Participation of NO in the vasodilatory action of isoespintanol
Background: accumulating evidence suggests that natural compounds and specifically monoterpenes exert a vasodilator action. Objetive: to investigate the vascular effects of isoespintanol (2-isopropil-3,6- dimetoxi-5-metilfenol, ISO) monoterpene isolated from the leaves of Oxandra cf xylopioides. Met...
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| Format: | Article |
| Language: | English |
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Universidad de Antioquia
2019-11-01
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| Series: | Vitae |
| Subjects: | |
| Online Access: | https://revistas.udea.edu.co/index.php/vitae/article/view/336274 |
| _version_ | 1818906416337911808 |
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| author | Gustavo J. RINALDI Benjamín ROJANO Guillermo SCHINELLA Susana M. MOSCA |
| author_facet | Gustavo J. RINALDI Benjamín ROJANO Guillermo SCHINELLA Susana M. MOSCA |
| author_sort | Gustavo J. RINALDI |
| collection | DOAJ |
| description | Background: accumulating evidence suggests that natural compounds and specifically monoterpenes exert a vasodilator action. Objetive: to investigate the vascular effects of isoespintanol (2-isopropil-3,6- dimetoxi-5-metilfenol, ISO) monoterpene isolated from the leaves of Oxandra cf xylopioides. Methods: thoracic aortic rings isolated from Wistar rats were contracted with KCl 80 mM and then relaxed by exposure to Ca2+-free solution in absence and in presence of ISO 0.6 mg/mL. The force/tissue ratio (F/W) and the time to obtain 50% of relaxation (T-50) were used to assess the maximal contractile response and the relaxation, respectively. To examine the participation of NO additional experiments were performed under inhibition of nitric oxide synthase with L-NAME (L-NG-Nitroarginine methyl ester). Results: ISO significantly decreased the F/W ratio (257 ± 19 vs. 360 ± 18) and did not change T-50. In presence of L-NAME the effects of ISO on contractile response was abolished. Conclusions: these results demonstrate that ISO exerts a vasodilator effect through NO- dependent pathways and suggest that an inhibition of calcium influx could be the involved mechanism. |
| first_indexed | 2024-12-19T21:38:53Z |
| format | Article |
| id | doaj.art-4ed51b4f6f104edcbff4e9c3d0c55a03 |
| institution | Directory Open Access Journal |
| issn | 0121-4004 2145-2660 |
| language | English |
| last_indexed | 2024-12-19T21:38:53Z |
| publishDate | 2019-11-01 |
| publisher | Universidad de Antioquia |
| record_format | Article |
| series | Vitae |
| spelling | doaj.art-4ed51b4f6f104edcbff4e9c3d0c55a032022-12-21T20:04:42ZengUniversidad de AntioquiaVitae0121-40042145-26602019-11-0126210.17533/udea.vitae.v26n2a03Participation of NO in the vasodilatory action of isoespintanolGustavo J. RINALDI0Benjamín ROJANO1Guillermo SCHINELLA2Susana M. MOSCA3Universidad Nacional de la PlataUniversidad Nacional de Colombia Universidad Nacional de La PlataUniversidad Nacional de La PlataBackground: accumulating evidence suggests that natural compounds and specifically monoterpenes exert a vasodilator action. Objetive: to investigate the vascular effects of isoespintanol (2-isopropil-3,6- dimetoxi-5-metilfenol, ISO) monoterpene isolated from the leaves of Oxandra cf xylopioides. Methods: thoracic aortic rings isolated from Wistar rats were contracted with KCl 80 mM and then relaxed by exposure to Ca2+-free solution in absence and in presence of ISO 0.6 mg/mL. The force/tissue ratio (F/W) and the time to obtain 50% of relaxation (T-50) were used to assess the maximal contractile response and the relaxation, respectively. To examine the participation of NO additional experiments were performed under inhibition of nitric oxide synthase with L-NAME (L-NG-Nitroarginine methyl ester). Results: ISO significantly decreased the F/W ratio (257 ± 19 vs. 360 ± 18) and did not change T-50. In presence of L-NAME the effects of ISO on contractile response was abolished. Conclusions: these results demonstrate that ISO exerts a vasodilator effect through NO- dependent pathways and suggest that an inhibition of calcium influx could be the involved mechanism.https://revistas.udea.edu.co/index.php/vitae/article/view/336274Isoespintanolthoracic aortanitric oxide (NO). |
| spellingShingle | Gustavo J. RINALDI Benjamín ROJANO Guillermo SCHINELLA Susana M. MOSCA Participation of NO in the vasodilatory action of isoespintanol Vitae Isoespintanol thoracic aorta nitric oxide (NO). |
| title | Participation of NO in the vasodilatory action of isoespintanol |
| title_full | Participation of NO in the vasodilatory action of isoespintanol |
| title_fullStr | Participation of NO in the vasodilatory action of isoespintanol |
| title_full_unstemmed | Participation of NO in the vasodilatory action of isoespintanol |
| title_short | Participation of NO in the vasodilatory action of isoespintanol |
| title_sort | participation of no in the vasodilatory action of isoespintanol |
| topic | Isoespintanol thoracic aorta nitric oxide (NO). |
| url | https://revistas.udea.edu.co/index.php/vitae/article/view/336274 |
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