<named-content content-type="genus-species">Escherichia coli</named-content> ZipA Organizes FtsZ Polymers into Dynamic Ring-Like Protofilament Structures
ABSTRACT ZipA is an essential cell division protein in Escherichia coli. Together with FtsA, ZipA tethers dynamic polymers of FtsZ to the cytoplasmic membrane, and these polymers are required to guide synthesis of the cell division septum. This dynamic behavior of FtsZ has been reconstituted on plan...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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American Society for Microbiology
2018-07-01
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| Series: | mBio |
| Subjects: | |
| Online Access: | https://journals.asm.org/doi/10.1128/mBio.01008-18 |
| _version_ | 1818825936286515200 |
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| author | Marcin Krupka Marta Sobrinos-Sanguino Mercedes Jiménez Germán Rivas William Margolin |
| author_facet | Marcin Krupka Marta Sobrinos-Sanguino Mercedes Jiménez Germán Rivas William Margolin |
| author_sort | Marcin Krupka |
| collection | DOAJ |
| description | ABSTRACT ZipA is an essential cell division protein in Escherichia coli. Together with FtsA, ZipA tethers dynamic polymers of FtsZ to the cytoplasmic membrane, and these polymers are required to guide synthesis of the cell division septum. This dynamic behavior of FtsZ has been reconstituted on planar lipid surfaces in vitro, visible as GTP-dependent chiral vortices several hundred nanometers in diameter, when anchored by FtsA or when fused to an artificial membrane binding domain. However, these dynamics largely vanish when ZipA is used to tether FtsZ polymers to lipids at high surface densities. This, along with some in vitro studies in solution, has led to the prevailing notion that ZipA reduces FtsZ dynamics by enhancing bundling of FtsZ filaments. Here, we show that this is not the case. When lower, more physiological levels of the soluble, cytoplasmic domain of ZipA (sZipA) were attached to lipids, FtsZ assembled into highly dynamic vortices similar to those assembled with FtsA or other membrane anchors. Notably, at either high or low surface densities, ZipA did not stimulate lateral interactions between FtsZ protofilaments. We also used E. coli mutants that are either deficient or proficient in FtsZ bundling to provide evidence that ZipA does not directly promote bundling of FtsZ filaments in vivo. Together, our results suggest that ZipA does not dampen FtsZ dynamics as previously thought, and instead may act as a passive membrane attachment for FtsZ filaments as they treadmill. IMPORTANCE Bacterial cells use a membrane-attached ring of proteins to mark and guide formation of a division septum at midcell that forms a wall separating the two daughter cells and allows cells to divide. The key protein in this ring is FtsZ, a homolog of tubulin that forms dynamic polymers. Here, we use electron microscopy and confocal fluorescence imaging to show that one of the proteins required to attach FtsZ polymers to the membrane during E. coli cell division, ZipA, can promote dynamic swirls of FtsZ on a lipid surface in vitro. Importantly, these swirls are observed only when ZipA is present at low, physiologically relevant surface densities. Although ZipA has been thought to enhance bundling of FtsZ polymers, we find little evidence for bundling in vitro. In addition, we present several lines of in vivo evidence indicating that ZipA does not act to directly bundle FtsZ polymers. |
| first_indexed | 2024-12-19T00:19:41Z |
| format | Article |
| id | doaj.art-4ee3170ccdf740c9a311cdee9610dde5 |
| institution | Directory Open Access Journal |
| issn | 2150-7511 |
| language | English |
| last_indexed | 2024-12-19T00:19:41Z |
| publishDate | 2018-07-01 |
| publisher | American Society for Microbiology |
| record_format | Article |
| series | mBio |
| spelling | doaj.art-4ee3170ccdf740c9a311cdee9610dde52022-12-21T20:45:37ZengAmerican Society for MicrobiologymBio2150-75112018-07-019310.1128/mBio.01008-18<named-content content-type="genus-species">Escherichia coli</named-content> ZipA Organizes FtsZ Polymers into Dynamic Ring-Like Protofilament StructuresMarcin Krupka0Marta Sobrinos-Sanguino1Mercedes Jiménez2Germán Rivas3William Margolin4Department of Microbiology and Molecular Genetics, McGovern Medical School, Houston, Texas, USACentro de Investigaciones Biológicas, Consejo Superior de Investigaciones Científicas (CSIC), Madrid, SpainCentro de Investigaciones Biológicas, Consejo Superior de Investigaciones Científicas (CSIC), Madrid, SpainCentro de Investigaciones Biológicas, Consejo Superior de Investigaciones Científicas (CSIC), Madrid, SpainDepartment of Microbiology and Molecular Genetics, McGovern Medical School, Houston, Texas, USAABSTRACT ZipA is an essential cell division protein in Escherichia coli. Together with FtsA, ZipA tethers dynamic polymers of FtsZ to the cytoplasmic membrane, and these polymers are required to guide synthesis of the cell division septum. This dynamic behavior of FtsZ has been reconstituted on planar lipid surfaces in vitro, visible as GTP-dependent chiral vortices several hundred nanometers in diameter, when anchored by FtsA or when fused to an artificial membrane binding domain. However, these dynamics largely vanish when ZipA is used to tether FtsZ polymers to lipids at high surface densities. This, along with some in vitro studies in solution, has led to the prevailing notion that ZipA reduces FtsZ dynamics by enhancing bundling of FtsZ filaments. Here, we show that this is not the case. When lower, more physiological levels of the soluble, cytoplasmic domain of ZipA (sZipA) were attached to lipids, FtsZ assembled into highly dynamic vortices similar to those assembled with FtsA or other membrane anchors. Notably, at either high or low surface densities, ZipA did not stimulate lateral interactions between FtsZ protofilaments. We also used E. coli mutants that are either deficient or proficient in FtsZ bundling to provide evidence that ZipA does not directly promote bundling of FtsZ filaments in vivo. Together, our results suggest that ZipA does not dampen FtsZ dynamics as previously thought, and instead may act as a passive membrane attachment for FtsZ filaments as they treadmill. IMPORTANCE Bacterial cells use a membrane-attached ring of proteins to mark and guide formation of a division septum at midcell that forms a wall separating the two daughter cells and allows cells to divide. The key protein in this ring is FtsZ, a homolog of tubulin that forms dynamic polymers. Here, we use electron microscopy and confocal fluorescence imaging to show that one of the proteins required to attach FtsZ polymers to the membrane during E. coli cell division, ZipA, can promote dynamic swirls of FtsZ on a lipid surface in vitro. Importantly, these swirls are observed only when ZipA is present at low, physiologically relevant surface densities. Although ZipA has been thought to enhance bundling of FtsZ polymers, we find little evidence for bundling in vitro. In addition, we present several lines of in vivo evidence indicating that ZipA does not act to directly bundle FtsZ polymers.https://journals.asm.org/doi/10.1128/mBio.01008-18Escherichia colicell divisioncell membranesftsZpolymerszipA |
| spellingShingle | Marcin Krupka Marta Sobrinos-Sanguino Mercedes Jiménez Germán Rivas William Margolin <named-content content-type="genus-species">Escherichia coli</named-content> ZipA Organizes FtsZ Polymers into Dynamic Ring-Like Protofilament Structures mBio Escherichia coli cell division cell membranes ftsZ polymers zipA |
| title | <named-content content-type="genus-species">Escherichia coli</named-content> ZipA Organizes FtsZ Polymers into Dynamic Ring-Like Protofilament Structures |
| title_full | <named-content content-type="genus-species">Escherichia coli</named-content> ZipA Organizes FtsZ Polymers into Dynamic Ring-Like Protofilament Structures |
| title_fullStr | <named-content content-type="genus-species">Escherichia coli</named-content> ZipA Organizes FtsZ Polymers into Dynamic Ring-Like Protofilament Structures |
| title_full_unstemmed | <named-content content-type="genus-species">Escherichia coli</named-content> ZipA Organizes FtsZ Polymers into Dynamic Ring-Like Protofilament Structures |
| title_short | <named-content content-type="genus-species">Escherichia coli</named-content> ZipA Organizes FtsZ Polymers into Dynamic Ring-Like Protofilament Structures |
| title_sort | named content content type genus species escherichia coli named content zipa organizes ftsz polymers into dynamic ring like protofilament structures |
| topic | Escherichia coli cell division cell membranes ftsZ polymers zipA |
| url | https://journals.asm.org/doi/10.1128/mBio.01008-18 |
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