A polymorphism in porcine miR-22 is associated with pork color
MicroRNAs (miRNAs) are posttranscriptional regulators that play key roles in meat color regulation. Changes in miRNA expression affect their target mRNAs, leading to multifunctional effects on biological processes and phenotypes. In this study, a G > A mutation site located upstream of the pr...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2022-07-01
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| Series: | Frontiers in Veterinary Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fvets.2022.939440/full |
| _version_ | 1828430426820050944 |
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| author | Han Wang Zhonghao Shen Ruihua Huang Ayong Zhao Jiani Jiang Pinghua Li Xiaolong Zhou Songbai Yang Liming Hou |
| author_facet | Han Wang Zhonghao Shen Ruihua Huang Ayong Zhao Jiani Jiang Pinghua Li Xiaolong Zhou Songbai Yang Liming Hou |
| author_sort | Han Wang |
| collection | DOAJ |
| description | MicroRNAs (miRNAs) are posttranscriptional regulators that play key roles in meat color regulation. Changes in miRNA expression affect their target mRNAs, leading to multifunctional effects on biological processes and phenotypes. In this study, a G > A mutation site located upstream of the precursor miR-22 sequence in Suhuai pigs was significantly correlated with the meat color parameter a*(redness) of the porcine longissimus dorsi (LD) muscle. AA genotype individuals had the highest average meat color a* value and the lowest miR-22 level. When G > A mutation was performed in the miR-22 overexpression vector, miR-22 expression significantly decreased. Considering that Ca2+ homeostasis is closely related to pig meat color, our results further demonstrated that ELOVL6 is a direct target of miR-22 in pigs. The effects of miR-22 on skeletal muscle intracellular Ca2+ were partially caused by the suppression of ELOVL6 expression. |
| first_indexed | 2024-12-10T17:48:24Z |
| format | Article |
| id | doaj.art-4ecc7a61875f4cbea66b687b0543c595 |
| institution | Directory Open Access Journal |
| issn | 2297-1769 |
| language | English |
| last_indexed | 2024-12-10T17:48:24Z |
| publishDate | 2022-07-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Veterinary Science |
| spelling | doaj.art-4ecc7a61875f4cbea66b687b0543c5952022-12-22T01:39:08ZengFrontiers Media S.A.Frontiers in Veterinary Science2297-17692022-07-01910.3389/fvets.2022.939440939440A polymorphism in porcine miR-22 is associated with pork colorHan Wang0Zhonghao Shen1Ruihua Huang2Ayong Zhao3Jiani Jiang4Pinghua Li5Xiaolong Zhou6Songbai Yang7Liming Hou8Key Laboratory of Applied Technology on Green-Eco-Healthy Animal Husbandry of Zhejiang Province, Department of Animal Breeding, College of Animal Science and Technology, College of Veterinary Medicine, Zhejiang A&F University, Hangzhou, ChinaKey Laboratory of Applied Technology on Green-Eco-Healthy Animal Husbandry of Zhejiang Province, Department of Animal Breeding, College of Animal Science and Technology, College of Veterinary Medicine, Zhejiang A&F University, Hangzhou, ChinaInstitute of Swine Science, Department of Animal Breeding, College of Animal Science and Technology, Nanjing Agricultural University, Nanjing, ChinaKey Laboratory of Applied Technology on Green-Eco-Healthy Animal Husbandry of Zhejiang Province, Department of Animal Breeding, College of Animal Science and Technology, College of Veterinary Medicine, Zhejiang A&F University, Hangzhou, ChinaDepartment of Statistical Sciences, University of Toronto, Toronto, ON, CanadaInstitute of Swine Science, Department of Animal Breeding, College of Animal Science and Technology, Nanjing Agricultural University, Nanjing, ChinaKey Laboratory of Applied Technology on Green-Eco-Healthy Animal Husbandry of Zhejiang Province, Department of Animal Breeding, College of Animal Science and Technology, College of Veterinary Medicine, Zhejiang A&F University, Hangzhou, ChinaKey Laboratory of Applied Technology on Green-Eco-Healthy Animal Husbandry of Zhejiang Province, Department of Animal Breeding, College of Animal Science and Technology, College of Veterinary Medicine, Zhejiang A&F University, Hangzhou, ChinaInstitute of Swine Science, Department of Animal Breeding, College of Animal Science and Technology, Nanjing Agricultural University, Nanjing, ChinaMicroRNAs (miRNAs) are posttranscriptional regulators that play key roles in meat color regulation. Changes in miRNA expression affect their target mRNAs, leading to multifunctional effects on biological processes and phenotypes. In this study, a G > A mutation site located upstream of the precursor miR-22 sequence in Suhuai pigs was significantly correlated with the meat color parameter a*(redness) of the porcine longissimus dorsi (LD) muscle. AA genotype individuals had the highest average meat color a* value and the lowest miR-22 level. When G > A mutation was performed in the miR-22 overexpression vector, miR-22 expression significantly decreased. Considering that Ca2+ homeostasis is closely related to pig meat color, our results further demonstrated that ELOVL6 is a direct target of miR-22 in pigs. The effects of miR-22 on skeletal muscle intracellular Ca2+ were partially caused by the suppression of ELOVL6 expression.https://www.frontiersin.org/articles/10.3389/fvets.2022.939440/fullpigmeat colormiR-22ELOVL6intracellular calcium concentrationmutation |
| spellingShingle | Han Wang Zhonghao Shen Ruihua Huang Ayong Zhao Jiani Jiang Pinghua Li Xiaolong Zhou Songbai Yang Liming Hou A polymorphism in porcine miR-22 is associated with pork color Frontiers in Veterinary Science pig meat color miR-22 ELOVL6 intracellular calcium concentration mutation |
| title | A polymorphism in porcine miR-22 is associated with pork color |
| title_full | A polymorphism in porcine miR-22 is associated with pork color |
| title_fullStr | A polymorphism in porcine miR-22 is associated with pork color |
| title_full_unstemmed | A polymorphism in porcine miR-22 is associated with pork color |
| title_short | A polymorphism in porcine miR-22 is associated with pork color |
| title_sort | polymorphism in porcine mir 22 is associated with pork color |
| topic | pig meat color miR-22 ELOVL6 intracellular calcium concentration mutation |
| url | https://www.frontiersin.org/articles/10.3389/fvets.2022.939440/full |
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