MOLECULAR IDENTIFICATION OF ISOLATES <i>ANAPLASMA MARGINALE</i> FOUND IN BLOOD OF CATTLE ON THE TERRITORY OF MOSCOW REGION
Objective of research: To detect and identify by molecular and genetic methods isolates Anaplasma marginale circulating on the territory of Moscow region. Materials and methods: Blood samples were taken from cattle on the territory of Moscow region in 2015. DNA was isolated from whole blood by the k...
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Federal Scientific Centre VIEV
2019-10-01
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| Series: | Российский паразитологический журнал |
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| Online Access: | https://vniigis.elpub.ru/jour/article/view/405 |
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| author | F. I. Vasilevich S. N. Kovalchuk A. V. Babiy A. V. Arkhipov A. L. Arkhipova T. T. Glazko G. Yu. Kosovskiy |
| author_facet | F. I. Vasilevich S. N. Kovalchuk A. V. Babiy A. V. Arkhipov A. L. Arkhipova T. T. Glazko G. Yu. Kosovskiy |
| author_sort | F. I. Vasilevich |
| collection | DOAJ |
| description | Objective of research: To detect and identify by molecular and genetic methods isolates Anaplasma marginale circulating on the territory of Moscow region. Materials and methods: Blood samples were taken from cattle on the territory of Moscow region in 2015. DNA was isolated from whole blood by the kit «Sorb-M». Identification of animals infected with A. marginale and estimation of the parasitemia level were carried out by real-time PCR methods. Typing of A. marginale strains was performed by modifying the primer structure. The received gene fragments were cloned in Escherichia сoli cells. Targeted selection of recombinant clones of E. coli was conducted by PCR methods using standard primers M13 with the following analysis of reaction products by 1,5% agarose gel electrophoresis. Results and discussion: It was shown that seven of thirty animals were carriers of A. marginale strains; levels of parasitemia range from 3,6 × 103 to 5,4 × 105 per 1 ml blood. The analysis of msp4 gene sequence of A. marginale isolates shows their belonging to two already known genotypes. The results obtained can be used for epidemiological monitoring of anaplasmosis on the territory of Moscow region as well as taken into account for development of vaccines. |
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| id | doaj.art-4ecc0d84abd54b83b94bf76abff009ca |
| institution | Directory Open Access Journal |
| issn | 1998-8435 2541-7843 |
| language | Russian |
| last_indexed | 2025-03-14T11:50:24Z |
| publishDate | 2019-10-01 |
| publisher | Federal Scientific Centre VIEV |
| record_format | Article |
| series | Российский паразитологический журнал |
| spelling | doaj.art-4ecc0d84abd54b83b94bf76abff009ca2025-03-02T09:49:51ZrusFederal Scientific Centre VIEVРоссийский паразитологический журнал1998-84352541-78432019-10-01402179182402MOLECULAR IDENTIFICATION OF ISOLATES <i>ANAPLASMA MARGINALE</i> FOUND IN BLOOD OF CATTLE ON THE TERRITORY OF MOSCOW REGIONF. I. Vasilevich0S. N. Kovalchuk1A. V. Babiy2A. V. Arkhipov3A. L. Arkhipova4T. T. Glazko5G. Yu. Kosovskiy6Moscow State Academy of Veterinary Medicine and Biotechnology named after K.I. SkryabinCenter of Experimental Embryology and Reproductive BiotechnologiesCenter of Experimental Embryology and Reproductive BiotechnologiesCenter of Experimental Embryology and Reproductive BiotechnologiesCenter of Experimental Embryology and Reproductive BiotechnologiesCenter of Experimental Embryology and Reproductive BiotechnologiesCenter of Experimental Embryology and Reproductive BiotechnologiesObjective of research: To detect and identify by molecular and genetic methods isolates Anaplasma marginale circulating on the territory of Moscow region. Materials and methods: Blood samples were taken from cattle on the territory of Moscow region in 2015. DNA was isolated from whole blood by the kit «Sorb-M». Identification of animals infected with A. marginale and estimation of the parasitemia level were carried out by real-time PCR methods. Typing of A. marginale strains was performed by modifying the primer structure. The received gene fragments were cloned in Escherichia сoli cells. Targeted selection of recombinant clones of E. coli was conducted by PCR methods using standard primers M13 with the following analysis of reaction products by 1,5% agarose gel electrophoresis. Results and discussion: It was shown that seven of thirty animals were carriers of A. marginale strains; levels of parasitemia range from 3,6 × 103 to 5,4 × 105 per 1 ml blood. The analysis of msp4 gene sequence of A. marginale isolates shows their belonging to two already known genotypes. The results obtained can be used for epidemiological monitoring of anaplasmosis on the territory of Moscow region as well as taken into account for development of vaccines.https://vniigis.elpub.ru/jour/article/view/405анаплазмозanaplasma marginaleкрупный рогатый скотпцрпаразитемиягенотипированиеanaplasmosisanaplasma marginalecattlepcrparasitemiagenotyping |
| spellingShingle | F. I. Vasilevich S. N. Kovalchuk A. V. Babiy A. V. Arkhipov A. L. Arkhipova T. T. Glazko G. Yu. Kosovskiy MOLECULAR IDENTIFICATION OF ISOLATES <i>ANAPLASMA MARGINALE</i> FOUND IN BLOOD OF CATTLE ON THE TERRITORY OF MOSCOW REGION Российский паразитологический журнал анаплазмоз anaplasma marginale крупный рогатый скот пцр паразитемия генотипирование anaplasmosis anaplasma marginale cattle pcr parasitemia genotyping |
| title | MOLECULAR IDENTIFICATION OF ISOLATES <i>ANAPLASMA MARGINALE</i> FOUND IN BLOOD OF CATTLE ON THE TERRITORY OF MOSCOW REGION |
| title_full | MOLECULAR IDENTIFICATION OF ISOLATES <i>ANAPLASMA MARGINALE</i> FOUND IN BLOOD OF CATTLE ON THE TERRITORY OF MOSCOW REGION |
| title_fullStr | MOLECULAR IDENTIFICATION OF ISOLATES <i>ANAPLASMA MARGINALE</i> FOUND IN BLOOD OF CATTLE ON THE TERRITORY OF MOSCOW REGION |
| title_full_unstemmed | MOLECULAR IDENTIFICATION OF ISOLATES <i>ANAPLASMA MARGINALE</i> FOUND IN BLOOD OF CATTLE ON THE TERRITORY OF MOSCOW REGION |
| title_short | MOLECULAR IDENTIFICATION OF ISOLATES <i>ANAPLASMA MARGINALE</i> FOUND IN BLOOD OF CATTLE ON THE TERRITORY OF MOSCOW REGION |
| title_sort | molecular identification of isolates i anaplasma marginale i found in blood of cattle on the territory of moscow region |
| topic | анаплазмоз anaplasma marginale крупный рогатый скот пцр паразитемия генотипирование anaplasmosis anaplasma marginale cattle pcr parasitemia genotyping |
| url | https://vniigis.elpub.ru/jour/article/view/405 |
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