Seroepidemiological study of human cysticercosis with blood samples collected on filter paper, in Lages, State of Santa Catarina, Brazil, 2004-2005
INTRODUCTION: Human serofrequency of antibodies against Taenia solium antigens was determined and risk factors for cysticercosis transmission were identified. METHODS: Individuals (n=878) from periurban and rural locations of Lages, SC, were interviewed to gather demographic, sanitary and health inf...
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Sociedade Brasileira de Medicina Tropical (SBMT)
2011-06-01
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| Series: | Revista da Sociedade Brasileira de Medicina Tropical |
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| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822011000300016&lng=en&tlng=en |
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| author | Maria Márcia Imenes Ishida Marília Sirianni dos Santos Almeida Noeli Maria Espíndola Alberto Iha Diana Ana Pereira Jean Gabriel de Souza Theopi Rados Varvakis Adelaide José Vaz |
| author_facet | Maria Márcia Imenes Ishida Marília Sirianni dos Santos Almeida Noeli Maria Espíndola Alberto Iha Diana Ana Pereira Jean Gabriel de Souza Theopi Rados Varvakis Adelaide José Vaz |
| author_sort | Maria Márcia Imenes Ishida |
| collection | DOAJ |
| description | INTRODUCTION: Human serofrequency of antibodies against Taenia solium antigens was determined and risk factors for cysticercosis transmission were identified. METHODS: Individuals (n=878) from periurban and rural locations of Lages, SC, were interviewed to gather demographic, sanitary and health information. Interviews and blood sample collections by finger prick on Whatman filter paper were performed from August 2004 to May 2005. Observation determined that 850 samples were suitable for analysis and were tested by ELISA using vesicular fluid of Taenia crassiceps heterologous antigen. To ensure the reliability of the results, 77 samples of the dried blood were matched with sera. The reactive samples were submitted to a serum confirmatory immunoblot (IB) test using purified Taenia crassiceps glycoproteins. RESULTS: The ELISA results for the dried blood and serum samples were statistically consistent. ELISA was positive in 186 (21.9%) out of 850 individuals. A group of 213 individuals were asked to collect vein blood for IB (186 with positive result in ELISA and 27 with inappropriate whole blood samples) and 130 attended the request. The IB was positive in 29 (3.4%) out of 850 individuals. A significant correlation (p = 0.0364) was determined among individuals who tested positive in the IB assay who practiced both pig rearing and kitchen gardening. CONCLUSIONS: ELISA with dried blood eluted from filter paper was suitable for cysticercosis population surveys. In Lages, human infection was associated with pig rearing and kitchen gardening. The prevalence index was compatible with other Latin American endemic areas. |
| first_indexed | 2024-12-12T14:14:06Z |
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| institution | Directory Open Access Journal |
| issn | 1678-9849 |
| language | English |
| last_indexed | 2024-12-12T14:14:06Z |
| publishDate | 2011-06-01 |
| publisher | Sociedade Brasileira de Medicina Tropical (SBMT) |
| record_format | Article |
| series | Revista da Sociedade Brasileira de Medicina Tropical |
| spelling | doaj.art-4ec9c4d64a9a4910a159d50b94ee681b2022-12-22T00:21:57ZengSociedade Brasileira de Medicina Tropical (SBMT)Revista da Sociedade Brasileira de Medicina Tropical1678-98492011-06-0144333934310.1590/s0037-86822011005000040S0037-86822011000300016Seroepidemiological study of human cysticercosis with blood samples collected on filter paper, in Lages, State of Santa Catarina, Brazil, 2004-2005Maria Márcia Imenes Ishida0Marília Sirianni dos Santos Almeida1Noeli Maria Espíndola2Alberto Iha3Diana Ana Pereira4Jean Gabriel de Souza5Theopi Rados Varvakis6Adelaide José Vaz7Universidade Federal de Santa CatarinaFundação Oswaldo CruzUniversidade de São PauloUniversidade de São PauloUniversidade Federal de Santa CatarinaUniversidade Federal de Santa CatarinaUniversidade Federal de Santa CatarinaUniversidade de São PauloINTRODUCTION: Human serofrequency of antibodies against Taenia solium antigens was determined and risk factors for cysticercosis transmission were identified. METHODS: Individuals (n=878) from periurban and rural locations of Lages, SC, were interviewed to gather demographic, sanitary and health information. Interviews and blood sample collections by finger prick on Whatman filter paper were performed from August 2004 to May 2005. Observation determined that 850 samples were suitable for analysis and were tested by ELISA using vesicular fluid of Taenia crassiceps heterologous antigen. To ensure the reliability of the results, 77 samples of the dried blood were matched with sera. The reactive samples were submitted to a serum confirmatory immunoblot (IB) test using purified Taenia crassiceps glycoproteins. RESULTS: The ELISA results for the dried blood and serum samples were statistically consistent. ELISA was positive in 186 (21.9%) out of 850 individuals. A group of 213 individuals were asked to collect vein blood for IB (186 with positive result in ELISA and 27 with inappropriate whole blood samples) and 130 attended the request. The IB was positive in 29 (3.4%) out of 850 individuals. A significant correlation (p = 0.0364) was determined among individuals who tested positive in the IB assay who practiced both pig rearing and kitchen gardening. CONCLUSIONS: ELISA with dried blood eluted from filter paper was suitable for cysticercosis population surveys. In Lages, human infection was associated with pig rearing and kitchen gardening. The prevalence index was compatible with other Latin American endemic areas.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822011000300016&lng=en&tlng=enCisticercoseTaenia crassicepsImunodiagnósticoEpidemiologia |
| spellingShingle | Maria Márcia Imenes Ishida Marília Sirianni dos Santos Almeida Noeli Maria Espíndola Alberto Iha Diana Ana Pereira Jean Gabriel de Souza Theopi Rados Varvakis Adelaide José Vaz Seroepidemiological study of human cysticercosis with blood samples collected on filter paper, in Lages, State of Santa Catarina, Brazil, 2004-2005 Revista da Sociedade Brasileira de Medicina Tropical Cisticercose Taenia crassiceps Imunodiagnóstico Epidemiologia |
| title | Seroepidemiological study of human cysticercosis with blood samples collected on filter paper, in Lages, State of Santa Catarina, Brazil, 2004-2005 |
| title_full | Seroepidemiological study of human cysticercosis with blood samples collected on filter paper, in Lages, State of Santa Catarina, Brazil, 2004-2005 |
| title_fullStr | Seroepidemiological study of human cysticercosis with blood samples collected on filter paper, in Lages, State of Santa Catarina, Brazil, 2004-2005 |
| title_full_unstemmed | Seroepidemiological study of human cysticercosis with blood samples collected on filter paper, in Lages, State of Santa Catarina, Brazil, 2004-2005 |
| title_short | Seroepidemiological study of human cysticercosis with blood samples collected on filter paper, in Lages, State of Santa Catarina, Brazil, 2004-2005 |
| title_sort | seroepidemiological study of human cysticercosis with blood samples collected on filter paper in lages state of santa catarina brazil 2004 2005 |
| topic | Cisticercose Taenia crassiceps Imunodiagnóstico Epidemiologia |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822011000300016&lng=en&tlng=en |
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