Pig Coat Color Manipulation by <i>MC1R</i> Gene Editing
Black coat color in pigs is determined by the dominant <i>E</i> allele at the <i>MC1R</i> locus. Through comparing <i>MC1R</i> gene sequences between recessive <i>e</i> and dominant <i>E<sup>D1</sup></i> alleles, we identified f...
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MDPI AG
2022-09-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/23/18/10356 |
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| author | Haiwen Zhong Jian Zhang Cheng Tan Junsong Shi Jie Yang Gengyuan Cai Zhenfang Wu Huaqiang Yang |
| author_facet | Haiwen Zhong Jian Zhang Cheng Tan Junsong Shi Jie Yang Gengyuan Cai Zhenfang Wu Huaqiang Yang |
| author_sort | Haiwen Zhong |
| collection | DOAJ |
| description | Black coat color in pigs is determined by the dominant <i>E</i> allele at the <i>MC1R</i> locus. Through comparing <i>MC1R</i> gene sequences between recessive <i>e</i> and dominant <i>E<sup>D1</sup></i> alleles, we identified four missense mutations that could affect MC1R protein function for eumelanin synthesis. With the aim of devising a genetic modification method for pig coat color manipulation, we mutated the <i>e</i> allele in the Duroc breed to the dominant <i>E<sup>D1</sup></i> allele using CRISPR-mediated homologous recombination for the four mutation substitutions at the <i>MC1R</i> locus. The <i>MC1R</i>-modified Duroc pigs generated using the allele replacement strategy displayed uniform black coat color across the body. A genotyping assay showed that the <i>MC1R</i>-modified Duroc pigs had a heterozygous <i>E<sup>D1</sup></i>/<i>e</i> allele at the <i>MC1R</i> locus; in addition, the pigs remained in the Duroc genetic background. Our work offers a gene editing method for pig coat color manipulation, which could value the culture of new pig varieties meeting the needs of diversified market. |
| first_indexed | 2024-03-09T23:47:36Z |
| format | Article |
| id | doaj.art-4ecf958822d947a0935e9d2e8a4d2ce5 |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-09T23:47:36Z |
| publishDate | 2022-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-4ecf958822d947a0935e9d2e8a4d2ce52023-11-23T16:40:12ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672022-09-0123181035610.3390/ijms231810356Pig Coat Color Manipulation by <i>MC1R</i> Gene EditingHaiwen Zhong0Jian Zhang1Cheng Tan2Junsong Shi3Jie Yang4Gengyuan Cai5Zhenfang Wu6Huaqiang Yang7National Engineering Research Center for Breeding Swine Industry, College of Animal Science, South China Agricultural University, Guangzhou 510642, ChinaYunfu Branch Center of Guangdong Laboratory of Lingnan Modern Agricultural Science and Technology, Yunfu 527400, ChinaNational Engineering Research Center for Breeding Swine Industry, College of Animal Science, South China Agricultural University, Guangzhou 510642, ChinaNational Engineering Research Center for Breeding Swine Industry, College of Animal Science, South China Agricultural University, Guangzhou 510642, ChinaNational Engineering Research Center for Breeding Swine Industry, College of Animal Science, South China Agricultural University, Guangzhou 510642, ChinaNational Engineering Research Center for Breeding Swine Industry, College of Animal Science, South China Agricultural University, Guangzhou 510642, ChinaNational Engineering Research Center for Breeding Swine Industry, College of Animal Science, South China Agricultural University, Guangzhou 510642, ChinaNational Engineering Research Center for Breeding Swine Industry, College of Animal Science, South China Agricultural University, Guangzhou 510642, ChinaBlack coat color in pigs is determined by the dominant <i>E</i> allele at the <i>MC1R</i> locus. Through comparing <i>MC1R</i> gene sequences between recessive <i>e</i> and dominant <i>E<sup>D1</sup></i> alleles, we identified four missense mutations that could affect MC1R protein function for eumelanin synthesis. With the aim of devising a genetic modification method for pig coat color manipulation, we mutated the <i>e</i> allele in the Duroc breed to the dominant <i>E<sup>D1</sup></i> allele using CRISPR-mediated homologous recombination for the four mutation substitutions at the <i>MC1R</i> locus. The <i>MC1R</i>-modified Duroc pigs generated using the allele replacement strategy displayed uniform black coat color across the body. A genotyping assay showed that the <i>MC1R</i>-modified Duroc pigs had a heterozygous <i>E<sup>D1</sup></i>/<i>e</i> allele at the <i>MC1R</i> locus; in addition, the pigs remained in the Duroc genetic background. Our work offers a gene editing method for pig coat color manipulation, which could value the culture of new pig varieties meeting the needs of diversified market.https://www.mdpi.com/1422-0067/23/18/10356coat colorCRISPRgene editinghomologous recombination<i>MC1R</i>pig |
| spellingShingle | Haiwen Zhong Jian Zhang Cheng Tan Junsong Shi Jie Yang Gengyuan Cai Zhenfang Wu Huaqiang Yang Pig Coat Color Manipulation by <i>MC1R</i> Gene Editing International Journal of Molecular Sciences coat color CRISPR gene editing homologous recombination <i>MC1R</i> pig |
| title | Pig Coat Color Manipulation by <i>MC1R</i> Gene Editing |
| title_full | Pig Coat Color Manipulation by <i>MC1R</i> Gene Editing |
| title_fullStr | Pig Coat Color Manipulation by <i>MC1R</i> Gene Editing |
| title_full_unstemmed | Pig Coat Color Manipulation by <i>MC1R</i> Gene Editing |
| title_short | Pig Coat Color Manipulation by <i>MC1R</i> Gene Editing |
| title_sort | pig coat color manipulation by i mc1r i gene editing |
| topic | coat color CRISPR gene editing homologous recombination <i>MC1R</i> pig |
| url | https://www.mdpi.com/1422-0067/23/18/10356 |
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