Comparative evaluation of pH and Ca+ ion release from MTA on interaction with platelet-rich fibrin and blood clot: an in vitro study [version 2; peer review: 2 approved]
Background: ‘Regenerative endodontics’ using host-derived scaffolds and biomaterials (MTA) is popular in the management of teeth with open apex. Alkaline pH and bioactivity contribute to tissue healing and remineralization. We assessed the influence of PRF and Blood Clot on the pH and Ca+ ion releas...
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| Format: | Article |
| Language: | English |
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F1000 Research Ltd
2023-10-01
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| Online Access: | https://f1000research.com/articles/12-364/v2 |
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| author | Soumyadeep Ghosh Kavimalar Ravichandran Shruthi Nagaraja Swaroop Hegde Sonia Khatri Sylvia Mathew |
| author_facet | Soumyadeep Ghosh Kavimalar Ravichandran Shruthi Nagaraja Swaroop Hegde Sonia Khatri Sylvia Mathew |
| author_sort | Soumyadeep Ghosh |
| collection | DOAJ |
| description | Background: ‘Regenerative endodontics’ using host-derived scaffolds and biomaterials (MTA) is popular in the management of teeth with open apex. Alkaline pH and bioactivity contribute to tissue healing and remineralization. We assessed the influence of PRF and Blood Clot on the pH and Ca+ ion release from MTA. Methods: A total of 15 single-rooted human extracted teeth were sectioned at the level of the cementoenamel junction. Based on the type of scaffolds used, samples were divided into three groups. Group 1 (MTA+ PRF), Group 2 (MTA + Blood Clot), Group 3 (control MTA). The prepared specimens were transferred to a fresh falcon tube containing 10mL of distilled water and the collected solutions were analysed for pH and Ca+ ion release at 3h, seventh day and 14th day. Results: It was observed that the mean pH and Ca+ ion release were significantly lower in the experimental groups as compared to the control group. Though there was an increase in the pH recorded in Group 1 and 2 at all time periods, the difference was not significant. Ca+ ion release peaked at Day 7 (Group3 > Group2 > Group1) and reduced significantly on the 14th day for all groups. Conclusions: Within the limitations of the study, it can be concluded that PRF and blood clot influence the pH and Ca+ ion release from MTA. |
| first_indexed | 2024-03-10T10:13:33Z |
| format | Article |
| id | doaj.art-4ece0f2cc5d74720a1623683903719d2 |
| institution | Directory Open Access Journal |
| issn | 2046-1402 |
| language | English |
| last_indexed | 2024-03-10T10:13:33Z |
| publishDate | 2023-10-01 |
| publisher | F1000 Research Ltd |
| record_format | Article |
| series | F1000Research |
| spelling | doaj.art-4ece0f2cc5d74720a1623683903719d22023-11-22T01:00:00ZengF1000 Research LtdF1000Research2046-14022023-10-0112158056Comparative evaluation of pH and Ca+ ion release from MTA on interaction with platelet-rich fibrin and blood clot: an in vitro study [version 2; peer review: 2 approved]Soumyadeep Ghosh0https://orcid.org/0000-0001-6768-6332Kavimalar Ravichandran1Shruthi Nagaraja2Swaroop Hegde3Sonia Khatri4Sylvia Mathew5Conservative Dentistry and Endodontics, Manipal College of Dental Sciences, Manipal, Manipal Academy of Higher Education, Manipal, Karnataka, 576104, IndiaConservative Dentistry and Endodontics, Sri Ramakrishna Dental College and Hospital, Coimbatore, Coimbatore, Tamil Nadu, IndiaConservative Dentistry and Endodontics, Faculty of Dental Sciences, Ramaiah University of Applied Sciences, Bangalore, Karnataka, 560054, IndiaConservative Dentistry and Endodontics, Faculty of Dental Sciences, Ramaiah University of Applied Sciences, Bangalore, Karnataka, 560054, IndiaConservative Dentistry and Endodontics, Faculty of Dental Sciences, Ramaiah University of Applied Sciences, Bangalore, Karnataka, 560054, IndiaConservative Dentistry and Endodontics, Faculty of Dental Sciences, Ramaiah University of Applied Sciences, Bangalore, Karnataka, 560054, IndiaBackground: ‘Regenerative endodontics’ using host-derived scaffolds and biomaterials (MTA) is popular in the management of teeth with open apex. Alkaline pH and bioactivity contribute to tissue healing and remineralization. We assessed the influence of PRF and Blood Clot on the pH and Ca+ ion release from MTA. Methods: A total of 15 single-rooted human extracted teeth were sectioned at the level of the cementoenamel junction. Based on the type of scaffolds used, samples were divided into three groups. Group 1 (MTA+ PRF), Group 2 (MTA + Blood Clot), Group 3 (control MTA). The prepared specimens were transferred to a fresh falcon tube containing 10mL of distilled water and the collected solutions were analysed for pH and Ca+ ion release at 3h, seventh day and 14th day. Results: It was observed that the mean pH and Ca+ ion release were significantly lower in the experimental groups as compared to the control group. Though there was an increase in the pH recorded in Group 1 and 2 at all time periods, the difference was not significant. Ca+ ion release peaked at Day 7 (Group3 > Group2 > Group1) and reduced significantly on the 14th day for all groups. Conclusions: Within the limitations of the study, it can be concluded that PRF and blood clot influence the pH and Ca+ ion release from MTA.https://f1000research.com/articles/12-364/v2Regenerative Endodontics Mineral Trioxide Aggregate Platelet Rich Fibrineng |
| spellingShingle | Soumyadeep Ghosh Kavimalar Ravichandran Shruthi Nagaraja Swaroop Hegde Sonia Khatri Sylvia Mathew Comparative evaluation of pH and Ca+ ion release from MTA on interaction with platelet-rich fibrin and blood clot: an in vitro study [version 2; peer review: 2 approved] F1000Research Regenerative Endodontics Mineral Trioxide Aggregate Platelet Rich Fibrin eng |
| title | Comparative evaluation of pH and Ca+ ion release from MTA on interaction with platelet-rich fibrin and blood clot: an in vitro study [version 2; peer review: 2 approved] |
| title_full | Comparative evaluation of pH and Ca+ ion release from MTA on interaction with platelet-rich fibrin and blood clot: an in vitro study [version 2; peer review: 2 approved] |
| title_fullStr | Comparative evaluation of pH and Ca+ ion release from MTA on interaction with platelet-rich fibrin and blood clot: an in vitro study [version 2; peer review: 2 approved] |
| title_full_unstemmed | Comparative evaluation of pH and Ca+ ion release from MTA on interaction with platelet-rich fibrin and blood clot: an in vitro study [version 2; peer review: 2 approved] |
| title_short | Comparative evaluation of pH and Ca+ ion release from MTA on interaction with platelet-rich fibrin and blood clot: an in vitro study [version 2; peer review: 2 approved] |
| title_sort | comparative evaluation of ph and ca ion release from mta on interaction with platelet rich fibrin and blood clot an in vitro study version 2 peer review 2 approved |
| topic | Regenerative Endodontics Mineral Trioxide Aggregate Platelet Rich Fibrin eng |
| url | https://f1000research.com/articles/12-364/v2 |
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