Effect of emodin on growth performance, antioxidant status, intestinal morphology and heat shock protein gene expressions of freshwater prawn (Macrobrachium rosenbergii)
The purpose of this study was to assess the effect of emodin on biochemical variables, antioxidant activity, heat shock protein gene expression and prawn intestinal morphology (Macrobrachium rosenbergii). Three feeds containing 0 mg kg–1 (T1, control group), 25 mg kg–1 (T2) and 50 mg kg–1 (T3) of e...
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| Format: | Article |
| Language: | English |
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BdFISH
2022-12-01
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| Series: | Journal of Fisheries |
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| Online Access: | https://journal.bdfish.org/index.php/fisheries/article/view/436 |
| _version_ | 1828083917354172416 |
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| author | Dawit Adisu Tadese Gashaw Tesfaye Metekia Tamiru Lishan Alemu Fikremariam Geda |
| author_facet | Dawit Adisu Tadese Gashaw Tesfaye Metekia Tamiru Lishan Alemu Fikremariam Geda |
| author_sort | Dawit Adisu Tadese |
| collection | DOAJ |
| description |
The purpose of this study was to assess the effect of emodin on biochemical variables, antioxidant activity, heat shock protein gene expression and prawn intestinal morphology (Macrobrachium rosenbergii). Three feeds containing 0 mg kg–1 (T1, control group), 25 mg kg–1 (T2) and 50 mg kg–1 (T3) of emodin were formulated and fed for eight weeks. Alanine aminotransferase, total cholesterol and triglyceride contents were significantly lowered although glucose tended to increase following the emodin remedy. Superoxide dismutase variables in haemolymph were dramatically upgraded, whilst malondialdehyde and superoxide anion situations were significantly lowered when compared to control. Emodin supplementation had no influence on glutathione peroxidase contents. Emodin remedy dropped the expression of HSP60 and HSP90 genes in comparison to the control. In T2, the intestinal epithelial cells were complete, well-organised and tightly affixed to the basement membrane, in discrepancy to the slightly disunited basement membrane in the control group. The emodin-treated prawn individuals had a thicker and full intestinal morphology than the control groups. Supplementation with emodin at dosages of 25 mg kg–1 improved growth performance, blood biochemical, antioxidant capacity and immune gene expression.
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| first_indexed | 2024-04-11T04:15:04Z |
| format | Article |
| id | doaj.art-4ed56f133e2a4e8db64bdaee8998ac1c |
| institution | Directory Open Access Journal |
| issn | 2311-729X 2311-3111 |
| language | English |
| last_indexed | 2024-04-11T04:15:04Z |
| publishDate | 2022-12-01 |
| publisher | BdFISH |
| record_format | Article |
| series | Journal of Fisheries |
| spelling | doaj.art-4ed56f133e2a4e8db64bdaee8998ac1c2022-12-31T18:52:11ZengBdFISHJournal of Fisheries2311-729X2311-31112022-12-0110310.17017/j.fish.436Effect of emodin on growth performance, antioxidant status, intestinal morphology and heat shock protein gene expressions of freshwater prawn (Macrobrachium rosenbergii)Dawit Adisu Tadese0Gashaw Tesfaye1Metekia Tamiru2Lishan Alemu3Fikremariam Geda4Wuxi Fisheries College, Nanjing Agricultural University, Wuxi 214081, China; Key Laboratory of Animal Models and Human Disease Mechanisms of the Chinese Academy of Sciences, Kunming Institute of Zoology, Kunming 650107, China; Ethiopian Institute of Agricultural Research, National Fishery, and other Aquatic Life Research Center, P. O. Box 64, Sebeta, EthiopiaEthiopian Institute of Agricultural Research, National Fishery, and other Aquatic Life Research Center, P. O. Box 64, Sebeta, Ethiopia; Institute of Hydrobiology, Biology Centre CAS, Ceske Budejovice, Czech RepublicDepartment of Animal Science, Jimma University College of Agriculture and Veterinary Medicine, P. O. Box 307, Jimma, Ethiopia; Ghent University, Faculty of Veterinary Medicine, Department of Nutrition, Genetics and Ethology, Heidestraat 19, B-9820, Merelbeke, BelgiumDepartment of Animal Science, Jimma University College of Agriculture and Veterinary Medicine, P. O. Box 307, Jimma, EthiopiaDepartment of Animal Science, Jimma University College of Agriculture and Veterinary Medicine, P. O. Box 307, Jimma, Ethiopia The purpose of this study was to assess the effect of emodin on biochemical variables, antioxidant activity, heat shock protein gene expression and prawn intestinal morphology (Macrobrachium rosenbergii). Three feeds containing 0 mg kg–1 (T1, control group), 25 mg kg–1 (T2) and 50 mg kg–1 (T3) of emodin were formulated and fed for eight weeks. Alanine aminotransferase, total cholesterol and triglyceride contents were significantly lowered although glucose tended to increase following the emodin remedy. Superoxide dismutase variables in haemolymph were dramatically upgraded, whilst malondialdehyde and superoxide anion situations were significantly lowered when compared to control. Emodin supplementation had no influence on glutathione peroxidase contents. Emodin remedy dropped the expression of HSP60 and HSP90 genes in comparison to the control. In T2, the intestinal epithelial cells were complete, well-organised and tightly affixed to the basement membrane, in discrepancy to the slightly disunited basement membrane in the control group. The emodin-treated prawn individuals had a thicker and full intestinal morphology than the control groups. Supplementation with emodin at dosages of 25 mg kg–1 improved growth performance, blood biochemical, antioxidant capacity and immune gene expression. https://journal.bdfish.org/index.php/fisheries/article/view/436antioxidantemodinheat shock proteinintestinal epithelial cellsMacrobrachium rosenbergii |
| spellingShingle | Dawit Adisu Tadese Gashaw Tesfaye Metekia Tamiru Lishan Alemu Fikremariam Geda Effect of emodin on growth performance, antioxidant status, intestinal morphology and heat shock protein gene expressions of freshwater prawn (Macrobrachium rosenbergii) Journal of Fisheries antioxidant emodin heat shock protein intestinal epithelial cells Macrobrachium rosenbergii |
| title | Effect of emodin on growth performance, antioxidant status, intestinal morphology and heat shock protein gene expressions of freshwater prawn (Macrobrachium rosenbergii) |
| title_full | Effect of emodin on growth performance, antioxidant status, intestinal morphology and heat shock protein gene expressions of freshwater prawn (Macrobrachium rosenbergii) |
| title_fullStr | Effect of emodin on growth performance, antioxidant status, intestinal morphology and heat shock protein gene expressions of freshwater prawn (Macrobrachium rosenbergii) |
| title_full_unstemmed | Effect of emodin on growth performance, antioxidant status, intestinal morphology and heat shock protein gene expressions of freshwater prawn (Macrobrachium rosenbergii) |
| title_short | Effect of emodin on growth performance, antioxidant status, intestinal morphology and heat shock protein gene expressions of freshwater prawn (Macrobrachium rosenbergii) |
| title_sort | effect of emodin on growth performance antioxidant status intestinal morphology and heat shock protein gene expressions of freshwater prawn macrobrachium rosenbergii |
| topic | antioxidant emodin heat shock protein intestinal epithelial cells Macrobrachium rosenbergii |
| url | https://journal.bdfish.org/index.php/fisheries/article/view/436 |
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