A Novel Transcription Factor VPA0041 Was Identified to Regulate the Swarming Motility in <em>Vibrio parahaemolyticus</em>
<i>Vibrio parahaemolyticus</i> can change their usual lifestyle of surviving in an aqueous environment attached to a host, wherein both swimming motility and swarming motility play important roles in lifestyle changes, respectively. VPA0041 is a novel transcription factor involved in reg...
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MDPI AG
2022-04-01
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| Series: | Pathogens |
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| Online Access: | https://www.mdpi.com/2076-0817/11/4/453 |
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| author | Mingzhu Li Hongmei Meng Yang Li Dan Gu |
| author_facet | Mingzhu Li Hongmei Meng Yang Li Dan Gu |
| author_sort | Mingzhu Li |
| collection | DOAJ |
| description | <i>Vibrio parahaemolyticus</i> can change their usual lifestyle of surviving in an aqueous environment attached to a host, wherein both swimming motility and swarming motility play important roles in lifestyle changes, respectively. VPA0041 is a novel transcription factor involved in regulating the swarming ability of <i>V. parahaemolyticus</i>. The deletion of the <i>vpa0041</i> gene resulted in the loss of swarming motility in the brain heart infusion (BHI) agars, while the swimming motility was unaffected by VPA0041. Transmission electron microscope (TEM) assays showed that no flagellum was found around the bacterial cells. RNA-sequencing (RNA-Seq) analysis revealed that VPA0041 regulated 315 genes; 207 genes were up-regulated, and 108 genes were down-regulated. RNA-seq results indicated that the lateral flagellar genes were down-regulated by VPA0041, which was confirmed by real-time quantitative reverse transcription-polymerase chain reaction (qRT-PCR). Electrophoretic mobility shift assays (EMSA) demonstrated that VPA0041 directly bound to the promoters of <i>vpa0264</i>, <i>vpa1548,</i> and <i>vpa1550</i> to regulate the expression of the lateral flagellar genes. Our results demonstrated that the transcription factor VPA0041 could directly regulate the expression of lateral flagellar genes to mediate the swarming motility in <i>V. parahaemolyticus</i>. |
| first_indexed | 2024-03-09T04:20:00Z |
| format | Article |
| id | doaj.art-4ee19c507fb44e0f976668f2e60afba8 |
| institution | Directory Open Access Journal |
| issn | 2076-0817 |
| language | English |
| last_indexed | 2024-03-09T04:20:00Z |
| publishDate | 2022-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Pathogens |
| spelling | doaj.art-4ee19c507fb44e0f976668f2e60afba82023-12-03T13:49:02ZengMDPI AGPathogens2076-08172022-04-0111445310.3390/pathogens11040453A Novel Transcription Factor VPA0041 Was Identified to Regulate the Swarming Motility in <em>Vibrio parahaemolyticus</em>Mingzhu Li0Hongmei Meng1Yang Li2Dan Gu3Jiangsu Co-Innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou University, Yangzhou 225009, ChinaJiangsu Co-Innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou University, Yangzhou 225009, ChinaJiangsu Co-Innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou University, Yangzhou 225009, ChinaJiangsu Co-Innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou University, Yangzhou 225009, China<i>Vibrio parahaemolyticus</i> can change their usual lifestyle of surviving in an aqueous environment attached to a host, wherein both swimming motility and swarming motility play important roles in lifestyle changes, respectively. VPA0041 is a novel transcription factor involved in regulating the swarming ability of <i>V. parahaemolyticus</i>. The deletion of the <i>vpa0041</i> gene resulted in the loss of swarming motility in the brain heart infusion (BHI) agars, while the swimming motility was unaffected by VPA0041. Transmission electron microscope (TEM) assays showed that no flagellum was found around the bacterial cells. RNA-sequencing (RNA-Seq) analysis revealed that VPA0041 regulated 315 genes; 207 genes were up-regulated, and 108 genes were down-regulated. RNA-seq results indicated that the lateral flagellar genes were down-regulated by VPA0041, which was confirmed by real-time quantitative reverse transcription-polymerase chain reaction (qRT-PCR). Electrophoretic mobility shift assays (EMSA) demonstrated that VPA0041 directly bound to the promoters of <i>vpa0264</i>, <i>vpa1548,</i> and <i>vpa1550</i> to regulate the expression of the lateral flagellar genes. Our results demonstrated that the transcription factor VPA0041 could directly regulate the expression of lateral flagellar genes to mediate the swarming motility in <i>V. parahaemolyticus</i>.https://www.mdpi.com/2076-0817/11/4/453<i>Vibrio parahaemolyticus</i>VPA0041swarming motilitylateral flagellar systemRNA-seq |
| spellingShingle | Mingzhu Li Hongmei Meng Yang Li Dan Gu A Novel Transcription Factor VPA0041 Was Identified to Regulate the Swarming Motility in <em>Vibrio parahaemolyticus</em> Pathogens <i>Vibrio parahaemolyticus</i> VPA0041 swarming motility lateral flagellar system RNA-seq |
| title | A Novel Transcription Factor VPA0041 Was Identified to Regulate the Swarming Motility in <em>Vibrio parahaemolyticus</em> |
| title_full | A Novel Transcription Factor VPA0041 Was Identified to Regulate the Swarming Motility in <em>Vibrio parahaemolyticus</em> |
| title_fullStr | A Novel Transcription Factor VPA0041 Was Identified to Regulate the Swarming Motility in <em>Vibrio parahaemolyticus</em> |
| title_full_unstemmed | A Novel Transcription Factor VPA0041 Was Identified to Regulate the Swarming Motility in <em>Vibrio parahaemolyticus</em> |
| title_short | A Novel Transcription Factor VPA0041 Was Identified to Regulate the Swarming Motility in <em>Vibrio parahaemolyticus</em> |
| title_sort | novel transcription factor vpa0041 was identified to regulate the swarming motility in em vibrio parahaemolyticus em |
| topic | <i>Vibrio parahaemolyticus</i> VPA0041 swarming motility lateral flagellar system RNA-seq |
| url | https://www.mdpi.com/2076-0817/11/4/453 |
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