Tissue Dissociation and Primary Cells Isolation Using Recombinant Collagenases Class I and II
Collagenases class I (Col G) and class II (Col H) currently available for tissue dissociation are produced from Clostridium histolyticum (human pathogen) strains. In the processes of extraction of the cells from the tissue, combined activity of both classes of enzymes is required. CI and CII are com...
Main Authors: | M. Salamone, S. Saladino, M. Pampalone, S. Campora, G. Ghersi |
---|---|
Format: | Article |
Language: | English |
Published: |
AIDIC Servizi S.r.l.
2014-09-01
|
Series: | Chemical Engineering Transactions |
Online Access: | https://www.cetjournal.it/index.php/cet/article/view/5647 |
Similar Items
-
Isolation of Adult Rat Cardiomyocytes Using Recombinant Collagenases
by: S. Campora, et al.
Published: (2018-05-01) -
Biochemical Comparison Between Clostridium Hystoliticum Collagenases G and H obtained by DNA Recombinant and Extractive Procedures
by: M. Salamone, et al.
Published: (2012-06-01) -
Adjustment of the ratio between collagenase class II and I improves islet isolation outcome.
by: Brandhorst, D, et al.
Published: (2005) -
Successful human islet isolation utilizing recombinant collagenase.
by: Brandhorst, H, et al.
Published: (2003) -
Technical advantage of recombinant collagenase for isolation of muscle stem cells
by: Kana Ishii, et al.
Published: (2017-12-01)