Preliminary Researches of Sperm Cryopreservation on Poliodon (Polyodon spathula) for Optimization of Spawning Biotechnology
The paper presents the first experiments of sperm cryopreservation on poliodon (Polyodon spathula), recently acclimated sturgeon in Romania. The accomplished research has been to determine the effect of using methanol (MeOH) and dimethyl sulfoxide (DMSO) as cryoprotectants in combination with two ex...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Agroprint Timisoara
2023-09-01
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| Series: | Scientific Papers Animal Science and Biotechnologies |
| Subjects: | |
| Online Access: | https://spasb.ro/index.php/public_html/article/view/657 |
| _version_ | 1797215017022521344 |
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| author | Mioara Costache Daniela Radu Daniel Oprea Mihail Costache Cecilia Bucur Carmen Georgeta Nicolae |
| author_facet | Mioara Costache Daniela Radu Daniel Oprea Mihail Costache Cecilia Bucur Carmen Georgeta Nicolae |
| author_sort | Mioara Costache |
| collection | DOAJ |
| description | The paper presents the first experiments of sperm cryopreservation on poliodon (Polyodon spathula), recently acclimated sturgeon in Romania. The accomplished research has been to determine the effect of using methanol (MeOH) and dimethyl sulfoxide (DMSO) as cryoprotectants in combination with two extenders (modified Tsvetkova’s extender: mT and modified Hanks’ balanced salt solution: mHBSS) on the spermatozoa motility and fertilization and hatching percentage of eggs by use of thawed sperm.
The best post thawed motility (78±5%) was recorded using modified Tsvetkova’s extender: mT with 1% MeOH, and the best fertilization and hatching percentage of eggs were achieved by using mT extender in combination with 5% MeOH. The results confirm data from literature (Detlaf et al. 1993, Mims et al. 2000, Akos Horvath et al. 2006) reporting that MeOH is a proper cryoprotectant for freezing poliodon sperm. |
| first_indexed | 2024-04-24T11:23:22Z |
| format | Article |
| id | doaj.art-4ed68cf8faf54eaf8e138aa0d9ab92fd |
| institution | Directory Open Access Journal |
| issn | 1841-9364 2344-4576 |
| language | English |
| last_indexed | 2024-04-24T11:23:22Z |
| publishDate | 2023-09-01 |
| publisher | Agroprint Timisoara |
| record_format | Article |
| series | Scientific Papers Animal Science and Biotechnologies |
| spelling | doaj.art-4ed68cf8faf54eaf8e138aa0d9ab92fd2024-04-11T03:50:09ZengAgroprint TimisoaraScientific Papers Animal Science and Biotechnologies1841-93642344-45762023-09-014522727657Preliminary Researches of Sperm Cryopreservation on Poliodon (Polyodon spathula) for Optimization of Spawning BiotechnologyMioara Costache0Daniela Radu1Daniel Oprea2Mihail Costache3Cecilia Bucur4Carmen Georgeta Nicolae5Fish Culture Research and Development Station Nucet, 137335–Nucet, Romania Fish Culture Research and Development Station Nucet, 137335–Nucet, Romania Fish Culture Research and Development Station Nucet, 137335–Nucet, Romania Fish Culture Research and Development Station Nucet, 137335–Nucet, Romania Fish Culture Research and Development Station Nucet, 137335–Nucet, Romania University of Agricultural Sciences and Veterinary Medicine Bucharest, 011463–Bucuresti, B-dul Marasti, 51, RomaniaThe paper presents the first experiments of sperm cryopreservation on poliodon (Polyodon spathula), recently acclimated sturgeon in Romania. The accomplished research has been to determine the effect of using methanol (MeOH) and dimethyl sulfoxide (DMSO) as cryoprotectants in combination with two extenders (modified Tsvetkova’s extender: mT and modified Hanks’ balanced salt solution: mHBSS) on the spermatozoa motility and fertilization and hatching percentage of eggs by use of thawed sperm. The best post thawed motility (78±5%) was recorded using modified Tsvetkova’s extender: mT with 1% MeOH, and the best fertilization and hatching percentage of eggs were achieved by using mT extender in combination with 5% MeOH. The results confirm data from literature (Detlaf et al. 1993, Mims et al. 2000, Akos Horvath et al. 2006) reporting that MeOH is a proper cryoprotectant for freezing poliodon sperm.https://spasb.ro/index.php/public_html/article/view/657cryopreservationfertilizationmotilitypoliodonspawn |
| spellingShingle | Mioara Costache Daniela Radu Daniel Oprea Mihail Costache Cecilia Bucur Carmen Georgeta Nicolae Preliminary Researches of Sperm Cryopreservation on Poliodon (Polyodon spathula) for Optimization of Spawning Biotechnology Scientific Papers Animal Science and Biotechnologies cryopreservation fertilization motility poliodon spawn |
| title | Preliminary Researches of Sperm Cryopreservation on Poliodon (Polyodon spathula) for Optimization of Spawning Biotechnology |
| title_full | Preliminary Researches of Sperm Cryopreservation on Poliodon (Polyodon spathula) for Optimization of Spawning Biotechnology |
| title_fullStr | Preliminary Researches of Sperm Cryopreservation on Poliodon (Polyodon spathula) for Optimization of Spawning Biotechnology |
| title_full_unstemmed | Preliminary Researches of Sperm Cryopreservation on Poliodon (Polyodon spathula) for Optimization of Spawning Biotechnology |
| title_short | Preliminary Researches of Sperm Cryopreservation on Poliodon (Polyodon spathula) for Optimization of Spawning Biotechnology |
| title_sort | preliminary researches of sperm cryopreservation on poliodon polyodon spathula for optimization of spawning biotechnology |
| topic | cryopreservation fertilization motility poliodon spawn |
| url | https://spasb.ro/index.php/public_html/article/view/657 |
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