Planctoellipticum variicoloris gen. nov., sp. nov., a novel member of the family Planctomycetaceae isolated from wastewater of the aeration lagoon of a sugar processing plant in Northern Germany
Abstract In the present study, we characterise a strain isolated from the wastewater aeration lagoon of a sugar processing plant in Schleswig (Northern Germany) by Heinz Schlesner. As a pioneer in planctomycetal research, he isolated numerous strains belonging to the phylum Planctomycetota from aqua...
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Nature Portfolio
2024-03-01
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| Series: | Scientific Reports |
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| Online Access: | https://doi.org/10.1038/s41598-024-56373-y |
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| author | Carmen E. Wurzbacher Tom Haufschild Jonathan Hammer Muriel C. F. van Teeseling Nicolai Kallscheuer Christian Jogler |
| author_facet | Carmen E. Wurzbacher Tom Haufschild Jonathan Hammer Muriel C. F. van Teeseling Nicolai Kallscheuer Christian Jogler |
| author_sort | Carmen E. Wurzbacher |
| collection | DOAJ |
| description | Abstract In the present study, we characterise a strain isolated from the wastewater aeration lagoon of a sugar processing plant in Schleswig (Northern Germany) by Heinz Schlesner. As a pioneer in planctomycetal research, he isolated numerous strains belonging to the phylum Planctomycetota from aquatic habitats around the world. Phylogenetic analyses show that strain SH412T belongs to the family Planctomycetaceae and shares with 91.6% the highest 16S rRNA gene sequence similarity with Planctopirus limnophila DSM 3776T. Its genome has a length of 7.3 Mb and a G + C content of 63.6%. Optimal growth of strain SH412T occurs at pH 7.0–7.5 and 28 °C with its pigmentation depending on sunlight exposure. Strain SH412T reproduces by polar asymmetric division (“budding”) and forms ovoid cells. The cell size determination was performed using a semi-automatic pipeline, which we first evaluated with the model species P. limnophila and then applied to strain SH412T. Furthermore, the data acquired during time-lapse analyses suggests a lifestyle switch from flagellated daughter cells to non-flagellated mother cells in the subsequent cycle. Based on our data, we suggest that strain SH412T represents a novel species within a novel genus, for which we propose the name Planctoellipticum variicoloris gen. nov., sp. nov., with strain SH412T (= CECT 30430T = STH00996T, the STH number refers to the Jena Microbial Resource Collection JMRC) as the type strain of the new species. |
| first_indexed | 2024-04-25T01:05:51Z |
| format | Article |
| id | doaj.art-4ed51c0609e14890ae8751d8a1ab5cc9 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-04-25T01:05:51Z |
| publishDate | 2024-03-01 |
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| series | Scientific Reports |
| spelling | doaj.art-4ed51c0609e14890ae8751d8a1ab5cc92024-03-10T12:12:35ZengNature PortfolioScientific Reports2045-23222024-03-0114111410.1038/s41598-024-56373-yPlanctoellipticum variicoloris gen. nov., sp. nov., a novel member of the family Planctomycetaceae isolated from wastewater of the aeration lagoon of a sugar processing plant in Northern GermanyCarmen E. Wurzbacher0Tom Haufschild1Jonathan Hammer2Muriel C. F. van Teeseling3Nicolai Kallscheuer4Christian Jogler5Department of Microbial Interactions, Institute of Microbiology, Friedrich Schiller UniversityDepartment of Microbial Interactions, Institute of Microbiology, Friedrich Schiller UniversityDepartment of Microbial Interactions, Institute of Microbiology, Friedrich Schiller UniversityJunior Research Group “Prokaryotic Cell Biology”, Institute of Microbiology, Friedrich Schiller UniversityDepartment of Microbial Interactions, Institute of Microbiology, Friedrich Schiller UniversityDepartment of Microbial Interactions, Institute of Microbiology, Friedrich Schiller UniversityAbstract In the present study, we characterise a strain isolated from the wastewater aeration lagoon of a sugar processing plant in Schleswig (Northern Germany) by Heinz Schlesner. As a pioneer in planctomycetal research, he isolated numerous strains belonging to the phylum Planctomycetota from aquatic habitats around the world. Phylogenetic analyses show that strain SH412T belongs to the family Planctomycetaceae and shares with 91.6% the highest 16S rRNA gene sequence similarity with Planctopirus limnophila DSM 3776T. Its genome has a length of 7.3 Mb and a G + C content of 63.6%. Optimal growth of strain SH412T occurs at pH 7.0–7.5 and 28 °C with its pigmentation depending on sunlight exposure. Strain SH412T reproduces by polar asymmetric division (“budding”) and forms ovoid cells. The cell size determination was performed using a semi-automatic pipeline, which we first evaluated with the model species P. limnophila and then applied to strain SH412T. Furthermore, the data acquired during time-lapse analyses suggests a lifestyle switch from flagellated daughter cells to non-flagellated mother cells in the subsequent cycle. Based on our data, we suggest that strain SH412T represents a novel species within a novel genus, for which we propose the name Planctoellipticum variicoloris gen. nov., sp. nov., with strain SH412T (= CECT 30430T = STH00996T, the STH number refers to the Jena Microbial Resource Collection JMRC) as the type strain of the new species.https://doi.org/10.1038/s41598-024-56373-yLimnic bacteriaPlanctomycetotaAsymmetric cell divisionSchlesner strain collectionPigmentation |
| spellingShingle | Carmen E. Wurzbacher Tom Haufschild Jonathan Hammer Muriel C. F. van Teeseling Nicolai Kallscheuer Christian Jogler Planctoellipticum variicoloris gen. nov., sp. nov., a novel member of the family Planctomycetaceae isolated from wastewater of the aeration lagoon of a sugar processing plant in Northern Germany Scientific Reports Limnic bacteria Planctomycetota Asymmetric cell division Schlesner strain collection Pigmentation |
| title | Planctoellipticum variicoloris gen. nov., sp. nov., a novel member of the family Planctomycetaceae isolated from wastewater of the aeration lagoon of a sugar processing plant in Northern Germany |
| title_full | Planctoellipticum variicoloris gen. nov., sp. nov., a novel member of the family Planctomycetaceae isolated from wastewater of the aeration lagoon of a sugar processing plant in Northern Germany |
| title_fullStr | Planctoellipticum variicoloris gen. nov., sp. nov., a novel member of the family Planctomycetaceae isolated from wastewater of the aeration lagoon of a sugar processing plant in Northern Germany |
| title_full_unstemmed | Planctoellipticum variicoloris gen. nov., sp. nov., a novel member of the family Planctomycetaceae isolated from wastewater of the aeration lagoon of a sugar processing plant in Northern Germany |
| title_short | Planctoellipticum variicoloris gen. nov., sp. nov., a novel member of the family Planctomycetaceae isolated from wastewater of the aeration lagoon of a sugar processing plant in Northern Germany |
| title_sort | planctoellipticum variicoloris gen nov sp nov a novel member of the family planctomycetaceae isolated from wastewater of the aeration lagoon of a sugar processing plant in northern germany |
| topic | Limnic bacteria Planctomycetota Asymmetric cell division Schlesner strain collection Pigmentation |
| url | https://doi.org/10.1038/s41598-024-56373-y |
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