Application of laccase and hydrolases for trace organic contaminants removal from contaminated water
The potential of a laccase from Trametes hirsuta, a hydrolase mixture of amylase, lipase cellulase and protease, was studied for the removal of a set of trace organic contaminants (TrOCs) at 10 µg/L each in a contaminated water. An attempt was also made to develop a combination of cross-linked enzym...
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2022-07-01
|
| Series: | Environmental Advances |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666765722000783 |
| _version_ | 1811250294128902144 |
|---|---|
| author | Komla Alokpa François Lafortune Hubert Cabana |
| author_facet | Komla Alokpa François Lafortune Hubert Cabana |
| author_sort | Komla Alokpa |
| collection | DOAJ |
| description | The potential of a laccase from Trametes hirsuta, a hydrolase mixture of amylase, lipase cellulase and protease, was studied for the removal of a set of trace organic contaminants (TrOCs) at 10 µg/L each in a contaminated water. An attempt was also made to develop a combination of cross-linked enzyme aggregates (combi-CLEA) from the two groups of enzymes. After a 24-hour in batch treatments at 22°C and pH 7, compounds such as acetaminophen and levothyroxine showed a decrease in concentration of 97% and 99% respectively, in presence of laccase, while amlodipine, levothyroxine, and trimethoprim were removed at 98%, 99% and 99% respectively, when the contaminated water was treated with the hydrolase mixture. The study of the impact of pH, temperature and contact time revealed that the most favorable temperatures were 22°C and 30°C, but no clear correlation was found between pH and removal in the pH range of 6-8, although high removals were achieved at these pHs. Finally, except few compounds such as acetaminophen, the TrOC removal after 24 h was higher than that obtained in 4 h. |
| first_indexed | 2024-04-12T16:01:19Z |
| format | Article |
| id | doaj.art-4edc594789384006b8aa2633002d947a |
| institution | Directory Open Access Journal |
| issn | 2666-7657 |
| language | English |
| last_indexed | 2024-04-12T16:01:19Z |
| publishDate | 2022-07-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Environmental Advances |
| spelling | doaj.art-4edc594789384006b8aa2633002d947a2022-12-22T03:26:11ZengElsevierEnvironmental Advances2666-76572022-07-018100243Application of laccase and hydrolases for trace organic contaminants removal from contaminated waterKomla Alokpa0François Lafortune1Hubert Cabana2Université de Sherbrooke Water Research Group, Department of Civil and Building Engineering, Université de Sherbrooke, Sherbrooke Québec, Canada; Centre universitaire de formation en environnement et développement durable, Université de Sherbrooke, Sherbrooke Québec, CanadaCentre universitaire de formation en environnement et développement durable, Université de Sherbrooke, Sherbrooke Québec, CanadaUniversité de Sherbrooke Water Research Group, Department of Civil and Building Engineering, Université de Sherbrooke, Sherbrooke Québec, Canada; Corresponding author at: Department of Civil and Building Engineering, Université de Sherbrooke, 2500 boul. de l'Université, Sherbrooke Québec J1K 2R1, Canada.The potential of a laccase from Trametes hirsuta, a hydrolase mixture of amylase, lipase cellulase and protease, was studied for the removal of a set of trace organic contaminants (TrOCs) at 10 µg/L each in a contaminated water. An attempt was also made to develop a combination of cross-linked enzyme aggregates (combi-CLEA) from the two groups of enzymes. After a 24-hour in batch treatments at 22°C and pH 7, compounds such as acetaminophen and levothyroxine showed a decrease in concentration of 97% and 99% respectively, in presence of laccase, while amlodipine, levothyroxine, and trimethoprim were removed at 98%, 99% and 99% respectively, when the contaminated water was treated with the hydrolase mixture. The study of the impact of pH, temperature and contact time revealed that the most favorable temperatures were 22°C and 30°C, but no clear correlation was found between pH and removal in the pH range of 6-8, although high removals were achieved at these pHs. Finally, except few compounds such as acetaminophen, the TrOC removal after 24 h was higher than that obtained in 4 h.http://www.sciencedirect.com/science/article/pii/S2666765722000783BiocatalystsLaccaseHydrolytic enzymesTrace organic contaminantsLC/MS/MS |
| spellingShingle | Komla Alokpa François Lafortune Hubert Cabana Application of laccase and hydrolases for trace organic contaminants removal from contaminated water Environmental Advances Biocatalysts Laccase Hydrolytic enzymes Trace organic contaminants LC/MS/MS |
| title | Application of laccase and hydrolases for trace organic contaminants removal from contaminated water |
| title_full | Application of laccase and hydrolases for trace organic contaminants removal from contaminated water |
| title_fullStr | Application of laccase and hydrolases for trace organic contaminants removal from contaminated water |
| title_full_unstemmed | Application of laccase and hydrolases for trace organic contaminants removal from contaminated water |
| title_short | Application of laccase and hydrolases for trace organic contaminants removal from contaminated water |
| title_sort | application of laccase and hydrolases for trace organic contaminants removal from contaminated water |
| topic | Biocatalysts Laccase Hydrolytic enzymes Trace organic contaminants LC/MS/MS |
| url | http://www.sciencedirect.com/science/article/pii/S2666765722000783 |
| work_keys_str_mv | AT komlaalokpa applicationoflaccaseandhydrolasesfortraceorganiccontaminantsremovalfromcontaminatedwater AT francoislafortune applicationoflaccaseandhydrolasesfortraceorganiccontaminantsremovalfromcontaminatedwater AT hubertcabana applicationoflaccaseandhydrolasesfortraceorganiccontaminantsremovalfromcontaminatedwater |