIR) region of the spectrum. Flow cytometers are now almost universally equipped with red...

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Bibliographic Details
Main Authors: William G Telford, Daria M Shcherbakova, David Buschke, Teresa S Hawley, Vladislav V Verkhusha
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2015-01-01
Series:PLoS ONE
Online Access:http://europepmc.org/articles/PMC4374955?pdf=render

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