Response surface optimization of microwave-assisted extraction for HPLC-fluorescence determination of puerarin and daidzein in Radix Puerariae thomsonii
Microwave-assisted extraction was optimized with response surface methodology for HPLC-fluorescence determination of puerarin and daidzein in Radix Puerariae thomsonii. The optimized extraction procedure was achieved by soaking the sample with 70% methanol (1:15, v/v) for 30 min, and then microwave...
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| Format: | Article |
| Language: | English |
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Elsevier
2011-02-01
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| Series: | Journal of Pharmaceutical Analysis |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S209517791170003X |
| _version_ | 1818351612553330688 |
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| author | Ying-Kun Liu E. Yan Han-Ying Zhan Zhi-Qi Zhang |
| author_facet | Ying-Kun Liu E. Yan Han-Ying Zhan Zhi-Qi Zhang |
| author_sort | Ying-Kun Liu |
| collection | DOAJ |
| description | Microwave-assisted extraction was optimized with response surface methodology for HPLC-fluorescence determination of puerarin and daidzein in Radix Puerariae thomsonii. The optimized extraction procedure was achieved by soaking the sample with 70% methanol (1:15, v/v) for 30 min, and then microwave irradiation for 11 min at a power of 600 W. Coupling the extraction process with HPLC-fluorescence presented good recovery, satisfactory precision, and good linear relation. Compared with a method from the Chinese Pharmacopoeia, the proposed method enables higher extraction efficiency and more aecurate analytical results. It can be of Potential value in quality assessment of Radix Puerariae thomsonii medicinal materials. Keywords: microwave-assisted extraction (MAE), response surface methodology (RSM), puerarin, daidzein, HPLC-fluorescence detection, Radix Puerariae thomsonii |
| first_indexed | 2024-12-13T18:40:31Z |
| format | Article |
| id | doaj.art-4ed7b25ed03f4b7ab9992a54bdbd1115 |
| institution | Directory Open Access Journal |
| issn | 2095-1779 |
| language | English |
| last_indexed | 2024-12-13T18:40:31Z |
| publishDate | 2011-02-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Pharmaceutical Analysis |
| spelling | doaj.art-4ed7b25ed03f4b7ab9992a54bdbd11152022-12-21T23:35:15ZengElsevierJournal of Pharmaceutical Analysis2095-17792011-02-01111319Response surface optimization of microwave-assisted extraction for HPLC-fluorescence determination of puerarin and daidzein in Radix Puerariae thomsoniiYing-Kun Liu0E. Yan1Han-Ying Zhan2Zhi-Qi Zhang3Key Laboratory of Medicinal Resource and Natural Pharmaceutical Chemistry (Shaanxi Normal University), Ministry of Education, Xiâan 710062, China; Nurturing Station for the State Key Laboratory of Subtropical Silviculture, Zhejiang Forestry University, Linâan 311300, ChinaKey Laboratory of Medicinal Resource and Natural Pharmaceutical Chemistry (Shaanxi Normal University), Ministry of Education, Xiâan 710062, China; Department of Chemistry, Qinghai Normal University, Xining 810008, ChinaKey Laboratory of Medicinal Resource and Natural Pharmaceutical Chemistry (Shaanxi Normal University), Ministry of Education, Xiâan 710062, ChinaKey Laboratory of Medicinal Resource and Natural Pharmaceutical Chemistry (Shaanxi Normal University), Ministry of Education, Xiâan 710062, China; Corresponding author.Microwave-assisted extraction was optimized with response surface methodology for HPLC-fluorescence determination of puerarin and daidzein in Radix Puerariae thomsonii. The optimized extraction procedure was achieved by soaking the sample with 70% methanol (1:15, v/v) for 30 min, and then microwave irradiation for 11 min at a power of 600 W. Coupling the extraction process with HPLC-fluorescence presented good recovery, satisfactory precision, and good linear relation. Compared with a method from the Chinese Pharmacopoeia, the proposed method enables higher extraction efficiency and more aecurate analytical results. It can be of Potential value in quality assessment of Radix Puerariae thomsonii medicinal materials. Keywords: microwave-assisted extraction (MAE), response surface methodology (RSM), puerarin, daidzein, HPLC-fluorescence detection, Radix Puerariae thomsoniihttp://www.sciencedirect.com/science/article/pii/S209517791170003X |
| spellingShingle | Ying-Kun Liu E. Yan Han-Ying Zhan Zhi-Qi Zhang Response surface optimization of microwave-assisted extraction for HPLC-fluorescence determination of puerarin and daidzein in Radix Puerariae thomsonii Journal of Pharmaceutical Analysis |
| title | Response surface optimization of microwave-assisted extraction for HPLC-fluorescence determination of puerarin and daidzein in Radix Puerariae thomsonii |
| title_full | Response surface optimization of microwave-assisted extraction for HPLC-fluorescence determination of puerarin and daidzein in Radix Puerariae thomsonii |
| title_fullStr | Response surface optimization of microwave-assisted extraction for HPLC-fluorescence determination of puerarin and daidzein in Radix Puerariae thomsonii |
| title_full_unstemmed | Response surface optimization of microwave-assisted extraction for HPLC-fluorescence determination of puerarin and daidzein in Radix Puerariae thomsonii |
| title_short | Response surface optimization of microwave-assisted extraction for HPLC-fluorescence determination of puerarin and daidzein in Radix Puerariae thomsonii |
| title_sort | response surface optimization of microwave assisted extraction for hplc fluorescence determination of puerarin and daidzein in radix puerariae thomsonii |
| url | http://www.sciencedirect.com/science/article/pii/S209517791170003X |
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