Effect of Different Dilution Methods and Ratios of Ram Semen on Sperm Parameters after Cryopreservation
The dilution method and ratio were tested to assess their effects on the <i>Hu</i> ram semen after cryopreservation. Experiment I aimed to explore the effect of various dilution ratios (1:1, 1:2, 1:3, 1:4) of diluent I (Tris-based and egg yolk) under the condition of 1:1 dilution of dilu...
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MDPI AG
2024-03-01
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| author | Liuming Zhang Xuyang Wang Caiyu Jiang Tariq Sohail Yuxuan Sun Xiaomei Sun Jian Wang Yongjun Li |
| author_facet | Liuming Zhang Xuyang Wang Caiyu Jiang Tariq Sohail Yuxuan Sun Xiaomei Sun Jian Wang Yongjun Li |
| author_sort | Liuming Zhang |
| collection | DOAJ |
| description | The dilution method and ratio were tested to assess their effects on the <i>Hu</i> ram semen after cryopreservation. Experiment I aimed to explore the effect of various dilution ratios (1:1, 1:2, 1:3, 1:4) of diluent I (Tris-based and egg yolk) under the condition of 1:1 dilution of diluent II (diluent I and glycerol) on the <i>Hu</i> ram semen preserved in liquid nitrogen regarding spermatozoa motility and kinetic parameters. Experiment II aimed to investigate the effect of various dilution ratios (1:1, 1:2, 1:3, 1:4) of diluent I under the condition of 1:2 dilution of diluent II to the <i>Hu</i> ram semen for cryopreservation on spermatozoa motility and kinetic parameters. The purpose of experiment III is to assess the effect of various dilution methods and ratios on the cryopreservation of <i>Hu</i> ram semen by detecting spermatozoa motility, kinetic parameters, plasma membrane integrity, acrosome integrity and reactive oxygen species (ROS) level. Experiment III includes four groups: one-step dilution method and two-step dilution method. The two-step dilution method includes two groups: 1:2, 1:1 and 1:3, 1:2, and the one-step dilution method includes two groups: 1:5 and 1:11. The results indicated that the post-thawed spermatozoa total motility (TM), progressive motility (PM) and average motion degree (MAD) were highest in the 1:2 group and significantly higher (<i>p</i> < 0.05) than those in the 1:1 and 1:4 groups under the condition of 1:1 dilution of diluent II. The post-thawed spermatozoa TM and PM of the 1:3 group were significantly higher (<i>p</i> < 0.05) than those of the other groups under the condition of 1:2 dilution of diluent II. The post-thawed spermatozoa TM, PM, plasma membrane integrity and acrosome integrity of the two-step group (1:3, 1:2) were the highest and significantly higher (<i>p</i> < 0.05) than those in the other groups. Additionally, the post-thawed spermatozoa ROS level of the two-step group (1:3, 1:2) was significantly lower (<i>p</i> < 0.05) than that in the one-step groups (1:5 and 1:11). Therefore, a two-step dilution (1:3, 1:2) was found to be the most suitable method and ratio for diluting the <i>Hu</i> ram semen after cryopreservation. |
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| spelling | doaj.art-4ecdfa8993f249af8fc24ab70335e6e52024-03-27T13:17:48ZengMDPI AGAnimals2076-26152024-03-0114690710.3390/ani14060907Effect of Different Dilution Methods and Ratios of Ram Semen on Sperm Parameters after CryopreservationLiuming Zhang0Xuyang Wang1Caiyu Jiang2Tariq Sohail3Yuxuan Sun4Xiaomei Sun5Jian Wang6Yongjun Li7Key Laboratory for Animal Genetics & Molecular Breeding of Jiangsu Province, College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, ChinaKey Laboratory for Animal Genetics & Molecular Breeding of Jiangsu Province, College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, ChinaKey Laboratory for Animal Genetics & Molecular Breeding of Jiangsu Province, College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, ChinaKey Laboratory for Animal Genetics & Molecular Breeding of Jiangsu Province, College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, ChinaKey Laboratory for Animal Genetics & Molecular Breeding of Jiangsu Province, College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, ChinaKey Laboratory for Animal Genetics & Molecular Breeding of Jiangsu Province, College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, ChinaKey Laboratory for Animal Genetics & Molecular Breeding of Jiangsu Province, College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, ChinaKey Laboratory for Animal Genetics & Molecular Breeding of Jiangsu Province, College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, ChinaThe dilution method and ratio were tested to assess their effects on the <i>Hu</i> ram semen after cryopreservation. Experiment I aimed to explore the effect of various dilution ratios (1:1, 1:2, 1:3, 1:4) of diluent I (Tris-based and egg yolk) under the condition of 1:1 dilution of diluent II (diluent I and glycerol) on the <i>Hu</i> ram semen preserved in liquid nitrogen regarding spermatozoa motility and kinetic parameters. Experiment II aimed to investigate the effect of various dilution ratios (1:1, 1:2, 1:3, 1:4) of diluent I under the condition of 1:2 dilution of diluent II to the <i>Hu</i> ram semen for cryopreservation on spermatozoa motility and kinetic parameters. The purpose of experiment III is to assess the effect of various dilution methods and ratios on the cryopreservation of <i>Hu</i> ram semen by detecting spermatozoa motility, kinetic parameters, plasma membrane integrity, acrosome integrity and reactive oxygen species (ROS) level. Experiment III includes four groups: one-step dilution method and two-step dilution method. The two-step dilution method includes two groups: 1:2, 1:1 and 1:3, 1:2, and the one-step dilution method includes two groups: 1:5 and 1:11. The results indicated that the post-thawed spermatozoa total motility (TM), progressive motility (PM) and average motion degree (MAD) were highest in the 1:2 group and significantly higher (<i>p</i> < 0.05) than those in the 1:1 and 1:4 groups under the condition of 1:1 dilution of diluent II. The post-thawed spermatozoa TM and PM of the 1:3 group were significantly higher (<i>p</i> < 0.05) than those of the other groups under the condition of 1:2 dilution of diluent II. The post-thawed spermatozoa TM, PM, plasma membrane integrity and acrosome integrity of the two-step group (1:3, 1:2) were the highest and significantly higher (<i>p</i> < 0.05) than those in the other groups. Additionally, the post-thawed spermatozoa ROS level of the two-step group (1:3, 1:2) was significantly lower (<i>p</i> < 0.05) than that in the one-step groups (1:5 and 1:11). Therefore, a two-step dilution (1:3, 1:2) was found to be the most suitable method and ratio for diluting the <i>Hu</i> ram semen after cryopreservation.https://www.mdpi.com/2076-2615/14/6/907dilution rates<i>Hu</i> ramcryopreservationspermatozoa motilityspermatozoa functional integrityspermatozoa ROS |
| spellingShingle | Liuming Zhang Xuyang Wang Caiyu Jiang Tariq Sohail Yuxuan Sun Xiaomei Sun Jian Wang Yongjun Li Effect of Different Dilution Methods and Ratios of Ram Semen on Sperm Parameters after Cryopreservation Animals dilution rates <i>Hu</i> ram cryopreservation spermatozoa motility spermatozoa functional integrity spermatozoa ROS |
| title | Effect of Different Dilution Methods and Ratios of Ram Semen on Sperm Parameters after Cryopreservation |
| title_full | Effect of Different Dilution Methods and Ratios of Ram Semen on Sperm Parameters after Cryopreservation |
| title_fullStr | Effect of Different Dilution Methods and Ratios of Ram Semen on Sperm Parameters after Cryopreservation |
| title_full_unstemmed | Effect of Different Dilution Methods and Ratios of Ram Semen on Sperm Parameters after Cryopreservation |
| title_short | Effect of Different Dilution Methods and Ratios of Ram Semen on Sperm Parameters after Cryopreservation |
| title_sort | effect of different dilution methods and ratios of ram semen on sperm parameters after cryopreservation |
| topic | dilution rates <i>Hu</i> ram cryopreservation spermatozoa motility spermatozoa functional integrity spermatozoa ROS |
| url | https://www.mdpi.com/2076-2615/14/6/907 |
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