Detection of Helicobacter pylori and its virulence genes (cagA, dupA, and vacA) among patients with gastroduodenal diseases in Chris Hani Baragwanath Academic Hospital, South Africa
Abstract Background The global prevalence of H. pylori approaches 50%, with prevalence rates between 20 and 40% in developed countries and up to 90% in Africa and other developing nations of the world. Development of H. pylori-associated diseases is determined by a number of virulence factors. This...
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| Format: | Article |
| Language: | English |
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BMC
2019-05-01
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| Series: | BMC Gastroenterology |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s12876-019-0986-0 |
| _version_ | 1828900551951122432 |
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| author | Ayodeji Idowu Asisipho Mzukwa Ute Harrison Pia Palamides Rainer Haas Melvin Mbao Razinah Mamdoo Jonathan Bolon Tolulope Jolaiya Stella Smith Reidwaan Ally Anna Clarke Henry Njom |
| author_facet | Ayodeji Idowu Asisipho Mzukwa Ute Harrison Pia Palamides Rainer Haas Melvin Mbao Razinah Mamdoo Jonathan Bolon Tolulope Jolaiya Stella Smith Reidwaan Ally Anna Clarke Henry Njom |
| author_sort | Ayodeji Idowu |
| collection | DOAJ |
| description | Abstract Background The global prevalence of H. pylori approaches 50%, with prevalence rates between 20 and 40% in developed countries and up to 90% in Africa and other developing nations of the world. Development of H. pylori-associated diseases is determined by a number of virulence factors. This study aimed at determining the prevalence of H. pylori infections and virulence genes (cagA, dupA, and vacA); the relationship between virulence factors and gastroduodenal diseases among patients. Methods Gastric biopsies were obtained from patients and cultured, DNA was extracted from cultured isolates and biopsies for PCR assay after which samples were investigated using standard laboratory procedures. Data of associated risk factors were obtained with the aid of questionnaires. Results Of the 444 participants, H. pylori was detected in 115 (25.9%) from culture analysis and 217 (48.9%) by direct PCR method. Ninety-eight (85.2%) of the culture-positive patients were also detected by PCR giving an overall prevalence of 52.7% (234/444). The highest number of H. pylori isolates 76.9% (180/234) was obtained from patients suffering from pangastritis. The CagA virulence gene was found in 62% (145/234), dupA in 53.4% (125/234) and vacA in 90.6% (212/234). VacA genotype s1 m1 was the most prevalent [56.4% (132)] followed by s2 m2 [11.5% (27)], s2 m1 [10.3% (24)] and [s1 m2 9.4% (22)]. There was a significant association observed in vacA s1 and peptic ulcer disease, as well as vacA s1/m2 and gastric erosion (P < 0.05). Conclusion The study revealed a significant association between virulence genes and the development of certain forms of gastric infections while the variations in H. pylori detection and the associated risk factors investigated in the study were not significantly related. |
| first_indexed | 2024-12-13T15:49:02Z |
| format | Article |
| id | doaj.art-4ed3665ee567437e9f59e9d7d6b969e2 |
| institution | Directory Open Access Journal |
| issn | 1471-230X |
| language | English |
| last_indexed | 2024-12-13T15:49:02Z |
| publishDate | 2019-05-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Gastroenterology |
| spelling | doaj.art-4ed3665ee567437e9f59e9d7d6b969e22022-12-21T23:39:35ZengBMCBMC Gastroenterology1471-230X2019-05-0119111010.1186/s12876-019-0986-0Detection of Helicobacter pylori and its virulence genes (cagA, dupA, and vacA) among patients with gastroduodenal diseases in Chris Hani Baragwanath Academic Hospital, South AfricaAyodeji Idowu0Asisipho Mzukwa1Ute Harrison2Pia Palamides3Rainer Haas4Melvin Mbao5Razinah Mamdoo6Jonathan Bolon7Tolulope Jolaiya8Stella Smith9Reidwaan Ally10Anna Clarke11Henry Njom12Department of Biochemistry and Microbiology, University of Fort HareDepartment of Biochemistry and Microbiology, University of Fort HareChair of Medical Microbiology and Hospital Epidemiology, Max von Pettenkofer-Institute, Faculty of Medicine, LMU MunichChair of Medical Microbiology and Hospital Epidemiology, Max von Pettenkofer-Institute, Faculty of Medicine, LMU MunichChair of Medical Microbiology and Hospital Epidemiology, Max von Pettenkofer-Institute, Faculty of Medicine, LMU MunichDivision of Gastroenterology, Chris Hani Baragwanath Academic Hospital (CHBAH)Division of Gastroenterology, Chris Hani Baragwanath Academic Hospital (CHBAH)Division of Gastroenterology, Chris Hani Baragwanath Academic Hospital (CHBAH)Department of Microbiology, University of LagosMolecular Biology Department, Nigerian Institute of Medical ResearchDivision of Gastroenterology, Chris Hani Baragwanath Academic Hospital (CHBAH)Department of Biochemistry and Microbiology, University of Fort HareDepartment of Biochemistry and Microbiology, University of Fort HareAbstract Background The global prevalence of H. pylori approaches 50%, with prevalence rates between 20 and 40% in developed countries and up to 90% in Africa and other developing nations of the world. Development of H. pylori-associated diseases is determined by a number of virulence factors. This study aimed at determining the prevalence of H. pylori infections and virulence genes (cagA, dupA, and vacA); the relationship between virulence factors and gastroduodenal diseases among patients. Methods Gastric biopsies were obtained from patients and cultured, DNA was extracted from cultured isolates and biopsies for PCR assay after which samples were investigated using standard laboratory procedures. Data of associated risk factors were obtained with the aid of questionnaires. Results Of the 444 participants, H. pylori was detected in 115 (25.9%) from culture analysis and 217 (48.9%) by direct PCR method. Ninety-eight (85.2%) of the culture-positive patients were also detected by PCR giving an overall prevalence of 52.7% (234/444). The highest number of H. pylori isolates 76.9% (180/234) was obtained from patients suffering from pangastritis. The CagA virulence gene was found in 62% (145/234), dupA in 53.4% (125/234) and vacA in 90.6% (212/234). VacA genotype s1 m1 was the most prevalent [56.4% (132)] followed by s2 m2 [11.5% (27)], s2 m1 [10.3% (24)] and [s1 m2 9.4% (22)]. There was a significant association observed in vacA s1 and peptic ulcer disease, as well as vacA s1/m2 and gastric erosion (P < 0.05). Conclusion The study revealed a significant association between virulence genes and the development of certain forms of gastric infections while the variations in H. pylori detection and the associated risk factors investigated in the study were not significantly related.http://link.springer.com/article/10.1186/s12876-019-0986-0H. pyloriPeptic ulcerPCR assayVirulence genescagAdupA |
| spellingShingle | Ayodeji Idowu Asisipho Mzukwa Ute Harrison Pia Palamides Rainer Haas Melvin Mbao Razinah Mamdoo Jonathan Bolon Tolulope Jolaiya Stella Smith Reidwaan Ally Anna Clarke Henry Njom Detection of Helicobacter pylori and its virulence genes (cagA, dupA, and vacA) among patients with gastroduodenal diseases in Chris Hani Baragwanath Academic Hospital, South Africa BMC Gastroenterology H. pylori Peptic ulcer PCR assay Virulence genes cagA dupA |
| title | Detection of Helicobacter pylori and its virulence genes (cagA, dupA, and vacA) among patients with gastroduodenal diseases in Chris Hani Baragwanath Academic Hospital, South Africa |
| title_full | Detection of Helicobacter pylori and its virulence genes (cagA, dupA, and vacA) among patients with gastroduodenal diseases in Chris Hani Baragwanath Academic Hospital, South Africa |
| title_fullStr | Detection of Helicobacter pylori and its virulence genes (cagA, dupA, and vacA) among patients with gastroduodenal diseases in Chris Hani Baragwanath Academic Hospital, South Africa |
| title_full_unstemmed | Detection of Helicobacter pylori and its virulence genes (cagA, dupA, and vacA) among patients with gastroduodenal diseases in Chris Hani Baragwanath Academic Hospital, South Africa |
| title_short | Detection of Helicobacter pylori and its virulence genes (cagA, dupA, and vacA) among patients with gastroduodenal diseases in Chris Hani Baragwanath Academic Hospital, South Africa |
| title_sort | detection of helicobacter pylori and its virulence genes caga dupa and vaca among patients with gastroduodenal diseases in chris hani baragwanath academic hospital south africa |
| topic | H. pylori Peptic ulcer PCR assay Virulence genes cagA dupA |
| url | http://link.springer.com/article/10.1186/s12876-019-0986-0 |
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